Chromatography Products
`from Polymer Laboratories
`
`GPC/SEC Columns & Standards
`
`Instrumentation & Software for
`Polymer & Materials Characterization
`
`Evaporative Light
`Scattering Detectors
`
`Polymeric HPLC Columns & Media
`
`www.polymerlabs.com
`
`ISS U E
`
`3
`
`
`
`2 0 0 4 - 2 0 0 5
`
`
`
`1
`
`MTX1024
`
`

`

`Polymer Laboratories' Chromatography Products n Specialist Polymer Particles
`
`Polymer Laboratories - A Customer Orientated Company
`
`This catalog has been designed as an easy
`to use guide to Polymer Laboratories' range
`of chromatographic columns, standards, media,
`instrumentation and software.
`
`For further information, please visit our website
`at www.polymerlabs.com for product news and
`specifications, company news, PL's Distributor
`directory, forthcoming seminars, job vacancies,
`etc.
`
`Contacting Polymer Laboratories
`
`North America
`Polymer Laboratories Inc
`Amherst Fields Research Park, 160 Old Farm Road
`Amherst, MA 01002, USA
`Tel, toll free 800 767 3963 or tel 413 253 9554
`Fax 413 253 2476
`Email SalesUS@polymerlabs.com
`Email SupportUS@polymerlabs.com
`
`ONLINE
`ONLINE
`SHOPPING
`SHOPPING
`FOR NORTH AMERICA
`FOR NORTH AMERICA
`www.polymerlabs.com
`www.polymerlabs.com
`
`Credit Cards Accepted
`
`State of the Art Chemical Engineering
`To meet customer demand, Polymer Laboratories opened
`its new, state of the art chemical engineering production
`plant in 2002, and established its place as one of the
`world's largest suppliers of high performance polymeric
`particles. This ensures consistent, reliable supply of top
`quality chromatographic products to our customers.
`
`UK / International
`Polymer Laboratories Ltd
`Essex Road, Church Stretton, Shropshire SY6 6AX, UK
`Tel (+44) 01694 723581
`Fax (+44) 01694 722171
`Email Sales@polymerlabs.com
`Email Support@polymerlabs.com
`
`ISO 9001:2000 Standard
`Polymer Laboratories has recently been recognized as a
`customer-focussed company by the receipt of the
`enhanced customer satisfaction standard, ISO 9001:2000
`Standard.
`
`Credit Cards Accepted
`
`Contact the UK office for details of your local distributor
`
`Benelux
`Polymer Laboratories B.V.
`Sourethweg 1, 6422 PC Heerlen, The Netherlands
`Tel (+31) 045 5414748
`Fax (+31) 045 5410005
`Email PLBenelux@polymerlabs.com
`
`Germany
`Polymer Laboratories GmbH
`PEKA Park T5 (001), Otto-Hesse-Straße 19
`D-64293 Darmstadt, Germany
`Tel (+49) 06151 860690
`Fax (+49) 06151 860670
`Email PLDeutsch@polymerlabs.com
`
`France
`Polymer Laboratories SARL
`Parc d’affaires Marseille Sud, Impasse du Paradou Bâtiment A4
`BP 159, 13276 Marseille Cedex 09, France
`Tel (+33) 04 91 17 64 00
`Fax (+33) 04 91 17 64 01
`Email Support@polymerlabs.fr
`Website www.polymerlabs.fr
`
`www.polymerlabs.com
`
`2
`
`■
`

`

`Contents
`
`GPC/SEC Columns & Standards
`
`Organic GPC Columns - PlusPore, PLgel, PL EnviroPrep, PL HFIPgel
`
`Aqueous SEC Columns - PL aquagel-OH
`
`Rapid GPC/SEC Columns - PL Rapide
`
`Polymer Standards
`
`Specialty Polymers
`
`Instrumentation
`
`Integrated GPC Systems - PL-GPC 220, PL-GPC 120, PL-XT 220, PL-GPC 50
`
`Specialist GPC Detectors - PL-BV 400, PL GPC-FTIR Interface
`
`GPC Sample Handling Systems - PL-SP 260VS, PL-XTR
`
`GPC/Multi Detector Software - Cirrus™
`
`LC Software - Stratos
`
`Online Polymerization Monitoring & Control - PL-PMC Systems
`
`Particle Size Distribution Analysis - PL-PSDA
`
`Evaporative Light Scattering Detectors -
`PL-ELS 2100, PL-ELS 1000, PL-ELS 1000µ
`
`PL Network Interface
`
`HPLC Columns & Media
`
`Polymeric Columns for Pharmacopeia Methods
`
`Capillary & Micro Chromatography Columns
`
`Analytical Reversed Phase Columns - PLRP-S
`
`Analytical Ion Exchange Columns - PL-SAX, PL-SCX
`
`BioProcess Chromatography Columns & Media
`
`Carbohydrate Analysis by Ligand Exchange - PL Hi-Plex Columns
`
`Index
`
`33
`
`25
`
`35
`
`39
`
`48
`
`52
`
`56
`
`58
`
`60
`
`64
`
`65
`
`66
`
`68
`
`74
`
`75
`
`79
`
`83
`
`109
`
`115
`
`125
`
`133
`
`3
`
`

`

`Polymer Laboratories
`
`The Company
`
`Polymer Laboratories was founded in 1976 to develop techniques and instrumentation for the
`characterization of polymer systems, and to develop high technology polymer-based products
`for chromatographic, pharmaceutical and diagnostic applications.
`
`Batch to Batch Reproducibility Guaranteed
`Polymer Laboratories is dedicated to the highest
`standards of production, quality assurance and control.
`The production of PL media and resins is tightly
`controlled and monitored, ensuring product
`reproducibility from batch to batch throughout the entire
`particle/pore size ranges.
`
`Global Operation
`PL has offices in the USA, UK, The Netherlands,
`Germany and France, and for maximum global availability
`and service, PL products are also supported by a
`technical worldwide distributor network.
`
`The Success Factor
`The Company’s success is derived from its high level
`of skilled staff, including scientists, engineers and
`programmers specializing in polymer science, with
`product innovation being a keynote.
`
`Polymer Laboratories’ Product Assurance &
`Manufacturing Capabilities
`Polymer Laboratories is an ISO 9001:2000 Standard
`Quality Assessed Company to assure you of the quality
`and reliability of our products and service to you.
`
`Polymer Laboratories wins a SMART Award
`
`Manufacturing Facilities
`Polymer Laboratories has invested in high technology,
`large scale production facilities to ensure a reliable
`supply of quality media and resins to meet our
`customers’ growing needs. PL’s commitment to you, the
`User, is to provide rapid order turnaround times and
`quick delivery.
`
`Customer Support
`PL provides a high level of technical support for its
`product ranges, and offers a free applications service.
`A comprehensive range of technical support material is
`available, from reference papers to technical bulletins.
`Contact your local office or visit our website at
`www.polymerlabs.com.
`
`2
`
`www.polymerlabs.com
`
`4
`
`

`

`Organic GPC
`
`Columns for Organic GPC
`
`Organic GPC
`Columns for Organic GPC
`
`Gel Permeation Chromatography (GPC)
`is a high performance liquid chromatography technique for
`the separation of components based on their molecular
`size in solution. It is widely used as a means of characterizing
`the molecular weight distribution of polymers, and can also
`be employed for the separation of discrete components.
`
`Polymer Laboratories offers a comprehensive range of
`organic GPC columns for optimum performance and
`reliability, and long column lifetimes, even under the most
`severe operating conditions.
`
`NEW PlusPore - NEW High Resolution Columns
`for Organic GPC
`
`PolyPore
`ResiPore
`MesoPore
`OligoPore
`
`PLgel Advanced Organic GPC Columns
`
`PLgel MIXED Gel GPC Columns
`PLgel 20µm MIXED-A
`PLgel 10µm MIXED-B
`PLgel 5µm MIXED-C
`PLgel 5µm MIXED-D
`PLgel 3µm MIXED-E
`PLgel LS Columns for Light Scattering & Viscosity
`Narrow Bore GPC Columns
`PLgel Individual Pore Size GPC Columns
`PLgel Preparative GPC Columns
`
`PL EnviroPrep Columns
`
`PL HFIPgel Columns for GPC Applications in HFIP
`
`Organic GPC Column Ordering Information
`
`Organic GPC Column & Accessories
`Ordering Information
`
`4
`5
`6
`7
`8
`
`9
`10
`11
`12
`13
`14
`15
`16
`17
`18
`20
`
`21
`
`22
`
`23
`
`24
`
`753
`
`5
`
`

`

`Organic GPC
`
`PlusPore - NEW High Resolution Columns for Organic GPC
`
`PolyPore for the routine analysis of general polymers
`
`ResiPore for the analysis of resins and
`condensation polymers
`
`■ MesoPore for the analysis of prepolymers and
`low MW resins
`
`■ OligoPore for the analysis of oligomeric samples
`
`Features and Benefits of the PlusPore Range
`
`High pore volume, high resolution
`
`■ Wide pore size distribution
`
`Optimized separation range
`
`Full solvent compatibility
`
`No MWD dislocations
`
`PlusPore Calibration Curves
`6.5
`
`PolyPore
`ResiPore
`MesoPore
`OligoPore
`
`6
`
`5.5
`
`5
`
`4.5
`
`4
`
`3.5
`
`3
`
`2.5
`
`2
`
`Log MW
`
`4
`
`5
`
`7
`6
`Retention time / min
`
`8
`
`9
`
`10
`
`The PlusPore series of columns has been
`specifically designed for high resolution GPC, and
`represents the very latest in GPC column
`technology. These novel packing materials are
`based on the industry standard, highly crosslinked
`polystyrene/divinylbenzene (PS/DVB), for the
`widest applicability and solvent compatibility. Each
`is made using a novel polymerization process to
`produce particles which exhibit a specific,
`controlled pore structure for optimum GPC
`performance.
`
`The Ideal Choice for Polymer Analysis
`For high resolution polymer analysis, the PolyPore,
`ResiPore, MesoPore and OligoPore columns of the
`PlusPore product range exhibit a wide pore size
`distribution with near linear calibration curves covering an
`extended molecular weight range. These so-called
`‘multipore’ structures have increased pore volume
`compared to regular PS/DVB packing materials. This
`results in very high resolution GPC columns designed for
`specific application areas. The highly crosslinked porous
`particles provide excellent chemical and physical stability
`and permit easy transfer across the full range of organic
`solvents with little change in the shape of the calibration
`curve or the efficiency of the columns. As this ‘multipore’
`column technology does not require the combination of
`individual pore size packing materials, the result is high
`accuracy and precision without any artefacts in the shape
`of the molecular weight distribution.
`
`NEWPlusPore - High Resolution Columns
`Organic GPC
`
`PlusPore High Resolution Columns - Specifications
`PolyPore
`ResiPore
`MW Operating Range
`200-2,000,000
`200-400,000
`Nominal Particle Size
`5µm
`3µm
`Typical Column Efficiency >60,000 p/m
`>80,000 p/m
`Recommended Calibrants
`EasiCal PS-1 or EasiVial
`EasiCal PS-2
`
`MesoPore
`Up to 25,000
`3µm
`>80,000 p/m
`Polystyrene S-L-10 Kit
`Polyethylene Glycol
`PEG-10 Kit for DMF
`
`OligoPore
`Up to 4,500
`6µm
`>55,000 p/m
`Individual MW Polystyrenes
`
`4
`
`www.polymerlabs.com/gpc
`
`6
`
`■
`■
`■
`■
`■
`■
`

`

`PlusPore - NEW High Resolution Columns for Organic GPC
`
`PolyPore - for the Routine Analysis of General Polymers
`
`Organic GPC
`NEW... PolyPore Columns
`
`PolyPore
`ResiPore
`MesoPore
`OligoPore
`
`PlusPore Calibration Curves
`6.5
`
`6
`
`5.5
`
`5
`
`4.5
`
`4
`
`3.5
`
`3
`
`2.5
`
`2
`
`Log MW
`
`4
`
`5
`
`7
`6
`Retention time / min
`
`8
`
`9
`
`10
`
`Comparison of PolyPore with Conventional
`Individual Pore Size GPC Columns
`Sample:
`High MW Resin
`Columns:
`2xPolyPore, 300x7.5mm (1113-6500)
`PLgel 5µm 103Å, 300x7.5mm (1110-6530)
`PLgel 5µm 105Å, 300x7.5mm (1110-6550)
`THF
`1.0ml/min
`RI
`
`Eluent:
`Flow Rate:
`Detector:
`
`Individual Pore Size
`Columns
`
`PolyPore
`
`Typical Applications
`
`Polystyrenes
`
`Polycarbonates
`
`Polyurethanes
`
`Polysiloxanes
`
`PolyPore columns have been specifically
`developed to give unrivalled resolution for the
`analysis of polymers with broad molecular weight
`distributions. With a wide operating range covering
`many decades of molecular weight, PolyPore
`columns combine a low 5µm particle size with
`extremely high pore volume to give the highest
`possible resolution, ensuring you the most detailed
`information possible from your analysis.
`
`Polystyrene Standards
`Sample:
`EasiCal PS-1
`Columns:
`2xPolyPore, 300x7.5mm (1113-6500)
`Eluent:
`THF
`Flow Rate:
`1.0ml/min
`Detector:
`UV, 254nm
`
`5
`
`min
`
`20
`
`Polymethylmethacrylate in DMF
`Sample: Commercial PMMA
`Columns:
`2xPolyPore, 300x7.5mm (1113-6500)
`Eluent:
`0.1% LiBr in DMF
`Flow Rate:
`1.0ml/min
`Temp:
`80°C
`Detector:
`RI
`
`0
`
`min
`
`24
`
`5
`
`min
`
`20
`
`Specifications
`MW Operating Range
`Nominal Particle Size
`Typical Column Efficiency
`Recommended Calibrants
`
`200-2,000,000
`5µm
`>60,000 p/m
`EasiCal PS-1 or EasiVial
`
`Product
`PolyPore, 300x7.5mm
`PolyPore, 250x4.6mm
`PolyPore Guard, 50x7.5mm
`PolyPore Guard, 50x4.6mm
`
`Part No.
`1113-6500
`1513-5500
`1113-1500
`1513-1500
`
`US 800 767 3963
`
`To order please contact Polymer Laboratories, or your local distributor
`UK / International (+44) 01694 723581 Germany (+49) 06151 860690
`Benelux (+31) 045 5414748
`
`France (+33) 04 91 17 64 00
`
`755
`
`7
`
`☎
`■
`■
`■
`■
`

`

`PlusPore - NEW High Resolution Columns for Organic GPC
`
`ResiPore for the Analysis of Resins and
`Condensation Polymers
`
`PolyPore
`ResiPore
`MesoPore
`OligoPore
`
`PlusPore Calibration Curves
`6.5
`
`6
`
`5.5
`
`5
`
`4.5
`
`4
`
`3.5
`
`3
`
`2.5
`
`2
`
`Log MW
`
`Application Areas
`
`Epoxy resins
`
`Polyester resins
`
`Silicone fluids
`
`Polyolefin waxes
`
`ResiPore columns are the ideal choice for the
`analysis of resins and condensation polymers
`with complex molecular weight distributions
`including oligomer content. By combining a low
`3µm particle size and high pore volume, high
`efficiency ResiPore columns give the maximum
`resolution for the analysis of these intermediate
`molecular weight polymers.
`Epoxy Resin
`Columns:
`2xResiPore, 300x7.5mm (1113-6300)
`Eluent: THF
`Flow Rate: 1.0ml/min
`Inj Vol: 20µl
`Detector: RI
`
`NEW...ResiPore Columns
`Organic GPC
`
`4
`
`5
`
`7
`6
`Retention time / min
`
`8
`
`9
`
`10
`
`5
`
`min
`
`20
`
`Alkyd Resin
`Columns:
`2xResiPore, 300x7.5mm (1113-6300)
`Eluent: THF
`Flow Rate: 1.0ml/min
`Inj Vol: 20µl
`Detector: UV, 254nm
`
`Polyester
`Columns: 2xResiPore, 300x7.5mm (1113-6300)
`Eluent: THF
`Flow Rate: 1.0ml/min
`Inj Vol: 20µl
`Detector: UV, 254nm
`
`5
`
`min
`
`20
`
`5
`
`min
`
`20
`
`Specifications
`MW Operating Range
`Nominal Particle Size
`Typical Column Efficiency
`Recommended Calibrants
`
`200-400,000
`3µm
`>80,000 p/m
`EasiCal PS-2
`
`Product
`ResiPore, 300x7.5mm
`ResiPore, 250x4.6mm
`ResiPore Guard, 50x7.5mm
`ResiPore Guard, 50x4.6mm
`
`Part No.
`1113-6300
`1513-5300
`1113-1300
`1513-1300
`
`6
`
`www.polymerlabs.com/gpc
`
`8
`
`■
`■
`■
`■
`

`

`PlusPore - NEW High Resolution Columns for Organic GPC
`
`MesoPore for the Analysis of Prepolymers and Low MW Resins
`Typical Applications
`
`Organic GPC
`NEW MesoPore Columns
`
`PolyPore
`ResiPore
`MesoPore
`OligoPore
`
`PlusPore Calibration Curves
`6.5
`
`6
`
`5.5
`
`5
`
`4.5
`
`4
`
`3.5
`
`3
`
`2.5
`
`2
`
`Log MW
`
`4
`
`5
`
`7
`6
`Retention time / min
`
`8
`
`9
`
`10
`
`Polyesterimide
`Columns:
`2xMesoPore, 300x7.5mm (1113-6325)
`Eluent: THF
`Flow Rate: 1.0ml/min
`Inj Vol: 20µl
`Detector: RI
`
`I Prepolymers
`
`I Resins
`
`I Polyols
`
`I Siloxanes
`
`MesoPore columns have been specifically
`designed to give optimum results for the
`analysis of prepolymers: polymeric materials
`with a large oligomeric component. By combining
`a 3µm particle size with high pore volume,
`MesoPore columns give the highest resolution
`separations for the analysis of low molecular
`weight polymers.
`
`Epoxy Resin
`Columns:
`2xMesoPore, 300x7.5mm (1113-6325)
`Eluent: THF
`Flow Rate: 1.0ml/min
`Inj Vol: 20µl
`Detector: RI
`
`5
`
`min
`
`20
`
`Polyurethanes
`Columns:
`2xMesoPore, 300x7.5mm (1113-6325)
`Eluent: THF
`Flow Rate: 1.0ml/min
`Inj Vol: 20µl
`Detector: RI
`
`5
`
`min
`
`22
`
`5
`
`min
`
`17
`
`Specifications
`MW Operating Range
`Nominal Particle Size
`Typical Column Efficiency
`Recommended Calibrants
`
`Up to 25,000
`3µm
`>80,000 p/m
`Polystyrene S-L-10 Kit
`Polyethylene Glycol PEG-10
`Kit for DMF
`
`Product
`MesoPore, 300x7.5mm
`MesoPore, 250x4.6mm
`MesoPore Guard, 50x7.5mm
`MesoPore Guard, 50x4.6mm
`
`Part No.
`1113-6325
`1513-5325
`1113-1325
`1513-1325
`
` US 800 767 3963
`
`To order please contact Polymer Laboratories, or your local distributor
`UK / International (+44) 01694 723581 Germany (+49) 06151 860690
`Benelux (+31) 045 5414748
`
`France (+33) 04 91 17 64 00
`
`757
`
`9
`
`

`

`PlusPore - NEW High Resolution Columns for Organic GPC
`
`OligoPore for the Analysis of Oligomeric Samples
`Typical Applications
`OligoPore Preparative Column
`The 300x25mm preparative column offers high resolution
`at greatly increased loading for effective isolation of
`individual components.
`
`Polyurethanes
`
`Epoxy resins
`
`Polystyrenes
`
`Analytical Separation
`Columns:
`2xOligoPore, 300x7.5mm (1113-6520)
`Eluent:
`THF
`Flow Rate:
`1.0ml/min
`Loading:
`0.2%, 100ml
`Detector:
`UV
`
`5
`
`min
`
`20
`
`Preparative Separation
`Columns:
`2xOligoPore, 300x25mm (1513-6520)
`Eluent:
`THF
`Flow Rate:
`10.0ml/min
`Loading:
`2.0%, 2ml
`Detector:
`UV
`
`OligoPore columns have been developed from an
`innovative new media, which exhibits significantly
`increased pore volumes compared to conventional
`low pore size GPC columns, resulting in higher
`resolution in the oligomeric region.
`
`PolyPore
`ResiPore
`MesoPore
`OligoPore
`
`PlusPore Calibration Curves
`6.5
`
`6
`
`5.5
`
`5
`
`4.5
`
`4
`
`3.5
`
`3
`
`2.5
`
`2
`
`Log MW
`
`OligoPore Columns
`Organic GPC
`
`4
`
`5
`
`7
`6
`Retention time / min
`
`8
`
`9
`
`10
`
`Polystyrene Standards
`Columns:
`2xOligoPore, 300x7.5mm (1113-6520)
`Eluent:
`THF
`Flow Rate:
`1.0ml/min
`Detector:
`RI
`
`1,270
`
`5
`
`min
`
`20
`
`Oligomer fractions collected from the OligoPore
`preparative column can then be re-injected on analytical
`columns to check for the purity of the fractions and for
`comparison with the whole sample.
`
`580
`
`Analysis of Whole Polymer and Fractions
`Collected from OligoPore Preparative Columns
`
`5
`
`min
`
`20
`
`Epoxy Resin (Epikote 1001)
`Columns:
`2xOligoPore, 300x7.5mm (1113-6520)
`Eluent:
`THF
`Flow Rate:
`1.0ml/min
`Detector:
`RI
`
`Polystyrene Oligomers
`Columns:
`2xOligoPore, 300x7.5mm (1113-6520)
`Eluent:
`THF
`Flow Rate:
`1.0ml/min
`Detector:
`UV
`
`Whole polymer
`F9
`F8
`F7
`F6
`F5
`F4
`F3
`F2
`F1
`
`5
`
`min
`
`20
`
`10
`
`min
`
`20
`
`Specifications
`MW Operating Range
`Nominal Particle Size
`Typical Column Efficiency
`Recommended Calibrants
`
`8
`
`Up to 4,500
`6µm
`>55,000 p/m
`Individual MW
`Polystyrenes
`
`Product
`OligoPore, 300x7.5mm
`OligoPore, 250x4.6mm
`OligoPore, 300x25mm
`OligoPore Guard, 50x7.5mm
`OligoPore Guard, 50x4.6mm
`
`Part No.
`1113-6520
`1513-5520
`1213-6520
`1113-1320
`1513-1320
`
`www.polymerlabs.com/gpc
`
`10
`
`■
`■
`■
`

`

`Organic GPC
`
`PLgel Advanced Organic GPC Columns
`Solvent Compatibility
`PLgel is a highly crosslinked porous polystyrene/
`PLgel columns are routinely supplied in toluene*,
`divinylbenzene matrix which is recognized as a
`however, they can be transferred easily and rapidly
`market leader in GPC column technology.
`between solvents of varying polarity by the User. In
`organic GPC, sample to column interaction can occur
`occasionally and eluent modification can be used to
`eliminate the effects. PLgel columns are the ideal choice
`for these analyses, as they easily tolerate eluents in the
`pH range 1-14, as well as up to 10% water, in a miscible
`organic solvent.
`
`Manufactured and packed exclusively by Polymer
`Laboratories since 1976, PLgel has very special features:
`
`High pore volume and high efficiency for maximum
`resolution
`
`Organic GPC
`PLgel Columns
`
`*PL also provides a custom packing service in which columns can be
`shipped in specific solvents to provide extra convenience to
`our customers.
`
`Unequalled solvent compatibility for transfers
`between polar and non-polar organic eluents
`
`Outstanding physical rigidity for extended lifetimes
`especially at high temperatures and in aggressive
`solvents
`
`PL’s comprehensive QC/QA for total reproducibility,
`batch to batch, column to column
`
`Low
`
`■ Manufactured to ISO 9001
`
`The key to successful GPC
`separations is the correct
`choice of columns. The
`comprehensive range of
`PLgel products has been
`designed to cover virtually
`all organic solvent based
`polymer analysis application
`areas, and to make
`selection of the correct
`column, solvent and
`calibration standards fast
`and reliable.
`
`High
`
`Solvent
`Polarity
`6.0
`7.3
`8.2
`8.9
`9.1
`9.1
`9.3
`9.3
`9.7
`9.8
`9.9
`10.0
`10.0
`10.2
`10.2
`10.7
`10.8
`11.3
`12.0
`12.1
`
`Solvent
`
`PLgel
`Compatibility
`
`Perfluoralkanes
`Hexane
`Cyclohexane
`Toluene
`Ethyl acetate
`Tetrahydrofuran (THF)
`Chloroform
`Methyl ethyl ketone (MEK)
`Dichloromethane
`Dichloroethane
`Acetone
`o-Dichlorobenzene (o-DCB)
`Trichlorobenzene (TCB)
`m-Cresol
`o-Chlorophenol (o-CP)
`Pyridine
`Dimethyl acetamide (DMAc)
`n-Methyl pyrolidone (NMP)
`Dimethyl sulphoxide (DMSO)
`Dimethyl formamide (DMF))
`
`The PLgel Column Range Includes:
`Seven individual pore size packings, 50Å to 106Å
`
`Five linear MIXED gel packings, A to E
`
`Four analytical particle sizes, 3µm, 5µm, 10µm, 20µm
`
`Column lengths 300mm and 600mm
`
`Temperature Stability to 220°C
`Elevated temperature is used in GPC either to reduce
`eluent viscosity (eg polar solvent applications), or to
`maintain sample solubility (eg polyolefin applications).
`PLgel columns can be used at temperatures up to 220°C
`and operating pressures up to 150 bar (2200psi).
`
`4.6mm ID narrow bore columns
`
`7.5mm ID analytical columns
`
`25mm ID preparative columns
`
`50mm guard columns
`
`US 800 767 3963
`
`To order please contact Polymer Laboratories, or your local distributor
`UK / International (+44) 01694 723581 Germany (+49) 06151 860690
`Benelux (+31) 045 5414748
`
`France (+33) 04 91 17 64 00
`
`759
`
`11
`
`☎
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`■
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`■
`■
`■
`■
`■
`■
`■
`■
`■
`✓
`✓
`✓
`✓
`✓
`✓
`✓
`✓
`✓
`✓
`✓
`✓
`✓
`✓
`✓
`✓
`✓
`✓
`✓
`✓
`

`

`Organic GPC
`
`PLgel MIXED Gel GPC Columns
`PLgel MIXED Gel Calibration Curves
`A significant number of GPC applications involve
`the analysis of polydisperse materials. The modern
`approach to column selection is to choose
`PL’s MIXED gel columns, where each column
`contains a mixture of
`individual pore size
`materials, accurately blended to cover a specified
`broad range of molecular weight with a linear
`calibration to eliminate column mismatch. As
`market leaders in this field, Polymer Laboratories
`offers a comprehensive range of MIXED gel GPC
`columns designed for specific application areas.
`
`10M
`
`1M
`
`100K
`
`10K
`
`1K
`
`Polystyrene Molecular Weight
`
`KEY
`20µm MIXED-A
`10µm MIXED-B
`5µm MIXED-C
`5µm MIXED-D
`3µm MIXED-E
`
`PLgel MIXED Gel GPC Columns
`Organic GPC
`
`Key Advantages of PLgel MIXED Columns
`Include:
`Greatly simplified column selection
`
`Improved confidence in the accuracy and precision
`of calculated data
`
`Optimized particle size for each application area
`
`Reduced replacement stock
`
`Elimination of mismatched column sets and
`spurious peaks
`
`Simple addition of extra column(s) for greater
`resolution
`
`4
`
`6
`5
`7
`8
`Elution Volume (ml)
`
`9
`
`10
`
`The calibration curves are designed to be linear over a
`specified molecular weight range, ensuring that the same
`degree of resolution is achieved across the full operating
`range of the column.
`
`The particle size of the packing and the porosity of a
`particular MIXED gel column are carefully matched to the
`MW range and application, thus optimizing performance
`and eliminating the effects of shear degradation.
`
`Resolution in GPC is controlled by the slope of the
`calibration curve and the particle size of the packing
`material. PL has scientifically determined the minimum
`number of MIXED gel columns required to perform
`accurate MWD determinations based on specific
`resolution (Rsp).
`
`Ref: “Size exclusion chromatography columns from Polymer
`Laboratories”, in Column Handbook for Size Exclusion Chromatography,
`ed. Chi-san Wu, Academic Press, 1999.
`
`PLgel MIXED Gel Column Selection Guide
`
`UHMW polymer
`distributions
`
`High MW polymers,
`demanding eluents
`
`PLgel 20µm MIXED-A
`
`PLgel 10µm MIXED-B
`
`Mid range MW polymers,
`high resolution
`
`PLgel 5µm MIXED-C
`
`Resins, condensation
`polymers
`
`Low MW resins,
`prepolymers
`
`PLgel 5µm MIXED-D
`
`PLgel 3µm MIXED-E
`
`10
`
`102
`
`103
`
`104
`
`105
`
`106
`
`107
`
`www.polymerlabs.com/gpc
`
`12
`
`■
`■
`■
`■
`■
`■
`●●
`●
`■
`▲
`■■
`

`

`Organic GPC
`PLgel 20µm MIXED-A Columns
`
`Organic GPC
`
`PLgel 20µm MIXED-A Columns
`Typical Applications
`Polymers containing significant quantities of ultra high
`MW material which are relatively polydisperse
`
`Polyolefins
`
`Starches
`
`Polybutadienes
`
`Polyisoprenes
`
`Polystyrene Standards Separation
`Sample:
`EasiCal PS-1
`Columns:
`4xPLgel 20µm MIXED-A, 300x7.5mm (1110-6200)
`Eluent:
`THF
`Flow Rate:
`1.0ml/min
`Detector:
`UV, 254nm
`
`3
`
`4
`
`5
`
`2
`
`1
`
`6
`
`8
`
`9
`
`7
`
`10
`
`EasiCal PS-1
`1. 7,500,000
`2. 841,700
`3. 148,000
`4. 28,500
`5. 2,930
`6. 2,560,000
`7. 320,000
`8. 59,500
`9. 10,850
`10. 580
`
`Starches
`Columns:
`Eluent:
`Flow Rate:
`Temp:
`Detector:
`
`10
`
`min
`
`40
`
`4xPLgel 20µm MIXED-A, 300x7.5mm (1110-6200)
`DMSO + 5mM NaNO3
`1.0ml/min
`80°C
`RI
`
`5
`
`min
`
`44
`
`Polyolefins
`Columns:
`4xPLgel 20µm MIXED-A, 300x7.5mm (1110-6200)
`Eluent:
`TCB
`Flow Rate:
`1.0ml/min
`Temp:
`160°C
`Detector:
`RI
`
`5
`
`min
`
`44
`
`Polyethylene on Narrow Bore MiniMIX Columns
`Columns:
`4xPLgel 20µm MiniMIX-A, 250x4.6mm (1510-5200)
`Eluent:
`TCB
`Flow Rate:
`0.3ml/min
`Loading:
`0.1% w/v, 100µl
`Temp:
`140°C
`Detector:
`IR
`
`Features
`Extremely high
`exclusion limit
`(40,000,000)
`
`■ Matched frit porosity
`
`Low shear
`
`Large particle size
`
`Linear calibration
`
`PLgel MIXED Gel Calibration Curves
`
`10M
`
`1M
`
`100K
`
`10K
`
`1K
`
`Polystyrene Molecular Weight
`
`Key
`20µm MIXED-A
`10µm MIXED-B
`5µm MIXED-C
`5µm MIXED-D
`3µm MIXED-E
`
`4
`
`6
`5
`7
`8
`Elution Volume (ml)
`
`9
`
`10
`
`Maximum flow rate @ 7.5mm ID:
`@ 4.6mm ID:
`
`PLgel 20µm MIXED-A Specifications
`Linear range of MW:
`2,000 - 40,000,000
`Guaranteed column efficiency:
`>17,000 plates/m
`Typical pressure @ 1.0ml/min (7.5mm ID):
`≈3 bar (45psi) per 300mm
`@ 0.3ml/min (4.6mm ID):
`≈2.4 bar (35psi) per 250mm
`(THF @ 20°C) (TCB @ 140°C)
`1.5ml/min
`0.5ml/min
`150 bar
`Maximum pressure:
`220°C
`Maximum temperature:
`Recommended no. of columns in set: 4 x 250mm, 4 x 300mm
`or 2 x 600mm
`Part No.
`Product
`1110-6200
`PLgel 20µm MIXED-A, 300x7.5mm
`1510-5200
`PLgel 20µm MiniMIX-A, 250x4.6mm
`1110-8200
`PLgel 20µm MIXED-A, 600x7.5mm
`1110-1220
`PLgel 20µm Guard, 50x7.5mm
`PLgel 20µm MiniMIX-A Guard, 50x4.6mm 1510-1200
`
`Polystyrene Calibration
`EasiVial PS - convenient 12 point calibration in just 3 injections
`EasiCal® PS-1 or S-H2-10 Kit - rapid 10 point calibration
`S-H-10 plus S-M2-10 Kits - accurate 19 point calibration
`
`Product
`EasiVial PS
`EasiCal® PS-1
`Polystyrene Calibration Kit S-H-10
`Polystyrene Calibration Kit S-H2-10
`Polystyrene Calibration Kit S-M2-10
`
`Part No.
`2010-0200
`2010-0501
`2010-0103
`2010-0104
`2010-0102
`
`US 800 767 3963
`
`To order please contact Polymer Laboratories, or your local distributor
`UK / International (+44) 01694 723581 Germany (+49) 06151 860690
`Benelux (+31) 045 5414748
`
`France (+33) 04 91 17 64 00
`
`7511
`
`20
`
`min
`
`40
`
`13
`
`■
`■
`■
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`■
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`●●
`●
`■
`▲
`■■
`

`

`Organic GPC
`
`Features
`■ Wide operating
`range of MW
`(500-10,000,000)
`
`Linear calibration
`
`Low operating
`pressure
`
`■ Wide range of
`applications
`
`PLgel 10µm MIXED-B Columns
`Typical Applications
`General polymer analysis covering a wide range of MW,
`especially applications which use demanding conditions,
`eg high temperature, aggressive or unusual solvents
`
`Polyolefins
`
`Fluoropolymers
`
`Acrylics/Acrylates
`
`Cellulose derivatives
`
`PLgel MIXED Gel Calibration Curves
`
`Polystyrene Standards Separation
`Sample:
`EasiCal PS-1
`Columns:
`3xPLgel 10µm MIXED-B, 300x7.5mm (1110-6100)
`Eluent:
`THF
`Flow Rate:
`1.0ml/min
`Detector:
`UV, 254nm
`
`3
`
`4
`
`5
`
`2
`
`1
`
`EasiCal PS-1
`1. 7,500,000
`2. 841,700
`3. 148,000
`4. 28,500
`5. 2,930
`6. 2,560,000
`7. 320,000
`8. 59,500
`9. 10,850
`10. 580
`
`8
`
`7
`
`9
`
`6
`
`10
`
`5
`
`min
`
`30
`
`Polyphenylene Sulfides
`Columns:
`3xPLgel 10µm MIXED-B, 300x7.5mm (1110-6100)
`Eluent:
`o-Chloronaphthalene
`Flow Rate:
`1.0ml/min
`Temp:
`210°C
`Detector:
`RI
`
`10
`
`min
`
`30
`
`Polyethylenes
`Columns:
`3xPLgel 10µm MIXED-B, 300x7.5mm (1110-6100)
`Eluent:
`TCB
`Flow Rate:
`1.0ml/min
`Temp:
`160°C
`Detector:
`RI
`
`HDPE
`
`LDPE
`
`5
`
`min
`
`30
`
`Polystyrene on Narrow Bore MiniMIX Columns
`Columns:
`3xPLgel 10µm MiniMIX-B, 250x4.6mm (1510-5100)
`Eluent:
`THF
`Flow Rate:
`0.3ml/min
`Loading:
`0.2% w/v, 20µl
`Detector:
`UV, 254nm
`
`14
`
`min
`
`32
`
`10M
`
`1M
`
`100K
`
`Polystyrene Molecular Weight
`
`10K
`
`1K
`
`4
`
`6
`5
`7
`8
`9
`Elution Volume (ml)
`
`10
`
`Key
`20µm MIXED-A
`10µm MIXED-B
`5µm MIXED-C
`5µm MIXED-D
`3µm MIXED-E
`
`Maximum flow rate @ 7.5mm ID:
`@ 4.6mm ID:
`
`PLgel 10µm MIXED-B Specifications
`Linear range of MW:
`500 - 10,000,000
`Guaranteed column efficiency:
`>35,000 plates/m
`Typical pressure @ 1.0ml/min (7.5mm ID):
`≈10 bar (150psi) per 300mm
`@ 0.3ml/min (4.6mm ID):
`≈8 bar (120psi) per 250mm
`(THF @ 20°C) (TCB @ 140°C)
`1.5ml/min
`0.5ml/min
`150 bar
`Maximum pressure:
`220°C
`Maximum temperature:
`Recommended no. of columns in set: 3 x 250mm, 3 x 300mm
`or 1-2 x 600mm
`Part No.
`Product
`1110-6100
`PLgel 10µm MIXED-B, 300x7.5mm
`1510-5100
`PLgel 10µm MiniMIX-B, 250x4.6mm
`1110-8100
`PLgel 10µm MIXED-B, 600x7.5mm
`1110-1120
`PLgel 10µm Guard, 50x7.5mm
`PLgel 10µm MiniMIX-B Guard, 50x4.6mm 1510-1100
`
`Polystyrene Calibration
`EasiVial PS - convenient 12 point calibration in just 3 injections
`EasiCal® PS-1 or S-H2-10 Kit - rapid 10 point calibration
`S-H-10 plus S-M2-10 Kits - accurate 19 point calibration
`
`Product
`EasiVial PS
`EasiCal® PS-1
`Polystyrene Calibration Kit S-H-10
`Polystyrene Calibration Kit S-H2-10
`Polystyrene Calibration Kit S-M-10
`Polystyrene Calibration Kit S-M2-10
`
`Part No.
`2010-0200
`2010-0501
`2010-0103
`2010-0104
`2010-0100
`2010-0102
`
`PLgel 10µm MIXED-B Columns
`Organic GPC
`
`12
`
`www.polymerlabs.com/gpc
`
`14
`
`■
`■
`■
`■
`■
`■
`●●
`●
`■
`▲
`■■
`

`

`Organic GPC
`PLgel 5µm MIXED-C Columns
`
`Organic GPC
`
`PLgel 5µm MIXED-C Columns
`Typical Applications
`For polymer analysis up to 2 million MW, especially
`polymers containing small molecule additives
`
`Polystyrenes
`
`Polyurethanes
`
`Polycarbonates
`
`Polysiloxanes
`
`3
`
`4
`
`Polystyrene Standards Separation
`Sample:
`EasiCal PS-1
`Columns:
`2xPLgel 5µm MIXED-C, 300x7.5mm (1110-6500)
`Eluent:
`THF
`Flow Rate:
`1.0ml/min
`Detector:
`UV, 254nm
`
`1 2
`
`5
`
`EasiCal PS-1
`1. 7,500,000
`2. 841,700
`3. 148,000
`4. 28,500
`5. 2,930
`6. 2,560,000
`7. 320,000
`8. 59,500
`9. 10,850
`10. 580
`
`8
`
`7
`
`9
`
`6
`
`10
`
`5
`
`min
`
`20
`
`Plasticized PVC
`Columns:
`3xPLgel 5µm MIXED-C, 300x7.5mm (1110-6500)
`Eluent:
`THF
`Flow Rate:
`1.0ml/min
`Detector:
`RI
`
`5
`
`min
`
`33
`
`Polyurethane
`Columns:
`2xPLgel 5µm MIXED-C, 300x7.5mm (1110-6500)
`Eluent:
`DMF + 0.1% LiBr
`Flow Rate:
`1.0ml/min
`Temp:
`80°C
`Detector:
`RI
`
`3
`
`min
`
`22
`
`Polymethylmethacrylate on Narrow Bore
`MiniMIX Columns
`Columns:
`2xPLgel 5µm MiniMIX-C,
`250x4.6mm (1510-5500)
`DMF
`0.3ml/min
`0.1% w/v, 20µl
`RI
`
`Eluent:
`Flow Rate:
`Loading:
`Detector:
`
`6
`
`min
`
`17
`
`Features
`High efficiency
`
`Linear calibration
`
`Excellent solvent
`compatibility
`
`Fast analysis
`
`PLgel MIXED Gel Calibration Curves
`
`10M
`
`1M
`
`100K
`
`10K
`
`1K
`
`Polystyrene Molecular Weight
`
`Key
`20µm MIXED-A
`10µm MIXED-B
`5µm MIXED-C
`5µm MIXED-D
`3µm MIXED-E
`
`4
`
`6
`5
`7
`8
`Elution Volume (ml)
`
`9
`
`10
`
`Maximum flow rate @ 7.5mm ID:
`@ 4.6mm ID:
`
`PLgel 5µm MIXED-C Specifications
`Linear range of MW:
`200 - 2,000,000
`Guaranteed column efficiency:
`>50,000 plates/m
`Typical pressure @ 1.0ml/min (7.5mm ID):
`≈30 bar (450psi) per 300mm
`@ 0.3ml/min (4.6mm ID):
`≈24 bar (350psi) per 250mm
`(THF @ 20°C) (TCB @ 140°C)
`1.5ml/min
`0.5ml/min
`150 bar
`Maximum pressure:
`150°C
`Maximum temperature:
`Recommended no. of columns in set: 2 x 250mm, 2 x 300mm
`or 1 x 600mm
`Part No.
`Product
`1110-6500
`PLgel 5µm MIXED-C, 300x7.5mm
`1510-5500
`PLgel 5µm MiniMIX-C, 250x4.6mm
`1110-8500
`PLgel 5µm MIXED-C, 600x7.5mm
`1110-1520
`PLgel 5µm Guard, 50x7.5mm
`PLgel 5µm MiniMIX-C Guard, 50x4.6mm 1510-1500
`
`Calibration
`Polystyrene EasiVial PS
`Polystyrene EasiCal® PS-1
`Polystyrene S-M-10 Kit
`Polyethylene Oxide/Glycol PEO-10 / PEG-10 Kits for DMF
`
`Product
`EasiVial PS
`EasiCal® PS-1
`Polystyrene Calibration Kit S-M-10
`Polyethylene Oxide Kit PEO-10
`Polyethylene Glycol Kit PEG-10
`
`Part No.
`2010-0200
`2010-0501
`2010-0100
`2080-0101
`2070-0100
`
`US 800 767 3963
`
`To order please contact Polymer Laboratories, or your local distributor
`UK / International (+44) 01694 723581 Germany (+49) 06151 860690
`Benelux (+31) 045 5414748
`
`France (+33) 04 91 17 64 00
`
`7513
`
`15
`
`■
`■
`■
`■
`■
`■
`■
`■
`☎
`●●
`●
`■
`▲
`■■
`

`

`Organic GPC
`
`Features
`High efficiency
`
`High pore volume
`
`PLgel 5µm MIXED-D Columns
`Typical Applications
`Ideal for condensation polymers, paints and resins which
`may contain some low molecular weight oligomeric species
`
`Epoxy resins
`
`Silicone fluids
`
`Polyester resins
`
`Polyolefins
`
`Optimized linear
`resolving range
`
`Polymer plus some
`oligomer resolution
`
`Polystyrene Standards Separation
`Sample:
`EasiCal PS-2
`Columns:
`2xPLgel 5µm MIXED-D, 300x7.5mm (1110-6504)
`Eluent:
`THF
`Flow Rate
`1.0ml/min
`Detector:
`UV, 254nm
`
`3
`
`4
`
`2
`
`11
`
`5
`
`8
`
`7
`
`6
`
`9
`
`10
`
`EasiCal PS-2
`1. 380,000
`2. 96,000
`3. 22,000
`4. 5,050
`5. 1,320
`6. 156,000
`7. 49,900
`8. 11,600
`9. 2,950
`10. 580
`
`5
`
`min
`
`18
`
`Epoxy Resin
`Columns:
`3xPLgel 5µm MIXED-D,
`300x7.5mm (1110-6504)
`THF
`1.0ml/min
`RI
`
`Eluent:
`Flow Rate:
`Detector:
`
`5
`
`min
`
`33
`
`Asphalts
`Columns:
`Eluent:
`Flow Rate:
`Detector:
`
`2xPLgel 5µm MIXED-D, 300x7.5mm (1110-6504)
`THF
`1.0ml/min
`RI
`
`3
`
`min
`
`22
`
`Epoxy Resin on Narrow Bore MiniMIX Columns
`Columns:
`2xPLgel 5µm MiniMIX-D, 250x4.6mm (1510-5504)
`Eluent:
`THF
`Flow Rate:
`0.3ml/min
`Loading:
`0.1% w/v, 20µl
`Detector:
`UV, 254nm
`
`10
`
`min
`
`22
`
`PLgel MIXED Gel Calibration Curves
`
`10M
`
`1M
`
`100K
`
`10K
`
`1K
`
`Polystyrene Molecular Weight
`
`Key
`20µm MIXED-A
`10µm MIXED-B
`5µm MIXED-C
`5µm MIXED-D
`3µm MIXED-E
`
`4
`
`6
`5
`7
`8
`Elution Volume (ml)
`
`9
`
`10
`
`Maximum flow rate @ 7.5mm ID:
`@ 4.6mm ID:
`
`PLgel 5µm MIXED-D Specifications
`Linear range of MW:
`200 - 400,000
`Guaranteed column efficiency:
`>50,000 plates/m
`Typical pressure @ 1.0ml/min (7.5mm ID):
`≈50 bar (450psi) per 300mm
`@ 0.3ml/min (4.6mm ID):
`≈24 bar (350psi) per 250mm
`(THF @ 20°C) (TCB @ 140°C)
`1.5ml/min
`0.5ml/min
`150 bar
`Maximum pressure:
`150°C
`Maximum temperature:
`Recommended no. of columns in set: 2 x 250mm, 2 x 300mm
`or 1 x 600mm
`Part No.
`Product
`1110-6504
`PLgel 5µm MIXED-D, 300x7.5mm
`1510-5504
`PLgel 5µm MiniMIX-D, 250x4.6mm
`1110-8504
`PLgel 5µm MIXED-D, 600x7.5mm
`1110-1520
`PLgel 5µm Guard, 50x7.5mm
`PLgel 5µm MiniMIX-D Guard, 50x4.6mm 1510-1504
`
`Calibration
`EasiVial PS
`EasiCal® PS-2
`Polystyrene S-M2-10 Kit
`Polyethylene Oxide/Glycol PEO-10 / PEG-10 Kits for DMF
`
`Product
`EasiVial PS
`EasiCal® PS-2
`Polystyrene Calibration Kit S-M2-10
`Polyethylene Oxide Kit PEO-10
`Polyethylene Glycol Kit PEG-10
`
`Part No.
`2010-0200
`2010-0601
`2010-0102
`2080-0101
`2070-0100
`
`PLgel 5µm MIXED-D Columns
`Organic GPC
`
`14
`
`www.polymerlabs.com/gpc
`