Case 1:18-cv-01363-CFC Document 79-1 Filed 03/22/19 Page 1 of 13 PageID #:
`9412
`
`Curriculum Vitae
`
`Name:
`
`Hansjörg Hauser
`
`Date of Birth:
`
`January 2, 1949
`
`Department:
`
`Education:
`1974
`1977
`
`Department of Scientific Strategy
`Helmholtz Centre for Infection Research (HZI)
`Inhoffenstraße 7, 38124 Braunschweig, Germany
`Phone: ++49 531 6181-5000
`Fax: ++49 531 6181-805000
`e-mail : hha@helmholtz-hzi.de
`
`Food Science, Universität Stuttgart-Hohenheim, Germany
`Ph.D. in Biology (Dr. rer. nat.); University of Konstanz, Konstanz,
`Germany. Advisor: Rolf Knippers
`
`Postdoctoral Training:
` 1978-80
`Max-Planck-Institut für Molekulare Genetik, Berlin (with Günter Schütz)
`1980
`German Cancer Centre (DKFZ), Heidelberg (with Günter Schütz)
`
` Fellowships:
`1982
`
`EMBO fellowship at the MRC Institute Mill Hill (Dept of R. Flavell)
`
`1986-93
`
`1994-14
`
`Academic Appointments:
`1981-
`Staff Scientist, Gesellschaft f. Biotechnologische Forschung (GBF),
`Braunschweig, Germany
`Head of Research Group “Genetics of Eukaryotes”, GBF,
`Braunschweig
` Head, Dept. of Gene Regulation and Differentiation, GBF
`Braunschweig
` Head, Div. of Molecular Biotechnology, Helmholtz Centre for Infection
`Research (HZI) Braunschweig
`Dept. of Scientific Strategy, HZI, Braunschweig
`2014-
`Since 2004 Guest professorship; University of Lisbon
`1986
` Director and Professor; BFA Tübingen (not accepted)
`1993
`C3 Professorship, University of Bielefeld (not accepted)
`1996
` C4 Professorship, University of Stuttgart-Hohenheim (not accepted)
`
`1995-
`
`1
`
`

`

`Case 1:18-cv-01363-CFC Document 79-1 Filed 03/22/19 Page 2 of 13 PageID #:
`9413
`
`Other professional/academic positions:
`1989-2001
`Scientific advisor for the German-Israeli-Foundation DISNAT.
`1992-1997
`Scientific advisor, HKFG (Hamburger Kommission für
`Gentechnologie).
`1994-2014 Member and Chair “Medical Biotechnology” DECHEMA
`Since 1995 Advisor; Bundesinstitut für Arzneimittelzulassung
`Since 1992 Member, vice president and president of ESACT (European Society of
`Animal Cell Technology)
` Member and Chairman of Scientific Committee, DSMZ (German
`Collection of Microorganisms and Cell Lines)
` Board member, Excellence Cluster REBIRTH, Medical University
`Hannover
`Since 1996 Scientific advisor to diverse companies
` Reviewer for Scientific Journals and Research Foundations from
`Germany, Europe, USA and Israel
`Coordinator and partner in EU projects
`
`Since 2007
`
`Since 2013
`
`Activities at HZI:
`
`
`
`Deputy of the Scientific Director and procuration (until 4/2014);
`Head of Department;
`Member of the HZI Steering Committee
`
`Research interests
`
`Hansjörg Hauser’s expertise is molecular biology with emphasis on gene regulation.
`His activities in mammalian cell research concern the genetic engineering of cells
`with regard to protein expression (cell line development). He is involved in the
`development of technologies for cell screening and its rational design of expression
`parameters. The applications concern protein production and gene/cell therapies. His
`interests in basic research concern regulation of viral infection and the interferon
`system.
`
`2
`
`

`

`Case 1:18-cv-01363-CFC Document 79-1 Filed 03/22/19 Page 3 of 13 PageID #:
`9414
`
`Hanjörg Hauser
`
`ACT-PUBLICATIONS
`
`
`
`Hauser, H., Gross, G., Bruns, W., Hochkeppel, H.K., Mayr, U. and Collins, J.: Inducibility of the
`human interferon ß gene in mouse L cell clones. Nature 297, 650-654 (1982)
`
`
`Hauser, H., Dinter, H. and Collins, J.: Construction of interferon-producing mouse cell lines by genetic
`engineering. 3rd. Eur. Congress on Biotechnology, Verlag Weinheim, Vol. III, 279-283 (1984)
`
`Delzer, J., Hauser, H. and Lehmann, J.: Production of human interferon-ß in mouse L cells. 3rd. Eur.
`Congress on Biotech-nology, Verlag Weinheim, Vol. I, 179-181 (1984)
`
`Conradt, H.S., Ausmeier, M., Dittmar, K.E.J., Hauser, H. and Lindenmaier, W.: Secretion of
`glycosylated human Il-2 by recombinant mammalian cell lines. Carbohydrate Res. 149, 443-450
`(1986)
`
`Reiser, W. and Hauser, H.: Recombinant human Interferon-beta from mammalian cell lines. Drug Res.
`37, 482-485 (1987)
`
`
`Wirth, M., Reiser, W., Zettlmeißl, G., Conradt, H.S., Cao, J.H., Morelle, C. and Hauser, H.:
`Recombinant animal cell lines for production of glycoproteins, in: Modern Approaches to Animal Cell
`Technology, Hrsg. R.E.Spier, J.B.Griffiths, Butterworths, 182-198 (1987)
`
`Beckers, T., Hauser, H., Hüsken, D. and Engels, J.W.: Expression and characterization of a des- Met
`mutant Interleukin-2 receptor (Tac-protein) with Interleukin-2 binding affinity. J. Biol. Chem. 236, 8359-
`8365 (1988)
`
`
`Conradt, H.S., Hauser, H., Lorenz, C. Mohr, H. and Plessing, A.: Posttranslational modification of
`Interleukin-2 is a late event during activation of human T lymphocytes by ionophore A23187 and
`phorbol ester. Biochem. Biophys. Res. Commun., 150, 97-103 (1988)
`
`
`Zettlmeißl, G. Wirth, M., Hauser, H. and Küpper, H.: Efficient expression system for human
`antithrombin III in baby hamster kidney cells. Behring-Institut-Mitteilungen 82, 26-34 (1988)
`
`Wirth, M., Bode, J., Zettlmeißl, G. and Hauser, H.: Isolation of overproducing recombinant
`mammalian cell lines by a rapid and simple selection procedure. Gene, 73, 419-426 (1988)
`
`
`

`

`Case 1:18-cv-01363-CFC Document 79-1 Filed 03/22/19 Page 4 of 13 PageID #:
`9415
`
`Hanjörg Hauser
`
`
`Wirth, M., Li, S.-Y., Schumacher, L., Lehmann, J., Zettlmeißl, G. and Hauser, H.: Screening for and
`fermentation of high producer cell clones from recombinant BHK cells, in: Advances in Animal Cell
`Biology and Technology for Bioprocesses, eds. Spier, R.E., Griffiths, J.B., Stephenne, J. and Crooy,
`P.J., Butterworths, 44-50, (1989)
`
`Dittmar, K.E.J., Conradt, H.S., Hauser, H., Hofer, B. and Lindenmaier, W.: Human glycoproteins and
`derived variants from recombinant mammalian cell lines, in: Protein design, eds. Blöcker, H., Collins,
`J., Schmid, R. and Schomburg, D., VCH, Weinheim, 12, 145-156 (1989)
`
`Conradt, H.S., Nimtz, M., Hoppe, J. and Hauser, H.: Expression of human IL-2 in recombinant BHK,
`Ltk- and CHO cells: Structure of O-linked carbohydrates and their localisation within the polypeptide,
`J. Biol. Chem. 264, 17368-17373 (1989)
`
`Eichner, W., Jäger, V., Herbst, D., Hauser, H. and Hoppe, J.: Large-scale preparation of recombinant
`platelet-derived growth factor AA secreted from recombinant baby hamster kidney cells. Eur. J.
`Biochem. 185, 135-140 (1989)
`
`Conradt, H.S., Hofer, B. and Hauser, H.: Expression of human glycoproteins in recombinant
`mammalian cells: Towards genetic engineering of N- and O-glycoproteins. TIGG 2, 168-181 (1990)
`
`Beckers, T., Hauser, H., Hüsken, D. and Engels, J.W.: Analysis of soluble des-methionine mutant
`Interleukin-2 Receptor -chain (Tac-protein) produced by transfected mammalian cells. Eur. J.
`Biochem. 189, 657-665 (1990)
`
`Wirth, M., Höxter, M., Morelle, C. and Hauser, H.: Bicistronic expression vectors facilitate screening
`for overexpressing mammalian cells, in DECHEMA Biotechnology Conferences, eds. Behrens, D. and
`Krämer, P., VCH, Weinheim 4, 69-73 (1990)
`
`Wirth, M. Schumacher, L. and Hauser, H.: Use of dicistronic transcription units for the correlated
`expression of two genes in mammalian cells, in: Production of biologicals from animal cells in
`
`culture. eds. Spier, R. E., Griffiths, J.B.and Meignier, B., Butterworth-Heinemann, 338-343 (1991)
`
`Wirth, M., Heinemeyer, T. Berthold, E. and Hauser, H.: Rapid and sensitive detection of
`mycoplasmas and retroviruses in animal cell culture. eds. Krupa, A. and Driesel, A.J. Dechema
`Biotechnology Conferences, VCH, Weinheim 5, 247-250 (1992)
`
`Wirth, M. and Hauser, H.: Genetic engineering of animal cells, in: Biotechnology Vol 2 (ed.
`Pühler, A.) VCH, Weinheim 663-744 (1993)
`
`

`

`Case 1:18-cv-01363-CFC Document 79-1 Filed 03/22/19 Page 5 of 13 PageID #:
`9416
`
`Hanjörg Hauser
`
`
`Dirks, W., Wirth, M. and Hauser, H.: Bicistronic transcription units for gene expression in mammalian
`cells. Gene 128 247-249 (1993)
`
`Wirth, M. and Hauser, H.: Genetic engineering of animal cells, in: Genetic engineering of animals. (ed.
`Pühler, A.) VCH, Weinheim 1-82 (1993)
`
`Hopert, A., Uphoff, C. C., Gognac, S. M., Wirth, M., Hauser, H. and Drexler, H. G.: Specificity and
`sensitivity of polymerase chain reaction (PCR) in comparison with other methods for the detection of
`mycoplasma contamination in cell lines. J. Immunol. Methods, 164 91-100 (1993)
`
`
`Hopert, A., Uphoff, C.C., Wirth, M., Hauser, H. and Drexler, H.G.: Mycoplasma detection by PCR
`analysis. In Vitro Cell. Dev. Biol. 29A 819-821 (1993)
`
`Wirth, M., Grannemann, R. and Hauser, H.: Screening retroviral helper cells for highly efficient virus
`production using a combined selection procedure. J. Virology, 68 566-569
`(1994)
`
`Racher, A. J., Moreira, J. L., Alves, P. M., Wirth, M., Weidle, U. H., Hauser, H., Carrondo, M. J. T. and
`Griffiths, J. B.: Expression of recombinant antibody and secreted alkaline phosphatase im mammalian
`cells. Influence of cell line and culture system upon production kinetics. Applied Microbiology and
`Biotechnology, 40 851-856 (1994)
`
`Kollmus, H., Honigman, A., Panet, A. and Hauser, H.: The sequences and distance between two cis-
`acting signals determine the efficiency of ribosomal frameshifting in Human Immunodeficiency Virus
`type 1 and Human T-Cell Leukemia Virus type II in vivo J. Virol., 68 (1994)
`
`Dirks, W. and Hauser, H.: Equimolar expression of two protein chains in mammalian cells. In: Animal
`Cell Technology: Products of Today, Prospects for Tomorrow. (Spier, R., Griffiths, B., and
`
`Berthold, W., eds), Butterworths, Oxford, 610-616 (1994)
`
`Dirks, W., Mielke, C., Karreman, S., Haase, B., Wirth, M., Lindenmaier, W. and Hauser,H.: Applications
`of expression vectors containing bicistronic transcription units in mammalian cells. in: Cell culture in
`pharmaceutical research. (Fusenig, N.E.,und Graf, H., eds) Springer, Heidelberg, 239-266 (1994)
`
`Dirks, W., Schaper, F., Kirchhoff, S., Morelle, C. and Hauser, H.: A multifunctional vector family for
`gene expression in mammalian cells. Gene, 149, 387-388 (1994)
`
`
`

`

`Case 1:18-cv-01363-CFC Document 79-1 Filed 03/22/19 Page 6 of 13 PageID #:
`9417
`
`Hanjörg Hauser
`
`
`Wirth, M., Berthold, E., Grashoff, M., Pfützner, H., Schubert, U. and Hauser, H.: Detection of
`mycoplasma contaminations by the polymerase chain reaction. Cytotechnology, 16, 67-77 (1994)
`
`
`Wirth, M., Grashoff, M., Schumacher, L. and Hauser, H.: Mycoplasma detection by the Mycoplasma
`PCR ELISA. Biochemica, 3, 33-35 (1995)
`
`Köster, M., Kirchhoff, S., Schaper, F. and Hauser, H.: Proliferation control of mammalian cells by the
`tumor suppressor IRF-1. Cytotechnology, 18, 67-75 (1995)
`
`
`Klehr-Wirth, D., Wirth, M., Grannemann, R., Münk, C. and Hauser, H.: Accidental formation of
`replication-competent viruses from gene transfer by retroviral vectors, in Biosafety of transgenic
`organism, horizontal gene transfer and expression of transgenes. (Schmidt, E. R., Hankelen, T., eds)
`Springer-Verlag, Heidelberg, 105-115 (1995)
`
`Kirchhoff, S., Köster, M., Wirth, M., Schaper, F., Gossen, M., Bujard, H. and Hauser, H.:
`Identification of mammalian cell clones exhibiting highly regulated expression from inducible
`promoters. TIG, 11, 219-220 (1995)
`
`Gross, G. and Hauser, H.: Gene analysis by heterologous expression. J. Biotechnology,
`Biotechnology, 41, 91-110 (1995)
`
`
`Karreman, S., Hauser, H. and Karreman, C.: On the use of double FRT sites in the LTR of
`retroviruses for the construction of high producer cell lines. Nucleic Acids Res. 24, 1616-1624
`(1996)
`
`Wirth, M., Grashoff, M., Schumacher, L. and Hauser, H.
`Mycoplasma detection by the Mycoplasma PCR ELISA. Biochemica 98, 30_32 (1996).
`
`
`Klehr-Wirth, D., Wirth, M., Grannemann, R., Münk, C. and Hauser, H.: Accidental formation of
`replication-competent viruses from gene transfer by retroviral vectors, in Biosafety of transgenic
`organisms, horizontal gene transfer and expression of transgenes. (Schmidt, E.R., Henkelen, T., eds.)
`Springer, Heidelberg, 105-115 (1996)
`
`
`Karreman, C., Karreman, S. and Hauser, H. In Construction of recombinant cell lines with defined
`properties using FLP recombinase riven gene replacement, in Animal Cell Technology: From Vaccines
`to Genetic Medicine (Carrondo, M.J.T., Griffiths, B., Moreira, J.L.P., eds.) Kluwer Academic
`Publishers, 511-518 (1997)
`
`

`

`Case 1:18-cv-01363-CFC Document 79-1 Filed 03/22/19 Page 7 of 13 PageID #:
`9418
`
`Hanjörg Hauser
`
`
`Wirth, M., Heinemeyer, T., Schumacher, L., Klingenhoff, A., Hansen, W., Suemeghy, Z., Huber, G.,
`Schläpfer, B.S., Wirth, D. and Hauser, H.: PCR detection of murine retroviruses and characterization of
`a new xenotropic retrovirus from myeloma cells, in Animal Cell Technology: From Vaccines to Genetic
`Medicine (Carrondo, M.J.T., Griffiths, B., Moreira, J.L.P., eds.) Kluwer Academic Publishers, 37-42
`(1997)
`
`Hauser, H.: Heterologous expression of genes in mammalian cells. In : Mammalian Cell Biotechnology in
`Protein Production. (H. Hauser and R. Wagner, eds.) De Gruyter, Berlin, 1-32 (1997)
`
`Wild, J., Grüner, B., Metzger, K., Kuhröber, A., Pudollek, H.-P., Hauser, H., Schirmbeck, R. and
`Reimann, J.: Polyvalent vaccination against hepatitis B surface and core antigen using a dicistronic
`expression plasmid. Vaccine, 16, 353-360 (1998)
`
`
`Carvalhal, A.V., Moreira, J.L., Müller, P.P., Hauser, H. and Carrondo, M.J.T.: Cell growth inhibition
`by the IRF-1 system, in New developments and new applications in animal cell technology (Merten,
`O.-W., Perrin, P, and Griffiths, B., eds.) Kluwer Academic Publishers 215- 217 (1998)
`
`Müller, P.P., Kirchhoff, S. and Hauser, H.: Sustained expression in proliferation controlled BHK- 21
`cells, in New developments and new applications in animal cell technology (Merten, O.-W., Perrin, P,
`and Griffiths, B., eds.) Kluwer Academic Publishers 209-214 (1998)
`
`Verhoeyen, E., Hauser, H., and Wirth, D.: Efficient targeting of retrovirally FRT tagged chromosomal
`loci, Trends in Genetics, TO1515 (1998)
`
`Fussenegger, M., Bailey, J., Hauser, H. and Mueller, P.P.: Genetic Optimization of Recombinant
`Protein Production by Mammalian Cells. TIBTECH, 17, 35-42 (199Müller, P.P., Carvalhal, A.V.,
`Moreira, J.L., Geserick, C., Schroeder, K., Carrondo, M.J.T. and Hauser. H.: Development of an IRF-
`1-based proliferation control system. system. In Cell Engineering 1 (eds. Al’Rubeai, Betenbaugh,
`Hauser, Jenkins, MacDonald, Merten) 220-238 (1999)
`
`Cayli, A., Hirschmann, F., Wirth, M., Hauser, H. and Wagner, R.: Cell lines with reduced UDP-N-
`Acetyhexosamine pool in the presence of ammonium. Biotech. Bioeng., 65, 192-200 (1999)
`
`Burger, C., Carrondo, M.J.T., Cruz, H., Cuffe, U., Dias, E., Griffiths, J.B., Hayes, K., Hauser, H., Looby,
`D., Mielke, C., Moreira, J.L., Rieke, E., Savage, A., Stacey, G.N., and Welge, T.: An integrated
`
`

`

`Case 1:18-cv-01363-CFC Document 79-1 Filed 03/22/19 Page 8 of 13 PageID #:
`9419
`
`Hanjörg Hauser
`
`
`strategy for the process development of a recombinant antibody-cytokine fusion protein expressed in
`BHK cells. Appl. Microbiol. Biotechnol., 52, 345-353 (1999)
`
`Müller, P.P., Grabenhorst, E., Conradt, H.S. and Hauser, H. : Recombinant glycoprotein product
`quality in proliferation controlled BHK-21 cells. Biotech. Bioeng., 65, 529-536 (1999)
`
`Geserick, C., Schroeder, K., Bonarius, H., Kongerslev, L., Schlenke, P., Hauser, H., and Mueller, P.P.:
`Recombinant pharmaceutical protein overexpression in an IRF-1 proliferation controlled production
`system, In Animal Cell Technology: Products from Cells, Cells as Products (eds.
`Bernard, A., Griffiths, B., Noé, W., Wurm, F.) Kluwer Academic Publishers, The Netherlands, 3-10
`(1999)
`
`Carvalhal, A.V., Moreira, J.L., Cruz, H., Mueller, P.P., Hauser, H. and Carrondo, M.J.T.: Manipulation
`of culture conditions for BHK cell growth inhibition by IRF-1 activation.
`Cytotechnology 32, 135-145 (2000)
`Geserick, C., Bonarius, H.P.J., Kongerslev, L., Hauser, H. and Müller, P.P.: Enhanced productivity
`during controlled proliferation of BHK cells in continuously perfused bioreactors. Biotech. Bioeng.,
`69, 266-274 (2000)
`
`Mielke, C., Tümmler, M, Schübeler, D., von Hoegen, I. and Hauser, H,: Stabilized, long-term
`expression of heteromeric proteins from tricistronic mRNA. Gene, 254, 1-8 (2000)
`
`Kwissa, M., Unsinger, J., Schirmbeck, R., Hauser, H. and Reimann, J.: Polyvalent DNA vaccines with
`bidirectional promoters. J. Mol. Med., 78, 495-506 (2000)
`
`Müller, P., Oumard, A., Wirth, D., Kröger, A., and Hauser, H.: Polyvalent vectors for coexpression of
`multiple genes, in Plasmids for Therapy and Vaccination (ed. Schleef, M.) Wiley-VCH,
`
`Weinheim, 119-136 (2001)
`
`Hennecke, M., van Kampen, J., Metzger, K., Schirmbeck, R., Reimann, J., and Hauser, H.: The strength
`of IRES-driven translation of bicistronic expression vectors depends on the composition of the mRNA.
`Nucleic Acids Res. 29, 3327-3334 (2001)
`
`Verhoeyen, E., Hauser, H., and Wirth, D.: Evaluation of retroviral vector design in defined chromosomal
`loci by Flp-mediated cassette replacement. Human Gene Ther., 12, 933-944 (2001)
`
`
`
`Hauser, H. and Zylstra, G.J.: Mammals, plants, bacteria, and soil: common problems ans solutions in
`gene expression ans analysis. Current Opinion in Biotechnology, 12, 437-438 (2001)
`
`
`

`

`Case 1:18-cv-01363-CFC Document 79-1 Filed 03/22/19 Page 9 of 13 PageID #:
`9420
`
`Hanjörg Hauser
`
`
`Unsinger, J., Kröger, A., Hauser, H., and Wirth, D.: Retroviral vectors for transduction of an
`autoregulated bidirectional expression cassette. Molecular Therapy, 4, 484-489 (2001)
`
`
`Schroeder, K., Koschmieder, S., Ottmann, O.G., Hoelzer, D., Hauser, H., and Mueller, P.P.:
`Coordination of cell growth in cocultures by a genetic proliferation control system. Biotech.
`Bioeng., 78, 346-352 (2002)
`
`
`Mueller, P.P., Wirth, D., Unsinger, U. and Hauser, H.: Genetic approaches to recombinant protein
`production in mammalian cells in Handbook of Industrial Cell Culture: Mammalian, Microbial and Plant
`Cells (ed. V. A. Vinci, S.R. Parekh) Humana Press, 21-50 (2003)
`
`Wirth, D. and Hauser, H.: Flp-mediated integration of expression cassettes into FRT-tagged
`chromosomal loci in mammalian cells. Methods in Molecular Biology, 267, 467-476 (2004)
`
`May, T., Hauser, H., and Wirth, D.:.Transcriptional control of SV40 T-antigen expression allows a
`complete reversion of immortalization. Nucleic Acids Res. 32, 5529-5538 (2004)
`
`
`May, T., Lindenmaier, W., Wirth, D. and Müller, P.P.: Application of a reversible immortalisation
`system for the generation of proliferation-controlled cell lines, Cytotechnology 46, 69-78 (2004)
`
`May, T., Mueller, P.P., Weich, H., Froese, N., Deutsch, U., Wirth, D., Kröger, A. and Hauser, H.:
`Establishment of murine cell lines by constitutive and conditional immortalization. J. Biotech. 120, 99-
`110 (2005)
`
`Coroadinha, A.S., Schucht, R., Norton, L., Wirth, D., Hauser, H., and Carrondo, M.J.T.: The use of
`recombinase cassette exchange in retroviral vector producer cell lines: predictability and
`
`efficiency in transgene exchange. J. Biotech. 124, 457-468 (2006)
`
`
`Schucht, R., Coroadinha, A.S., Verhoeyen, E., Zanta-Boussif, M.A., Carrondo, M.J.T., Hauser, H..,
`and Wirth, D.: A new generation of retroviral producer cells: Predictable and stable virus production
`by Flp mediated site-specific integration of retroviral vectors. Mol.Ther. 14, 285-292 (2006)
`
`May, T., Hauser, H., and Wirth, D.: In vitro expansion of tissue cells by conditional proliferation.
`Methods Molec. Medicine 140, 1-15 (2007)
`
`
`Hauser. H., and Fussenegger, M (eds).: Tissue Engineering. Methods Molec. Medicine 140, (2007)
`
`

`

`Case 1:18-cv-01363-CFC Document 79-1 Filed 03/22/19 Page 10 of 13 PageID #:
`9421
`
`Hanjörg Hauser
`
`
`Fussenegger M, Hauser H. Protein expression by engineering of yeast, plant and animal cells. Curr
`Opin Biotechnol. 18(5), 385-386 (2007)
`
`Wirth, D., Gama-Norton, L., Riemer, P., Sandhu, U., Schucht, R., and Hauser, H.: Road to precision:
`recombinase based targeting technologies for genome engineering. Current Opinion in Biotechnology,
`18, 411-419 (2007)
`
`Cruz, P.E., Coroadinha, A.S., Rodrigues, T., and Hauser, H.: Production of retroviral vectors: from
`the producer cell to the final product. in Gene and Cell Therapy: Therapeutic mechanisms and
`strategies; in “Gene an Cell Therapy – Therapeutic Mechanisms and Strategies” (N.S. Templeton,
`ed.) CRC Press, Boca Raton pp. 17-38 (2008)
`
`Gama-Norton, L.; Riemer, P.; Sandhu, U.; Nehlsen, K.; Schucht, R.; Hauser, H. and Wirth D.:
`Defeating Randomness – Targeted Integration as a Boost for Biotechnology. Cell line development
`and engineering in Cell Engineering, 6, 53-82 (2009)
`
`Nehlsen, K., Schucht, R., da Gama-Norton, L., Krömer, W., Baer, A., Cayli, A., Hauser, H., and Wirth,
`D.: Recombinant protein expression by targeting pre-selected chromosomal loci. BMC Biotech, 9:100
`(2009)
`
`Loew, R., Meyer, Y., Kuehlcke, K., Gamma-Norton, L., Wirth. D., Hauser, H., Stein, S., Grez, M.,
`Thornhill, S., Thrasher, A., Baum, C., and Schambach, A.: A new PG13-based packaging cell line for
`stable production of clinical grade self inactivating γ-retroviral vectors using targeted
`
`integration. Gene Therapy, 17: 272-280 (2010)
`
`
`Nehlsen, K., Herrmann, S., Zauers, J., Hauser, H., and Wirth, D.: Toxin-antitoxin based transgene
`expression in mammalian cells. Nucl. Acids Res., 38:e32 (2010)
`
`
`May, T., Bantner, S., Markusic, D., Seppen, J., Weich, H., Hauser, H., and Wirth, D.: Synthetic gene
`regulation circuits for control of cell expansion, Tissue Eng Part A. (2010) 16, 441-452
`
`Gamma-Norton, L., Hernabb, S., Schucht, R., Coroadinha, A.S., Löw, R., Alves, P.M., Bartholomae,
`C.C., Schmidt, M., Baum, C., Schambach, A., Hauser, H., and Wirth, D.: Retroviral vector performance
`upon integration in to defined chromosomal loci of modular packaging cell lines. Human Gene Therapy,
`21, 979-91 (2010)
`
`Coroadinha, A.S., Cruz, P., Hauser, H., Amaral, A., Alves, P.: Production of Retroviral Vectors:
`Review. Curr.Gene Ther.,10, 456-473 (2010)
`
`

`

`Case 1:18-cv-01363-CFC Document 79-1 Filed 03/22/19 Page 11 of 13 PageID #:
`9422
`
`Hanjörg Hauser
`
`
`Cohen-Haguenauer, O., Creff, N., Cruz, P., Tunc, C., Aïuti, A., Baum, C., Bosch, F., Blomberg, P.,
`Cichutek, K., Collins, M., Danos, O., Dehaut, F., Federspiel, M., Galun, E., Garritsen, H., Hauser, H.,
`Hildebrandt M., Klatzmann, D., Merten, O., Montini, E., O’Brien, T., Panet, A., Rasooly, L., Scherman,
`L., Schmidt, M., Schweitzer, M., Tiberghien, P., Vandendriessche, T., Ziehr, H., Ylä-Herttuala, S., von
`Kalle, C., Gahrton G., and Carrondo, M.: Relevance of an Academic GMP Pan-European Vector Infra-
`structure (PEVI) Curr.Gene Ther., 10, 414-422 (2010)
`
`Carrondo, M.J.C., Panet, A., Wirth, D., Coroadinha, A.S., Cruz, P., Falk, H., Schucht, R., Dupont, F.,
`Geny-Fiamma, C., Merten, O.-W., and Hauser, H.: Integrated strategy for the production of therapeutic
`retroviral vectors. Human Gene Therapy, 22, 370-379 (2011)
`
`Nehlsen, K., Zauers, J., Herrmann, s., Hauser, H., and Wirth, D.: Toxin-antitoxin based stabilization of
`transgene expression in mammalian cells. In Animal Cell Technology: Products from Cells, Cells as
`Products (ed.Jenkins, N.) Springer Press, (2011), in press
`
`Schucht, R., Lydford, S., Andzinski, L., Zauers, J., Cooper, J., Hauser, H., Wirth, D., and May, T.: Rapid
`establishment of GPCR expressing cell lines by site specific integration. J. Biomol.
`Screening 16, 323-332 (2011)
`
`Nehlsen K, da Gama-Norton L, Schucht R, Hauser H, and Wirth D.: Towards rational engineering of
`cells: Recombinant gene expression in defined chromosomal loci. BMC Proc. 5, Suppl 8 (2011)
`Sandhu U, Cebula M, Behme S, Riemer P, Wodarczyk C, Metzger D, Reimann J, Schirmbeck R,
`Hauser H, and Wirth D.: Strict control of transgene expression in a mouse model for sensitive biological
`applications based on RMCE compatible ES cells. Nucleic Acids Res. 39, :e1. (2011)
`
`Coroadinha, A.S., Wirth, D.. Rodrigues, A.F. Gama-Norton, L., Duros, C., Cohen-Haguenauer, O.,
`Alves, P.M., Cruz, P. E., Carrondo, M., and Hauser, H. Modular retroviral producer cell lines in The
`CliniBook: Clinical gene transfer; Odile Cohen-Haguenauer – EDK, Paris, 118-126 (2012)
`
`Gama-Norton, L., Hauser, H., Wirth, D., Restrictions and requirements for stable lentiviral vector
`production in HEK293 cells in The CliniBook: Clinical gene transfer; Odile Cohen-Haguenauer – EDK,
`Paris, 156-159
`
`Schmidt M., Laufs S., Aiuti A., Aubourg, P., Baum, C., Biasco L., Cartier N., Hauser H., Montini E.,
`Moullier P., Snyder R.O., Wirth D., von Kalle C.: Biosafety analysis in preclinical and clinical studies.
`The CliniBook: Clinical gene transfer; Odile Cohen-Haguenauer – EDK Paris, 432-440.
`
`
`

`

`Case 1:18-cv-01363-CFC Document 79-1 Filed 03/22/19 Page 12 of 13 PageID #:
`9423
`
`Hanjörg Hauser
`
`
`Lipps, C., May, T., Hauser, H., and Wirth, D.: Eternity and functionality – rational access to
`physiologically relevant cell lines. Biological Chemistry, 394, 1637-48. doi: 10.1515/hsz-2013- 0158.
`(2013)
`
`Mullen, L., Adams, G., Foster, J., Vessillier, S., Köster, M., Hauser, H., Layward, L., Gould, D.,
`Chernajovsky, Y.: A comparative study of matrix metalloproteinase and aggrecanase mediated
`release of latent cytokines at arthritic joints Ann Rheum Dis doi: 10.1136/annrheumdis-2013-203513
`(2014)
`
`Rand U, Riedel J, Hillebrand U, Shin D, Willenberg S, Behme S, Klawonn F, Köster M, Hauser H, Wirth
`D.: Single-cell analysis reveals heterogeneity in onset of transgene expression from synthetic
`tetracycline-dependent promoters. Biotechnol J., 10:323-31. doi: 10.1002/biot.201400076 (2015)
`
`Spencer S, Gugliotta A, Koenitzer J, Hauser H, Wirth D.: Stability of single copy transgene
`expression in CHOK1 cells is affected by histone modifications but not by DNA methylation. J
`Biotechnol. 195:15-29. doi: 10.1016/j.jbiotec.2014.12.009 (2015)
`
`Hauser, H.: Cell Line Development. in “Animal Cell Culture”, Al-Rubeai (ed) Cell Engineering 9;1-
`23; Springer; DOI 10.1007/978-3-319-10320-4_1 (2015)
`
`Hauser, H. and Wagner, R. (eds) Animal Cell Biotechnology in Biologics Production. W de Gruyter;
`(2015)
`
`Hauser, H.: 3D Cultures Conquer In Vitro Testing. J. Biotechnol., 205, 1-2 DOI:
`10.1016/j.jbiotec.2015.04.008 (2015)
`
`Rand, U., Riedel, J., Hillebrand, U., Shin, D., Willenberg, S., Behme, S., Klawonn, F., Köster, M., Hauser,
`H., and Wirth, D.: Single-cell analysis reveals heterogeneity in onset of transgene expression from
`synthetic tetracycline-dependent promoters. Biotechnol. J., 10, 323–331 DOI 10.1002/biot.201400076
`(2015)
`
`Spencer, S., Gugliotta, A., Goedecke, N., Hauser, H., and Wirth, D.: Epigenetic modulations rendering
`cell-to cell variability and phenotypic metastability. J. Genetics and Genomics, 43, 503- 511 (2016)
`
`Lipps, C., Badar, M., Butueva, M., Dubic, T., Singh, V.V., Rau, S., Weber, A., Kracht, M., Köster, M.,
`May, T., Schulz, T., Hauser, H., and Wirth, D.: Proliferation status defines functional properties of
`endothelial cells. Cellular and Molecular Life Sciences (CMLS), 74, 1319-1333. doi: 10.1007/s00018-
`016-2417-5. (2017)
`
`
`

`

`Case 1:18-cv-01363-CFC Document 79-1 Filed 03/22/19 Page 13 of 13 PageID #:
`9424
`
`Hanjörg Hauser
`
`
`Gödecke, N., Zha, L., Spencer, S., Behme, S., Riemer, P., Rehli, M., Hauser, H., and Wirth, D.:
`Controlled re-activation of epigenetically silenced Tet-promoter driven transgene expression by
`demethylation. Nucl. Acids Res., 45, e147; doi: 10.1093/nar/gkx601 (2017)
`
`Lipps, C., Klein, F., Wahlicht, T., Seiffert, V., Butueva. M., Zauers, J., Herfurth, T., Luckner, M., Köster,
`M., MacLeod, R., Petzold, J., Hühn, J., Yuan, Q., Müller, PP., Dittrich-Breiholz, O., Pufe, T.,
`Beckmann, R., Drescher, W., Riancho, J., Sañudo, C., Korff, T., Opalka, B., Rebmann, V., Göthert,
`J.R., Alves, P.M., Ott, M., Schucht, R., Hauser, H., Wirth, D., May, T.: Expansion of functional,
`personalized cells with specific transgene combinations. Nature Communications, 9, 994 (2018)
`
`
`
`