Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 1 of 175 PageID #:
`4682
`
`(cid:44)(cid:49)(cid:3)(cid:55)(cid:43)(cid:40)(cid:3)(cid:56)(cid:49)(cid:44)(cid:55)(cid:40)(cid:39)(cid:3)(cid:54)(cid:55)(cid:36)(cid:55)(cid:40)(cid:54)(cid:3)(cid:39)(cid:44)(cid:54)(cid:55)(cid:53)(cid:44)(cid:38)(cid:55)(cid:3)(cid:38)(cid:50)(cid:56)(cid:53)(cid:55)(cid:3)
`(cid:41)(cid:50)(cid:53)(cid:3)(cid:55)(cid:43)(cid:40)(cid:3)(cid:39)(cid:44)(cid:54)(cid:55)(cid:53)(cid:44)(cid:38)(cid:55)(cid:3)(cid:50)(cid:41)(cid:3)(cid:39)(cid:40)(cid:47)(cid:36)(cid:58)(cid:36)(cid:53)(cid:40)(cid:3)
`
`(cid:51)(cid:79)(cid:68)(cid:76)(cid:81)(cid:87)(cid:76)(cid:73)(cid:73)(cid:86)(cid:3)(cid:68)(cid:81)(cid:71)(cid:3)(cid:38)(cid:82)(cid:88)(cid:81)(cid:87)(cid:72)(cid:85)(cid:3)(cid:39)(cid:72)(cid:73)(cid:72)(cid:81)(cid:71)(cid:68)(cid:81)(cid:87)(cid:86)(cid:15)(cid:3)
`
`(cid:89)(cid:17)(cid:3)
`
`(cid:38)(cid:40)(cid:47)(cid:47)(cid:55)(cid:53)(cid:44)(cid:50)(cid:49)(cid:15)(cid:3)(cid:44)(cid:49)(cid:38)(cid:17)(cid:15)(cid:3)(cid:38)(cid:40)(cid:47)(cid:47)(cid:55)(cid:53)(cid:44)(cid:50)(cid:49)(cid:15)(cid:3)
`(cid:43)(cid:40)(cid:36)(cid:47)(cid:55)(cid:43)(cid:38)(cid:36)(cid:53)(cid:40)(cid:3)(cid:38)(cid:50)(cid:17)(cid:15)(cid:3)(cid:47)(cid:55)(cid:39)(cid:17)(cid:15)(cid:3)(cid:55)(cid:40)(cid:57)(cid:36)(cid:3)
`(cid:51)(cid:43)(cid:36)(cid:53)(cid:48)(cid:36)(cid:38)(cid:40)(cid:56)(cid:55)(cid:44)(cid:38)(cid:36)(cid:47)(cid:54)(cid:3)(cid:56)(cid:54)(cid:36)(cid:15)(cid:3)(cid:44)(cid:49)(cid:38)(cid:17)(cid:15)(cid:3)(cid:68)(cid:81)(cid:71)(cid:3)
`(cid:55)(cid:40)(cid:57)(cid:36)(cid:3)(cid:51)(cid:43)(cid:36)(cid:53)(cid:48)(cid:36)(cid:38)(cid:40)(cid:56)(cid:55)(cid:44)(cid:38)(cid:36)(cid:47)(cid:54)(cid:3)
`(cid:44)(cid:49)(cid:55)(cid:40)(cid:53)(cid:49)(cid:36)(cid:55)(cid:44)(cid:50)(cid:49)(cid:36)(cid:47)(cid:3)(cid:42)(cid:48)(cid:37)(cid:43)(cid:15)(cid:3)
`
`(cid:38)(cid:17)(cid:36)(cid:17)(cid:3)(cid:49)(cid:82)(cid:17)(cid:3)(cid:20)(cid:27)(cid:16)(cid:28)(cid:24)(cid:16)(cid:38)(cid:41)(cid:38)
`(cid:11)(cid:38)(cid:50)(cid:49)(cid:54)(cid:50)(cid:47)(cid:44)(cid:39)(cid:36)(cid:55)(cid:40)(cid:39)(cid:12)
`
`(cid:42)(cid:40)(cid:49)(cid:40)(cid:49)(cid:55)(cid:40)(cid:38)(cid:43)(cid:15)(cid:3)(cid:44)(cid:49)(cid:38)(cid:17)(cid:15)(cid:3)(cid:38)(cid:44)(cid:55)(cid:60)(cid:3)(cid:50)(cid:41)(cid:3)(cid:43)(cid:50)(cid:51)(cid:40)(cid:15)(cid:3)(cid:68)(cid:81)(cid:71)(cid:3) (cid:12)(cid:3)
`(cid:43)(cid:50)(cid:41)(cid:41)(cid:48)(cid:36)(cid:49)(cid:49)(cid:3)(cid:47)(cid:36)(cid:3)(cid:53)(cid:50)(cid:38)(cid:43)(cid:40)(cid:3)(cid:44)(cid:49)(cid:38)(cid:17)(cid:15)(cid:3)
`(cid:12)(cid:3)
`(cid:12)(cid:3)
`(cid:12)(cid:3)
`(cid:12)(cid:3)
`(cid:12)(cid:3)
`(cid:12)
`(cid:12)(cid:3)
`(cid:12)(cid:3)
`(cid:12)(cid:3)
`(cid:12)(cid:3)
`(cid:12)(cid:3)
`(cid:12)(cid:3)
`(cid:39)(cid:72)(cid:73)(cid:72)(cid:81)(cid:71)(cid:68)(cid:81)(cid:87)(cid:86)(cid:3)(cid:68)(cid:81)(cid:71)(cid:3)(cid:38)(cid:82)(cid:88)(cid:81)(cid:87)(cid:72)(cid:85)(cid:70)(cid:79)(cid:68)(cid:76)(cid:80)(cid:3)(cid:51)(cid:79)(cid:68)(cid:76)(cid:81)(cid:87)(cid:76)(cid:73)(cid:73)(cid:86)(cid:17)(cid:3) (cid:12)(cid:3)
`(cid:12)
`(cid:42)(cid:40)(cid:49)(cid:40)(cid:49)(cid:55)(cid:40)(cid:38)(cid:43)(cid:15)(cid:3)(cid:44)(cid:49)(cid:38)(cid:17)(cid:3)(cid:68)(cid:81)(cid:71)(cid:3)(cid:38)(cid:44)(cid:55)(cid:60)(cid:3)(cid:50)(cid:41)(cid:3)(cid:43)(cid:50)(cid:51)(cid:40)(cid:15)(cid:3)(cid:3) (cid:12)(cid:3)
`(cid:12)(cid:3)
`(cid:51)(cid:79)(cid:68)(cid:76)(cid:81)(cid:87)(cid:76)(cid:73)(cid:73)(cid:86)(cid:3)(cid:68)(cid:81)(cid:71)(cid:3)(cid:38)(cid:82)(cid:88)(cid:81)(cid:87)(cid:72)(cid:85)(cid:70)(cid:79)(cid:68)(cid:76)(cid:80)(cid:3)(cid:39)(cid:72)(cid:73)(cid:72)(cid:81)(cid:71)(cid:68)(cid:81)(cid:87)(cid:86)(cid:15)(cid:3) (cid:12)(cid:3)
`(cid:12)(cid:3)
`(cid:12)(cid:3)
`(cid:12)(cid:3)
`(cid:12)(cid:3)
`(cid:12)(cid:3)
`(cid:12)(cid:3)
`(cid:12)
`(cid:42)(cid:40)(cid:49)(cid:40)(cid:49)(cid:55)(cid:40)(cid:38)(cid:43)(cid:15)(cid:3)(cid:44)(cid:49)(cid:38)(cid:17)(cid:3)(cid:68)(cid:81)(cid:71)(cid:3)(cid:38)(cid:44)(cid:55)(cid:60)(cid:3)(cid:50)(cid:41)(cid:3)(cid:43)(cid:50)(cid:51)(cid:40)(cid:15)(cid:3)(cid:3) (cid:12)(cid:3)
`(cid:12)(cid:3)
`(cid:12)(cid:3)
`(cid:12)(cid:3)
`(cid:12)(cid:3)
`(cid:12)(cid:3)
`(cid:12)(cid:3)
`(cid:12)(cid:3)
`(cid:12)(cid:3)
`(cid:12)(cid:3)
`(cid:45)(cid:50)(cid:44)(cid:49)(cid:55)(cid:3)(cid:38)(cid:47)(cid:36)(cid:44)(cid:48)(cid:3)(cid:38)(cid:50)(cid:49)(cid:54)(cid:55)(cid:53)(cid:56)(cid:38)(cid:55)(cid:44)(cid:50)(cid:49)(cid:3)(cid:38)(cid:43)(cid:36)(cid:53)(cid:55)(cid:3)
`(cid:36)(cid:51)(cid:51)(cid:40)(cid:49)(cid:39)(cid:44)(cid:59)(cid:3)(cid:38)(cid:3)(cid:57)(cid:50)(cid:47)(cid:56)(cid:48)(cid:40)(cid:3)8(cid:3)
`
`(cid:89)(cid:17)(cid:3)
`
`(cid:36)(cid:48)(cid:42)(cid:40)(cid:49)(cid:3)(cid:44)(cid:49)(cid:38)(cid:17)(cid:15)(cid:3)
`
`(cid:39)(cid:72)(cid:73)(cid:72)(cid:81)(cid:71)(cid:68)(cid:81)(cid:87)(cid:3)(cid:68)(cid:81)(cid:71)(cid:3)(cid:38)(cid:82)(cid:88)(cid:81)(cid:87)(cid:72)(cid:85)(cid:70)(cid:79)(cid:68)(cid:76)(cid:80)(cid:3)(cid:51)(cid:79)(cid:68)(cid:76)(cid:81)(cid:87)(cid:76)(cid:73)(cid:73)(cid:17)(cid:3)
`
`(cid:51)(cid:79)(cid:68)(cid:76)(cid:81)(cid:87)(cid:76)(cid:73)(cid:73)(cid:86)(cid:15)(cid:3)
`
`(cid:89)(cid:17)(cid:3)
`
`(cid:54)(cid:36)(cid:48)(cid:54)(cid:56)(cid:49)(cid:42)(cid:3)(cid:37)(cid:44)(cid:50)(cid:40)(cid:51)(cid:44)(cid:54)(cid:3)(cid:38)(cid:50)(cid:17)(cid:15)(cid:3)(cid:47)(cid:55)(cid:39)(cid:15)(cid:3)
`
`(cid:39)(cid:72)(cid:73)(cid:72)(cid:81)(cid:71)(cid:68)(cid:81)(cid:87)(cid:17)(cid:3)
`
`(cid:38)(cid:17)(cid:36)(cid:17)(cid:3)(cid:49)(cid:82)(cid:17)(cid:3)(cid:20)(cid:27)(cid:16)(cid:28)(cid:21)(cid:23)(cid:16)(cid:38)(cid:41)(cid:38)
`
`(cid:38)(cid:17)(cid:36)(cid:17)(cid:3)(cid:49)(cid:82)(cid:17)(cid:3)(cid:20)(cid:27)(cid:16)(cid:20)(cid:22)(cid:25)(cid:22)(cid:16)(cid:38)(cid:41)(cid:38)
`
`
`4682
`
`(cid:44)(cid:49)(cid:3)(cid:55)(cid:43)(cid:40)(cid:3)(cid:56)(cid:49)(cid:44)(cid:55)(cid:40)(cid:39)(cid:3)(cid:54)(cid:55)(cid:36)(cid:55)(cid:40)(cid:54)(cid:3)(cid:39)(cid:44)(cid:54)(cid:55)(cid:53)(cid:44)(cid:38)(cid:55)(cid:3)(cid:38)(cid:50)(cid:56)(cid:53)(cid:55)(cid:3)
`(cid:41)(cid:50)(cid:53)(cid:3)(cid:55)(cid:43)(cid:40)(cid:3)(cid:39)(cid:44)(cid:54)(cid:55)(cid:53)(cid:44)(cid:38)(cid:55)(cid:3)(cid:50)(cid:41)(cid:3)(cid:39)(cid:40)(cid:47)(cid:36)(cid:58)(cid:36)(cid:53)(cid:40)(cid:3)
`
`(cid:51)(cid:79)(cid:68)(cid:76)(cid:81)(cid:87)(cid:76)(cid:73)(cid:73)(cid:86)(cid:3)(cid:68)(cid:81)(cid:71)(cid:3)(cid:38)(cid:82)(cid:88)(cid:81)(cid:87)(cid:72)(cid:85)(cid:3)(cid:39)(cid:72)(cid:73)(cid:72)(cid:81)(cid:71)(cid:68)(cid:81)(cid:87)(cid:86)(cid:15)(cid:3)
`
`(cid:89)(cid:17)(cid:3)
`
`(cid:38)(cid:40)(cid:47)(cid:47)(cid:55)(cid:53)(cid:44)(cid:50)(cid:49)(cid:15)(cid:3)(cid:44)(cid:49)(cid:38)(cid:17)(cid:15)(cid:3)(cid:38)(cid:40)(cid:47)(cid:47)(cid:55)(cid:53)(cid:44)(cid:50)(cid:49)(cid:15)(cid:3)
`(cid:43)(cid:40)(cid:36)(cid:47)(cid:55)(cid:43)(cid:38)(cid:36)(cid:53)(cid:40)(cid:3)(cid:38)(cid:50)(cid:17)(cid:15)(cid:3)(cid:47)(cid:55)(cid:39)(cid:17)(cid:15)(cid:3)(cid:55)(cid:40)(cid:57)(cid:36)(cid:3)
`(cid:51)(cid:43)(cid:36)(cid:53)(cid:48)(cid:36)(cid:38)(cid:40)(cid:56)(cid:55)(cid:44)(cid:38)(cid:36)(cid:47)(cid:54)(cid:3)(cid:56)(cid:54)(cid:36)(cid:15)(cid:3)(cid:44)(cid:49)(cid:38)(cid:17)(cid:15)(cid:3)(cid:68)(cid:81)(cid:71)(cid:3)
`(cid:55)(cid:40)(cid:57)(cid:36)(cid:3)(cid:51)(cid:43)(cid:36)(cid:53)(cid:48)(cid:36)(cid:38)(cid:40)(cid:56)(cid:55)(cid:44)(cid:38)(cid:36)(cid:47)(cid:54)(cid:3)
`(cid:44)(cid:49)(cid:55)(cid:40)(cid:53)(cid:49)(cid:36)(cid:55)(cid:44)(cid:50)(cid:49)(cid:36)(cid:47)(cid:3)(cid:42)(cid:48)(cid:37)(cid:43)(cid:15)(cid:3)
`
`(cid:38)(cid:17)(cid:36)(cid:17)(cid:3)(cid:49)(cid:82)(cid:17)(cid:3)(cid:20)(cid:27)(cid:16)(cid:28)(cid:24)(cid:16)(cid:38)(cid:41)(cid:38)
`(cid:11)(cid:38)(cid:50)(cid:49)(cid:54)(cid:50)(cid:47)(cid:44)(cid:39)(cid:36)(cid:55)(cid:40)(cid:39)(cid:12)
`
`(cid:42)(cid:40)(cid:49)(cid:40)(cid:49)(cid:55)(cid:40)(cid:38)(cid:43)(cid:15)(cid:3)(cid:44)(cid:49)(cid:38)(cid:17)(cid:15)(cid:3)(cid:38)(cid:44)(cid:55)(cid:60)(cid:3)(cid:50)(cid:41)(cid:3)(cid:43)(cid:50)(cid:51)(cid:40)(cid:15)(cid:3)(cid:68)(cid:81)(cid:71)(cid:3) (cid:12)(cid:3)
`(cid:43)(cid:50)(cid:41)(cid:41)(cid:48)(cid:36)(cid:49)(cid:49)(cid:3)(cid:47)(cid:36)(cid:3)(cid:53)(cid:50)(cid:38)(cid:43)(cid:40)(cid:3)(cid:44)(cid:49)(cid:38)(cid:17)(cid:15)(cid:3)
`(cid:12)(cid:3)
`(cid:12)(cid:3)
`(cid:12)(cid:3)
`(cid:12)(cid:3)
`(cid:12)(cid:3)
`(cid:12)
`(cid:12)(cid:3)
`(cid:12)(cid:3)
`(cid:12)(cid:3)
`(cid:12)(cid:3)
`(cid:12)(cid:3)
`(cid:12)(cid:3)
`(cid:39)(cid:72)(cid:73)(cid:72)(cid:81)(cid:71)(cid:68)(cid:81)(cid:87)(cid:86)(cid:3)(cid:68)(cid:81)(cid:71)(cid:3)(cid:38)(cid:82)(cid:88)(cid:81)(cid:87)(cid:72)(cid:85)(cid:70)(cid:79)(cid:68)(cid:76)(cid:80)(cid:3)(cid:51)(cid:79)(cid:68)(cid:76)(cid:81)(cid:87)(cid:76)(cid:73)(cid:73)(cid:86)(cid:17)(cid:3) (cid:12)(cid:3)
`(cid:12)
`(cid:42)(cid:40)(cid:49)(cid:40)(cid:49)(cid:55)(cid:40)(cid:38)(cid:43)(cid:15)(cid:3)(cid:44)(cid:49)(cid:38)(cid:17)(cid:3)(cid:68)(cid:81)(cid:71)(cid:3)(cid:38)(cid:44)(cid:55)(cid:60)(cid:3)(cid:50)(cid:41)(cid:3)(cid:43)(cid:50)(cid:51)(cid:40)(cid:15)(cid:3)(cid:3) (cid:12)(cid:3)
`(cid:12)(cid:3)
`(cid:51)(cid:79)(cid:68)(cid:76)(cid:81)(cid:87)(cid:76)(cid:73)(cid:73)(cid:86)(cid:3)(cid:68)(cid:81)(cid:71)(cid:3)(cid:38)(cid:82)(cid:88)(cid:81)(cid:87)(cid:72)(cid:85)(cid:70)(cid:79)(cid:68)(cid:76)(cid:80)(cid:3)(cid:39)(cid:72)(cid:73)(cid:72)(cid:81)(cid:71)(cid:68)(cid:81)(cid:87)(cid:86)(cid:15)(cid:3) (cid:12)(cid:3)
`(cid:12)(cid:3)
`(cid:12)(cid:3)
`(cid:12)(cid:3)
`(cid:12)(cid:3)
`(cid:12)(cid:3)
`(cid:12)(cid:3)
`(cid:12)
`(cid:42)(cid:40)(cid:49)(cid:40)(cid:49)(cid:55)(cid:40)(cid:38)(cid:43)(cid:15)(cid:3)(cid:44)(cid:49)(cid:38)(cid:17)(cid:3)(cid:68)(cid:81)(cid:71)(cid:3)(cid:38)(cid:44)(cid:55)(cid:60)(cid:3)(cid:50)(cid:41)(cid:3)(cid:43)(cid:50)(cid:51)(cid:40)(cid:15)(cid:3)(cid:3) (cid:12)(cid:3)
`(cid:12)(cid:3)
`(cid:12)(cid:3)
`(cid:12)(cid:3)
`(cid:12)(cid:3)
`(cid:12)(cid:3)
`(cid:12)(cid:3)
`(cid:12)(cid:3)
`(cid:12)(cid:3)
`(cid:12)(cid:3)
`(cid:45)(cid:50)(cid:44)(cid:49)(cid:55)(cid:3)(cid:38)(cid:47)(cid:36)(cid:44)(cid:48)(cid:3)(cid:38)(cid:50)(cid:49)(cid:54)(cid:55)(cid:53)(cid:56)(cid:38)(cid:55)(cid:44)(cid:50)(cid:49)(cid:3)(cid:38)(cid:43)(cid:36)(cid:53)(cid:55)(cid:3)
`(cid:36)(cid:51)(cid:51)(cid:40)(cid:49)(cid:39)(cid:44)(cid:59)(cid:3)(cid:38)(cid:3)(cid:57)(cid:50)(cid:47)(cid:56)(cid:48)(cid:40)(cid:3)8(cid:3)
`
`(cid:89)(cid:17)(cid:3)
`
`(cid:36)(cid:48)(cid:42)(cid:40)(cid:49)(cid:3)(cid:44)(cid:49)(cid:38)(cid:17)(cid:15)(cid:3)
`
`(cid:39)(cid:72)(cid:73)(cid:72)(cid:81)(cid:71)(cid:68)(cid:81)(cid:87)(cid:3)(cid:68)(cid:81)(cid:71)(cid:3)(cid:38)(cid:82)(cid:88)(cid:81)(cid:87)(cid:72)(cid:85)(cid:70)(cid:79)(cid:68)(cid:76)(cid:80)(cid:3)(cid:51)(cid:79)(cid:68)(cid:76)(cid:81)(cid:87)(cid:76)(cid:73)(cid:73)(cid:17)(cid:3)
`
`(cid:51)(cid:79)(cid:68)(cid:76)(cid:81)(cid:87)(cid:76)(cid:73)(cid:73)(cid:86)(cid:15)(cid:3)
`
`(cid:89)(cid:17)(cid:3)
`
`(cid:54)(cid:36)(cid:48)(cid:54)(cid:56)(cid:49)(cid:42)(cid:3)(cid:37)(cid:44)(cid:50)(cid:40)(cid:51)(cid:44)(cid:54)(cid:3)(cid:38)(cid:50)(cid:17)(cid:15)(cid:3)(cid:47)(cid:55)(cid:39)(cid:15)(cid:3)
`
`(cid:39)(cid:72)(cid:73)(cid:72)(cid:81)(cid:71)(cid:68)(cid:81)(cid:87)(cid:17)(cid:3)
`
`(cid:38)(cid:17)(cid:36)(cid:17)(cid:3)(cid:49)(cid:82)(cid:17)(cid:3)(cid:20)(cid:27)(cid:16)(cid:28)(cid:21)(cid:23)(cid:16)(cid:38)(cid:41)(cid:38)
`
`(cid:38)(cid:17)(cid:36)(cid:17)(cid:3)(cid:49)(cid:82)(cid:17)(cid:3)(cid:20)(cid:27)(cid:16)(cid:20)(cid:22)(cid:25)(cid:22)(cid:16)(cid:38)(cid:41)(cid:38)
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 2 of 175 PageID #:
`4683
`
`JA00003124
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 3 of 175 PageID #:
`4684
`
`JA00003125
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 4 of 175 PageID #:
`4685
`
`JA00003126
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 5 of 175 PageID #:
`4686
`
`JA00003127
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 6 of 175 PageID #:
`4687
`
`JA00003128
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 7 of 175 PageID #:
`4688
`
`JA00003129
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 8 of 175 PageID #:
`4689
`
`JA00003130
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 9 of 175 PageID #:
`4690
`
`JA00003131
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 10 of 175 PageID #:
`4691
`
`JA00003132
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 11 of 175 PageID #:
`4692
`
`JA00003133
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 12 of 175 PageID #:
`4693
`
`JA00003134
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 13 of 175 PageID #:
`4694
`
`JA00003135
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 14 of 175 PageID #:
`4695
`
`JA00003136
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 15 of 175 PageID #:
`4696
`
`JA00003137
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 16 of 175 PageID #:
`4697
`
`JA00003138
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 17 of 175 PageID #:
`4698
`
`JA00003139
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 18 of 175 PageID #:
`4699
`
`JA00003140
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 19 of 175 PageID #:
`4700
`
`JA00003141
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 20 of 175 PageID #:
`4701
`
`JA00003142
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 21 of 175 PageID #:
`4702
`
`JA00003143
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 22 of 175 PageID #:
`4703
`
`JA00003144
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 23 of 175 PageID #:
`4704
`
`JA00003145
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 24 of 175 PageID #:
`4705
`
`JA00003146
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 25 of 175 PageID #:
`4706
`
`JA00003147
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 26 of 175 PageID #:
`4707
`
`JA00003148
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 27 of 175 PageID #:
`4708
`
`JA00003149
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 28 of 175 PageID #:
`4709
`
`JA00003150
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 29 of 175 PageID #:
`4710
`
`JA00003151
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 30 of 175 PageID #:
`4711
`
`JA00003152
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 31 of 175 PageID #:
`4712
`
`JA00003153
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 32 of 175 PageID #:
`4713
`
`JA00003154
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 33 of 175 PageID #:
`4714
`
`JA00003155
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 34 of 175 PageID #:
`4715
`
`JA00003156
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 35 of 175 PageID #:
`4716
`
`JA00003157
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 36 of 175 PageID #:
`4717
`
`JA00003158
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 37 of 175 PageID #:
`4718
`
`JA00003159
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 38 of 175 PageID #:
`4719
`
`JA00003160
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 39 of 175 PageID #:
`4720
`
`JA00003161
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 40 of 175 PageID #:
`4721
`
`JA00003162
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 41 of 175 PageID #:
`4722
`
`JA00003163
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 42 of 175 PageID #:
`4723
`
`JA00003164
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 43 of 175 PageID #:
`4724
`
`JA00003165
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 44 of 175 PageID #:
`4725
`
`JA00003166
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 45 of 175 PageID #:
`4726
`
`JA00003167
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 46 of 175 PageID #:
`4727
`
`JA00003168
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 47 of 175 PageID #:
`4728
`
`JA00003169
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 48 of 175 PageID #:
`4729
`
`JA00003170
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 49 of 175 PageID #:
`4730
`
`JA00003171
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 50 of 175 PageID #:
`4731
`
`JA00003172
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 51 of 175 PageID #:
`4732
`
`JA00003173
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 52 of 175 PageID #:
`4733
`
`JA00003174
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 53 of 175 PageID #:
`4734
`
`JA00003175
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 54 of 175 PageID #:
`4735
`
`JA00003176
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 55 of 175 PageID #:
`4736
`
`JA00003177
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 56 of 175 PageID #:
`4737
`
`JA00003178
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 57 of 175 PageID #:
`4738
`
`JA00003179
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 58 of 175 PageID #:
`4739
`
`JA00003180
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 59 of 175 PageID #:
`4740
`
`JA00003181
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 60 of 175 PageID #:
`4741
`
`JA00003182
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 61 of 175 PageID #:
`4742
`
`JA00003183
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 62 of 175 PageID #:
`4743
`
`JA00003184
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 63 of 175 PageID #:
`4744
`
`JA00003185
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 64 of 175 PageID #:
`4745
`
`JA00003186
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 65 of 175 PageID #:
`4746
`
`JA00003187
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 66 of 175 PageID #:
`4747
`
`JA00003188
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 67 of 175 PageID #:
`4748
`
`JA00003189
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 68 of 175 PageID #:
`4749
`
`JA00003190
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 69 of 175 PageID #:
`4750
`
`JA00003191
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 70 of 175 PageID #:
`4751
`
`JA00003192
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 71 of 175 PageID #:
`4752
`
`JA00003193
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 72 of 175 PageID #:
`4753
`
`JA00003194
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 73 of 175 PageID #:
`4754
`
`JA00003195
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 74 of 175 PageID #:
`4755
`
`JA00003196
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 75 of 175 PageID #:
`4756
`
`JA00003197
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 76 of 175 PageID #:
`4757
`
`JA00003198
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 77 of 175 PageID #:
`4758
`
`JA00003199
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 78 of 175 PageID #:
`4759
`
`JA00003200
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 79 of 175 PageID #:
`4760
`
`JA00003201
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 80 of 175 PageID #:
`4761
`
`JA00003202
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 81 of 175 PageID #:
`4762
`
`JA00003203
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 82 of 175 PageID #:
`4763
`
`JA00003204
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 83 of 175 PageID #:
`4764
`
`JA00003205
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 84 of 175 PageID #:
`4765
`
`JA00003206
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 85 of 175 PageID #:
`4766
`
`JA00003207
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 86 of 175 PageID #:
`4767
`
`JA00003208
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 87 of 175 PageID #:
`4768
`
`JA00003209
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 88 of 175 PageID #:
`4769
`
`JA00003210
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 89 of 175 PageID #:
`4770
`
`JA00003211
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 90 of 175 PageID #:
`4771
`
`JA00003212
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 91 of 175 PageID #:
`4772
`
`JA00003213
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 92 of 175 PageID #:
`4773
`
`JA00003214
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 93 of 175 PageID #:
`4774
`
`JA00003215
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 94 of 175 PageID #:
`4775
`
`JA00003216
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 95 of 175 PageID #:
`4776
`
`JA00003217
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 96 of 175 PageID #:
`4777
`
`JA00003218
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 97 of 175 PageID #:
`4778
`
`JA00003219
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 98 of 175 PageID #:
`4779
`
`JA00003220
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 99 of 175 PageID #:
`4780
`
`JA00003221
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 100 of 175 PageID #:
`4781
`
`JA00003222
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 101 of 175 PageID #:
`4782
`
`JA00003223
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 102 of 175 PageID #:
`4783
`
`JA00003224
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 103 of 175 PageID #:
`4784
`
`JA00003225
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 104 of 175 PageID #:
`4785
`
`JA00003226
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 105 of 175 PageID #:
`4786
`
`JA00003227
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 106 of 175 PageID #:
`4787
`
`JA00003228
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 107 of 175 PageID #:
`4788
`
`JA00003229
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 108 of 175 PageID #:
`4789
`
`JA00003230
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 109 of 175 PageID #:
`4790
`
`JA00003231
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 110 of 175 PageID #:
`4791
`
`JA00003232
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 111 of 175 PageID #:
`4792
`
`JA00003233
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 112 of 175 PageID #:
`4793
`
`JA00003234
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 113 of 175 PageID #:
`4794
`
`JA00003235
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 114 of 175 PageID #:
`4795
`
`JA00003236
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 115 of 175 PageID #:
`4796
`
`JA00003237
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 116 of 175 PageID #:
`4797
`
`JA00003238
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 117 of 175 PageID #:
`4798
`
`JA00003239
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 118 of 175 PageID #:
`4799
`
`JA00003240
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 119 of 175 PageID #:
`4800
`
`JA00003241
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 120 of 175 PageID #:
`4801
`
`JA00003242
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 121 of 175 PageID #:
`4802
`
`JA00003243
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 122 of 175 PageID #:
`4803
`
`JA00003244
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 123 of 175 PageID #:
`4804
`
`JA00003245
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 124 of 175 PageID #:
`4805
`
`JA00003246
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 125 of 175 PageID #:
`4806
`
`JA00003247
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 126 of 175 PageID #:
`4807
`
`JA00003248
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 127 of 175 PageID #:
`4808
`
`JA00003249
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 128 of 175 PageID #:
`4809
`
`JA00003250
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 129 of 175 PageID #:
`4810
`
`JA00003251
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 130 of 175 PageID #:
`4811
`
`JA00003252
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 131 of 175 PageID #:
`4812
`
`JA00003253
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 132 of 175 PageID #:
`4813
`
`JA00003254
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 133 of 175 PageID #:
`4814
`
`JA00003255
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 134 of 175 PageID #:
`4815
`
`JA00003256
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 135 of 175 PageID #:
`4816
`
`JA00003257
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 136 of 175 PageID #:
`4817
`
`JA00003258
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 137 of 175 PageID #:
`4818
`
`JA00003259
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 138 of 175 PageID #:
`4819
`
`JA00003260
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 139 of 175 PageID #:
`4820
`
`JA00003261
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 140 of 175 PageID #:
`4821
`
`JA00003262
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 141 of 175 PageID #:
`4822
`
`JA00003263
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 142 of 175 PageID #:
`4823
`
`JA00003264
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 143 of 175 PageID #:
`4824
`
`JA00003265
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 144 of 175 PageID #:
`4825
`
`JA00003266
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 145 of 175 PageID #:
`4826
`
`JA00003267
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 146 of 175 PageID #:
`4827
`
`JA00003268
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 147 of 175 PageID #:
`4828
`
`JA00003269
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 148 of 175 PageID #:
`4829
`
`JA00003270
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 149 of 175 PageID #:
`4830
`
`JA00003271
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 150 of 175 PageID #:
`4831
`
`JA00003272
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 151 of 175 PageID #:
`4832
`
`JA00003273
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 152 of 175 PageID #:
`4833
`
`JA00003274
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 153 of 175 PageID #:
`4834
`
`JA00003275
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 154 of 175 PageID #:
`4835
`
`JA00003276
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 155 of 175 PageID #:
`4836
`
`JA00003277
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 156 of 175 PageID #:
`4837
`
`JA00003278
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 157 of 175 PageID #:
`4838
`
`JA00003279
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 158 of 175 PageID #:
`4839
`
`JA00003280
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 159 of 175 PageID #:
`4840
`
`JA00003281
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 160 of 175 PageID #:
`4841
`
`JA00003282
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 161 of 175 PageID #:
`4842
`
`JA00003283
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 162 of 175 PageID #:
`4843
`
`JA00003284
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 163 of 175 PageID #:
`4844
`
`JA00003285
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 164 of 175 PageID #:
`4845
`
`JA00003286
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 165 of 175 PageID #:
`4846
`
`JA00003287
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 166 of 175 PageID #:
`4847
`
`JA00003288
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 167 of 175 PageID #:
`4848
`
`JA00003289
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 168 of 175 PageID #:
`4849
`
`JA00003290
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 169 of 175 PageID #:
`4850
`
`JA00003291
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 170 of 175 PageID #:
`4851
`
`JA00003292
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 171 of 175 PageID #:
`4852
`
`An Improved Heat-stable Glutamine-f ree Chemically
`Defined Medium for Growth of Mammalian Cells
`
`STANLEY C. NAGLE, JR. AND BRUCE L. BROWN
`Biological Sciences Laboratories, U. S . A m y Biological Defense Research
`Center, Fort Detrick, Frederick, Maryland 21 701
`
`A heat-stable chemically defined medium, free of glutamine, is
`ABSTRACT
`described for the growth of mammalian cells in suspension culture. The presence
`of L-alanine in the defined medium permitted the omission of glutamine. A 22-
`fold increase in the population of a substrain of mouse L cells was obtained
`(3.4 X lo6 cells/ml) in six days with no medium replenishment during incuba-
`tion. Maximum yields (27 X 10' cells/ml) were obtained by daily medium re-
`placement and venting of cultures. Growth was also improved in a line of cat
`kidney cells and HeLa cells, and in another substrain of L cells.
`
`A heat-stable chemically defined medium
`reported earlier (Nagle, '68) was im-
`proved by increasing the choline level
`(Nagle, '69). Glutamine was a neces-
`sary component of both media, but it
`had to be separately sterilized. An auto-
`clavable medium described by Yamane et
`al. ('68) also required the separate sterili-
`zation of glutamine. The present paper de-
`scribes improvements made to the heat-
`stable medium that have allowed omission
`of glutamine and have resulted in higher
`cell yields.
`
`MATERIALS AND METHODS
`Media
`All ingredients except methylcellulose
`and sodium bicarbonate necessary for a
`total volume of 1000 ml of the chemically
`defined medium (table 1 ) were dissolved
`in 970 ml of hot distilled water. Ten milli-
`liter of x 100 vitamin solution were
`added from storage at -25°C then, 245 ml
`of the solution were dispensed into 16-02
`prescription bottles containing 125 mg of
`dry methylcellulose. The bottles were auto-
`claved 15 min at 121 "C with loosened caps.
`After cooling, the caps were tightened and
`the bottles were stored at 5°C. Prior to use,
`5 ml of sterile 5% sodium bicarbonate
`solution were added to each bottle, the caps
`were tightened to prevent loss of COZ, and
`the medium was stored at 5°C. The bicar-
`bonate solution had been sterilized by auto-
`claving in nearly filled, tightly stoppered
`
`J. CELL. PHYSIOL., 77: 259-264.
`
`50-ml serum bottles. At time of inoculation,
`an additional 0.7 ml of bicarbonate solu-
`tion was added to each 25 ml of medium
`contained in 100-ml serum bottle culture
`flasks fitted with rubber stoppers. When
`the cultures were vented, stoppers were re-
`placed with loose-fitting caps from steri-
`lized 2-oz prescription bottles and smaller
`amounts of bicarbonate (depending on cell
`population) were added to maintain a pH
`of 7.2 to 7.4. When antibiotics were used
`in experimental media, sterile solutions
`( X 100) stored at -25°C were thawed and
`added aseptically to give final concentra-
`tions of 100 pg/ml of streptomycin and
`100 units/ml of penicillin.
`Incubation and cell enumeration pro-
`cedures were presented previously (Nagle,
`'68). Three of the cell lines used, LSN ( a
`substrain of mouse L-929 cells) HeLa, and
`cat kidney (CK) cells were described previ-
`ously (Nagle, '68) and had been growing
`in suspension in chemically defined media
`for five to eight years prior to the present
`study. The LDR cell line (another substrain
`of mouse L-929 cells), supplied by W. F.
`Daniels of Fort Detrick, was described by
`Higuchi ('70) and had been growing for
`approximately one year in chemically de-
`fined media.
`
`RESULTS
`The composition of the medium devel-
`oped in these studies is given in table 1.
`Received Aug. 18, '70. Accepted Oct. 22, '70.
`
`259
`
`JA00003293
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 172 of 175 PageID #:
`4853
`260
`STANLEY C. NAGLE, JR. AND BRUCE L. BROWN
`
`TABLE 1
`Heat-stable, glutamine-free chemically defined medium
`
`Component
`
`Amino acids
`L-Alanine
`L- Arginine -HC1
`L-Asparagine
`L-Cysteine. HC1
`L-Glutamic Acid
`L-Histidine. HC1
`L-Isoleucine
`L-Leucine
`Ltysine
`L-Methionine
`L-Phenylalanine
`L-Proline
`L-Serine
`L-Threonine
`L-Tryptophan
`L-Tyrosine
`L-Valine
`Carbon sources
`Glucose
`Sodium pyruvate
`
`Conc.
`ms/L
`
`400
`100
`300
`75
`150
`60
`150
`300
`300
`60
`120
`300
`300
`135
`60
`120
`150
`
`3000
`110
`
`Salts, etc.
`NaCl
`KC1
`CaC12.2HzO
`Ferric ammonium citrate
`MgCL. 6Hz0
`NaH2P04- H20
`ZnSOl. 7Hz0
`Phenol red
`Methylcellulose, 15 cps
`NaHCOa
`Vitamins
`D-Biotin
`Choline . C1
`Folic acid
`Niacinamide
`Calcium pantothenate
`Pyridoxal- HC1
`Thiamine .HCl
`idnositol
`Riboflavin
`Biz
`1 Added aseptically as 5% solution.
`2 Added as a x 100 solution during medium preparation.
`
`Component
`
`Conc.
`~~~ mg/L
`
`~
`
`7400
`400
`265
`3
`275
`300
`0.3
`10
`500
`1000 1
`1
`50
`1
`1
`2
`1
`1
`1
`0.1
`0.002
`
`TABLE 2
`Growth of LDR cells in heat-stable medium with no medium replenishment
`
`Omission from medium
`
`None (medium as table 1)
`Alanine
`Asparagine
`Glutamic acid
`Proline
`Serine
`Ala, asp, glutamate, prol, ser
`Fe
`Zn
`NaHZP04,HzO reduced to 150 mg/L
`1 Zero-day count was 0.22 x 10~/ml.
`
`Growth 1
`(No. cells x 106/ml)
`6 days
`2.90
`0.47
`2.61
`1.52
`2.13
`0.21
`0.12
`1.91
`1.69
`2.05
`
`5 days
`1.63
`0.37
`1.42
`0.95
`1.38
`0.45
`0.13
`1.43
`1.36
`1.38
`
`7 days
`2.76
`0.49
`2.48
`2.47
`2.56
`0.12
`0.12
`2.38
`2.46
`2.43
`
`The medium differs from that previously
`reported (NagIe, '69) by ( 1 ) the omission
`of glutamine, (2) the addition of L-alanine,
`L-asparagine, L-glutamic acid, L-proline,
`L-serine, iron and zinc, and
`( 3 ) an
`increase in the concentrations of glucose
`and NaH2P04.H20 from 1000 mg and 100
`mg per liter respectively.
`A comparison of the effectiveness of the
`medium and the effect of the variation of
`composition on the growth of LDR cells is
`given in table 2. Alanine and serine were
`essential for LDR cells, but evidence for
`improved cell growth with asparagine and
`
`proline was equivocal. Similar results were
`obtained with LSN cells with the exception
`that serine was not essential. Asparagine
`and proline were included because many
`experiments with LSN cells demonstrated
`that these amino acids reduced the lag
`phase on transfer of cells from glutamine-
`containing to glutamine-free media. In the
`presence of asparagine and proline, a lag
`in growth of one or two days was occa-
`sionally noted when LDR cells from
`medium containing glutamine were inocu-
`lated into glutamine-free medium. Glu-
`tamic acid was stimulatory in the absence
`
`JA00003294
`
`
`
`Case 1:18-cv-01363-CFC Document 48-9 Filed 12/11/18 Page 173 of 175 PageID #:
`4854
`AUTOCLAVABLE MEDIUM FOR ANIMAL CELLS
`
`26 1
`
`TABLE 3
`Growth of various cell lines in the glutamine-free heat-stable medium
`with no medium replenishment
`- - - . . __
`Gmwth
`(No. cells x lW/ml)
`Final growth
`0 Days
`0.21
`3.08
`1.27
`0.25
`3.36
`0.15
`2.04
`0.19
`
`Days of
`incubation
`7
`7
`6
`6
`
`Cell
`
`CK
`HeLa
`LDR
`LSN
`
`Fold
`increase
`14.7
`5.1
`22.4
`10.7
`
`TABLE 4
`Grawth of cells in heat-stable medium with
`medium replenishments
`
`Cell
`
`Maximum growth
`(No. cells x lOa/ml)
`Improved
`Glutamine 1
`glutamine-free
`medium
`medium
`3.4
`28.5
`CK
`3.4
`4.0
`HeLa
`27.3
`02
`LDR
`LSN
`4.7
`10.5
`1 Data were reported previously (Nagle, '68).
`2LDR cells did not grow in this medium because
`serine is not present.
`
`of glutamine. The phosphate and glucose
`concentrations were increased to insure
`sufficient levels for higher cell populations.
`Iron and zinc were added as a result of
`studies by Thomas and Johnson ('67) and
`Higuchi ('70).
`The data in table 3 compare the growth
`of four cell lines without replenishment of
`medium. Table 4 shows the maximum
`growth obtained when media were changed
`daily and cultures were vented after popu-
`lations reached two to three million cells
`per milliliter. Data previously reported for
`cells grown in the heat-stable glutamine
`medium (Nagle, '68) are given for
Accessing this document will incur an additional charge of $.
After purchase, you can access this document again without charge.
Accept $ Charge
Still Working On It
This document is taking longer than usual to download. This can happen if we need to contact the court directly to obtain the document and their servers are running slowly.
Give it another minute or two to complete, and then try the refresh button.
A few More Minutes ... Still Working
It can take up to 5 minutes for us to download a document if the court servers are running slowly.
Thank you for your continued patience.
This document could not be displayed.
We could not find this document within its docket. Please go back to the docket page and check the link. If that does not work, go back to the docket and refresh it to pull the newest information.
Your account does not support viewing this document.
You need a Paid Account to view this document. Click here to change your account type.
Your account does not support viewing this document.
Set your membership
status to view this document.
With a Docket Alarm membership, you'll
get a whole lot more, including:
- Up-to-date information for this case.
- Email alerts whenever there is an update.
- Full text search for other cases.
- Get email alerts whenever a new case matches your search.
One Moment Please
The filing “” is large (MB) and is being downloaded.
Please refresh this page in a few minutes to see if the filing has been downloaded. The filing will also be emailed to you when the download completes.
Your document is on its way!
If you do not receive the document in five minutes, contact support at support@docketalarm.com.
Sealed Document
We are unable to display this document, it may be under a court ordered seal.
If you have proper credentials to access the file, you may proceed directly to the court's system using your government issued username and password.
Access Government Site
