Case 1:18-cv-01363-CFC Document 1-2 Filed 09/04/18 Page 1 of 67 PageID #: 124
`Case 1:18-cv-01363-CFC Document1-2
`Filed 09/04/18
`Page 1 of 67 PagelD #: 124
`
`EXHIBIT B
`EXHIBIT B
`
`

`

`Case 1:18-cv-01363-CFC Document 1-2 Filed 09/04/18 Page 2 of 67 PageID #: 125
`
`Illll Mllll Ill Illll Illll Illll Ill Illll IM Illll Illll Illlll Illl Illl Illl
`
`US007923221B1
`
`(12) nited States Patent
`Cabilly et al.
`
`(10) Patent No.:
`(45) Date of Patent:
`
`S 7,923,221 B1
`*Apr. 12, 2011
`
`(54) METHODS OF MAKING ANTIBODY HEAVY
`AND LIGHT CHAINS HAVING SPECIFICITY
`FOR A DESIRED ANTIGEN
`
`(75) Inventors:
`
`Shmuel Cabilly, Monrovia, CA (US);
`Herbert L. Heyneker, Burlingame, CA
`(US); William E. Holmes, Pacifica, CA
`(US); Arthur D. Riggs, La Verne, CA
`(US); Ronald B. Wetzel, San Francisco,
`CA (US)
`
`(73)
`
`Assignees: Genentech, Inc, South San Francisco,
`CA (US); City of Hope, Duarte, CA
`(US)
`
`(*) Notice:
`
`Subject to any disclaimer, the term of this
`patent is extended or adjusted under 35
`U.S.C. 154(b) by 0 days.
`
`This patent is subject to a terminal dis-
`claimer.
`
`(21) Appl. No.: 08/422,187
`
`(22) Filed:
`
`Apr. 13, 1995
`
`Related U.S. Application Data
`
`(63)
`
`Continuation of application No. 07/205,419, filed on
`Jun. 10, 1988, now Pat. No. 6,331,415, which is a
`continuation of application No. 06/483,457, filed on
`Apr. 8, 1983, now Pat. No. 4,816,567.
`
`(51) Int. Cl.
`C12N 15/13
`(2006.01)
`C12N 15/00
`(2006.01)
`C12N 15/63
`(2006.01)
`(52) U.S. CI .................. 435/69.6; 435/252.1; 435/252.3;
`435/252.33; 435/254.11; 435/254.21; 435/69.1;
`435/69.7; 435/70.1; 435/70.21; 435/71.1;
`435/320.1;435/455; 435/483; 435/485; 435/440;
`435/433; 435/438
`(58) Field of Classification Search ................. 435/69.6,
`435/69.1,325, 252.3; 530/387.3
`See application file for complete search history.
`
`(56)
`
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`Primary Examiner Phillip Gambel
`(74) Attorney, Agent, or Firm Sidley Austin LLP
`
`(57)
`
`ABSTRACT
`
`The invention relates to processes for producing an immuno-
`globulin or an immunologically functional immunoglobulin
`fragment containing at least the variable domains of the
`immunoglobulin heavy and light chains. The processes can
`use one or more vectors which produce both the heavy and
`light chains or fragments thereof in a single cell. The inven-
`tion also relates to the vectors used to produce the immuno-
`globulin or fragment, and to cells transformed with the vec-
`tors.
`
`47 Claims, 19 Drawing Sheets
`
`

`

`Case 1:18-cv-01363-CFC Document 1-2 Filed 09/04/18 Page 3 of 67 PageID #: 126
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`US 7,923,221 B1
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`FOREIGN PATENT DOCUMENTS
`
`AU
`AU
`AU
`AU
`EP
`EP
`EP
`EP
`EP
`EP
`EP
`EP
`EP
`EP
`EP
`EP
`EP
`EP
`EP
`EP
`EP
`EP
`EP
`EP
`EP
`EP
`EP
`EP
`EP
`EP
`EP
`EP
`EP
`EP
`EP
`EP
`EP
`EP
`EP
`EP
`EP
`EP
`EP
`GB
`GB
`GB
`GB
`GB
`JP
`WO
`WO
`
`12417/83
`B-26429/84
`46556/85
`65981/86
`37723
`037723
`041313
`41313
`41767
`041767
`044722
`55945
`57107
`057107
`060057
`068763
`68763
`073656
`73656
`75444
`075444
`A-073656
`88994
`093619
`102634
`0 120 694
`120694
`0 125 023 B1
`125023 B1
`0171 496
`173494
`177343
`194276
`196864
`234592
`255694
`36776
`324162
`114506
`0 365 997
`088994
`550400
`481790 B1
`2068969
`8308235
`8422238
`9022543
`08235
`62 201 581
`WO 81/02426
`WO 82/03088
`
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`10/1984
`3/1986
`5/1987
`10/1981
`10/1981
`12/1981
`12/1981
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`8/1982
`8/1982
`9/1982
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`1/1983
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`3/1984
`10/1984
`10/1984
`11/1984
`11/1984
`2/1986
`3/1986
`4/1986
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`10/1986
`9/1987
`2/1988
`5/1988
`7/1989
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`7/1993
`2/1999
`8/1981
`3/1983
`9/1984
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`5/2000
`9/1987
`9/1981
`9/1982
`
`WO
`WO
`WO
`WO
`WO
`WO
`WO
`WO
`WO
`WO
`WO
`ZA
`
`WO 83/00164
`WO 86/01533
`WO 87/02671
`WO 89/00999
`WO 89/01783
`WO 92/16553
`WO 93/07899
`WO 93/10817
`WO 93/21319
`9429351
`WO 97/30087
`8809711
`
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