Case 1:20-cv-01580-LPS Document 9-6 Filed 12/10/20 Page 1 of 58 PageID #: 982
`Case 1:20-cv-01580-LPS Document 9—6 Filed 12/10/20 Page 1 of 58 PageID #: 982
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`EXHIBIT 6
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`EXHIBIT 6
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`

`

`( 12 ) United States Patent
`Talasaz et al .
`
`( * ) Notice :
`
`( 54 ) SYSTEMS AND METHODS TO DETECT
`RARE MUTATIONS AND COPY NUMBER
`VARIATION
`( 71 ) Applicant : Guardant Health , Inc . , Redwood City ,
`CA ( US )
`( 72 ) Inventors : Amir Ali Talasaz , Menlo Park , CA
`( US ) ; Helmy Eltoukhy , Atherton , CA
`( US )
`( 73 ) Assignee : Guardant Helath , Inc . , Redwood City ,
`CA ( US )
`Subject to any disclaimer , the term of this
`patent is extended or adjusted under 35
`U . S . C . 154 ( b ) by 0 days .
`This patent is subject to a terminal dis
`claimer .
`( 21 ) Appl . No . : 15 / 076 , 565
`( 22 ) Filed :
`Mar . 21 , 2016
`Prior Publication Data
`( 65 )
`US 2016 / 0251704 A1 Sep . 1 , 2016
`Related U . S . Application Data
`( 63 ) Continuation of application No . 14 / 855 , 301 , filed on
`Sep . 15 , 2015 , which is a continuation of application
`No . PCT / US2014 / 000048 , filed on Mar . 15 , 2014 ,
`which is a continuation - in - part of application No .
`PCT / US2013 / 058061 , filed on Sep . 4 , 2013 , said
`application No .
`PCT / US2014 / 000048
`is
`a
`continuation - in - part of application No . 13 / 969 , 260 ,
`filed on Aug . 16 , 2013 , now abandoned .
`( 60 ) Provisional application No . 61 / 793 , 997 , filed on Mar .
`15 , 2013 , provisional application No . 61 / 845 , 987 ,
`filed on Jul . 13 , 2013 , provisional application No .
`61 / 948 , 530 , filed on Mar . 5 , 2014 , provisional
`application No . 61 / 696 , 734 , filed on Sep . 4 , 2012 ,
`provisional application No . 61 / 704 , 400 , filed on Sep .
`21 , 2012 , provisional application No . 61 / 793 , 997 ,
`filed on Mar . 15 , 2013 , provisional application No .
`61 / 845 , 987 ,
`filed on Jul . 13 , 2013 , provisional
`application No . 61 / 696 , 734 , filed on Sep . 4 , 2012 ,
`provisional application No . 61 / 704 , 400 , filed on Sep .
`21 , 2012 , provisional application No . 61 / 793 , 997 ,
`filed on Mar . 15 , 2013 .
`Int . CI .
`C12P 19 / 34
`C120 1 / 68
`G06F 19 / 22
`U . S . CI .
`CPC . . . . . . . . . C12Q 1 / 6827 ( 2013 . 01 ) ; C12Q 1 / 6806
`( 2013 . 01 ) ; GO6F 19 / 22 ( 2013 . 01 ) ; C12Q
`1 / 6869 ( 2013 . 01 )
`( 58 ) Field of Classification Search
`C12Q 1 / 6806 ; C12Q 2521 / 501 ; C12Q
`2525 / 191 ; C12Q 2565 / 514 ; C12Q 1 / 6827 ;
`C12Q 1 / 6869 ; GO6F 19 / 22
`
`Case 1:20-cv-01580-LPS Document 9-6 Filed 12/10/20 Page 2 of 58 PageID #: 983
`
`( 51 )
`
`( 52 )
`
`( 2006 . 01 )
`( 2018 . 01 )
`( 2011 . 01 )
`
`US009902992B2
`
`( 10 ) Patent No . :
`( 45 ) Date of Patent :
`
`US 9 , 902 , 992 B2
`* Feb . 27 , 2018
`
`USPC . . . . . . . . . . . . . . . . . . .
`. . . . . . . 435 / 91 . 2
`See application file for complete search history .
`
`( 56 )
`
`CN
`EP
`
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`OTHER PUBLICATIONS
`Alkan , et al . Personalized copy number and segmental duplication
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`( Continued )
`
`Primary Examiner - Cynthia B Wilder
`( 74 ) Attorney , Agent , or Firm — Wilson Sonsini Goodrich
`& Rosati
`
`ABSTRACT
`( 57 )
`The present disclosure provides a system and method for the
`detection of rare mutations and copy number variations in
`cell free polynucleotides . Generally , the systems and meth
`ods comprise sample preparation , or the extraction and
`isolation of cell free polynucleotide sequences from a bodily
`fluid ; subsequent sequencing of cell free polynucleotides by
`techniques known in the art ; and application of bioinfor
`matics tools to detect rare mutations and copy number
`variations as compared to a reference . The systems and
`methods also may contain a database or collection of dif
`ferent rare mutations or copy number variation profiles of
`different diseases , to be used as additional references in
`aiding detection of rare mutations , copy number variation
`profiling or general genetic profiling of a disease .
`
`33 Claims , 16 Drawing Sheets
`
`

`

`( 56 )
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