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`Termination of DNA synthesis by novel 3'-
`modifieddeoxyribonucleoside 5'-triphosphates
`Michael L. Metzker , Ramesh Raghavachari, Stephen Richards, Swanee E. Jacutin,
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`Andrew Civitello, Kevin Burgess, Richard A. Gibbs
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`Nucleic Acids Research, Volume 22, Issue 20, 11 October 1994, Pages 4259–4267,
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`https://doi.org/10.1093/nar/22.20.4259
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`Published: 11 October 1994
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`Abstract
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`Eight 3′-modified-dNTPs were synthesized and tested in two different DNA template
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`assays for incorporation activity. From this enzymatic screen, two 3′-O-methyldNTPs
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`were shown to terminate DNA syntheses mediated by a number of polymerases and
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`may be used as alternative terminators in Sanger sequencing. 3-O-(2-Nitrobenzyl)
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`-dATP is a UV sensitive nucleotide and was shown to be incorporated by several
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`thermostable DNA polymerases. Base specific termination and efficient photolytic
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`removal of the 3′-protecting group was demonstrated. Following deprotection, DNA
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`synthesis was reinitiated by the incorporation of natural nucleotides into DNA. The
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`identification of this labile terminator and the demonstration of a one cycle stopstart
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`DNA synthesis are initial steps in the development of a novel sequencing strategy.
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`Issue Section: CHEMISTRY
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`Illumina Ex. 1097
`Illumina v. Columbia
`IPR2018-00291, -00318, -00322,
`-00385, and -00797
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