ANNE SKAJA ROBINSON
`anne.robinson@cmu.edu
`(412) 268-7673 PH
`
`
`EDUCATION
`1994 Ph.D., Chemical Engineering
`1989 M.S., Chemical Engineering
`1988 B.S., Chemical Engineering
`
`University of Illinois at Urbana-Champaign
`The Johns Hopkins University
`The Johns Hopkins University
`
`
`PROFESSIONAL EXPERIENCE
`
`
`
`Carnegie Mellon University, College of Engineering
`2018-present
`Head, Chemical Engineering
`
`
`Tulane University, School of Science and Engineering, Chemical and Biomolecular Engineering
`2018-present
`Adjunct Professor, Dept of Chemical and Biomolecular Engineering
`
`Chair, Chemical and Biomolecular Engineering and
`2012-2018
`Catherine and Henry Boh Professor in Engineering
`
`
`
`University of Delaware, College of Engineering
`Adjunct Professor, Department of Chemical and Biomolecular Engineering
`2012-present
`
`Professor and Associate Chair for Biochemical Engineering
`2008-2011
`
`Associate Professor
`2003-2008
`
`Assistant Professor
`1997-2003
`
`Massachusetts Institute of Technology, Department of Biology
`1994-1997
`Postdoctoral Fellow
`
`2015
`2013
`
`2012
`2011
`2002
`2000-2005
`
`Awards and Honors
`2016
`Fellow, American Institute of Chemical Engineers
`2015
`Biotechnology Progress Award for Excellence in Biological Engineering Publication, Society for
`Biological Engineering (SBE), an AIChE Technological Community
`Perlman Awardee, American Chemical Society (ACS) BIOT division
`American Institute of Chemical Engineers (AIChE) Women's Initiatives Committee's
`(WIC) Mentorship Excellence Award
`Van Lanen Award, American Chemical Society BIOT Division
`Fellow, American Institute for Medical and Biological Engineering
`Outstanding Junior Faculty Member, College of Engineering, University of Delaware
`NSF Presidential Early Career Award in Science and Engineering (PECASE/Career)
`PECASE award for subset of Career Award winners – 20 awarded for all NSF Directorates
`in 2000, of which only 2 were to chemical engineers.
`DuPont Young Professor
`National Academy of Engineering, Sixth Annual Frontiers in Engineering,
`One of 100 invited participants, Irvine, CA
`NIH Postdoctoral Fellowship
`Clare Booth Luce Graduate Fellowship
`Department of Defense Fellowship (NDSEG)
`NSF Creativity Award for Scientists and Engineers
`~20 awarded/yr following review of proposal and personal interview
`National Science Foundation Fellowship awarded
`
`
`2000-2003
`2000
`
`1996-97
`1992-94
`1989-92
`1988-89
`
`1988
`
`
`
`Page 1
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`KASHIV EXHIBIT 1003
`IPR2019-00791
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`Robinson, A.S.
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`PATENTS
`A) Kelly, R.M., A.K.S. Robinson, I.I. Blumentals, S.H. Brown, and C.B. Anfinsen. “Proteolytic
`Enzymes from Hyperthermophilic Bacteria and Processes for Their Production.” Patent #
`5,242,817. Filed 9/12/89. Accepted 9/7/93. Licensed to Takara Shuzo.
`B) Robinson, A.S. and K.D. Wittrup. “Methods for Increasing Secretion of Overexpressed Proteins.”
`Patent # 5,773,245. Filed 10/92. Accepted 6/30/98.
`C) Robinson, A.S., D. Foguel, J.L. Silva, C.R. Robinson. “Use of Hydrostatic Pressure to Inhibit and
`Reverse Protein Aggregation and Facilitate Protein Refolding.” US Patent # 7,615,617. Filed
`10/99. Issued 11/10/09.
`
`BOOK PUBLICATIONS
`
`
`1)
`
`Russell, T.W.F., Robinson, A.S., and Wagner, N.J., (2008) Mass and Heat Transfer: Analysis of Mass
`Contactors and Heat Exchangers, Cambridge University Press, Cambridge, UK
`(www.cambridge.org/9780521886703).
`Robinson, A.S., ed (2011) Production of Membrane Proteins – Strategies for Expression and Isolation,
`Wiley VCH (http://onlinelibrary.wiley.com/book/10.1002/9783527634521)
`
`JOURNAL PUBLICATIONS (* = CORRESPONDING AUTHOR, † = UNDERGRADUATE STUDENT)
`1) Blumentals, I. I., R. M. Kelly, A. K. Skaja [Robinson] and J. Shiloach* (1987) "Effect of Culturing
`Conditions on the Production of Exotoxin A by Pseudomonas aeruginosa." Ann N Y Acad. Sci.
`506, 663-668.
`2) Blumentals, I. I., A. S. Robinson and R. M. Kelly* (1990) "Characterization of Sodium Dodecyl
`Sulfate Resistant Proteolytic Activity in the Hyperthermophilic Archaebacterium Pyrococcus
`furiosus." Appl. Envir. Microbiol. 56, 1992-1998.
`3) Blumentals, I.I., S.H. Brown, R.N. Schicho, A.K. Skaja [Robinson], H.R. Costantino, and R.M.
`Kelly*. (1990) “The Hyperthermophilic Archaebacterium, Pyrococcus furiosus: Development of
`Culturing Protocols, Perspectives on Scale-Up, and Potential Applications.” Ann. N.Y. Acad. Sci.,
`589, 301-314.
`4) Robinson, A.S. and K.D. Wittrup* (1993) “Role of the Protein Folding Chaperone BiP in Secretion
`of Foreign Proteins in Eucaryotic Cells.” in Protein Folding: In vivo and In vitro. ACS
`Symposium Series 526. Jeffrey Cleland, Ed., 121-132.
`5) Robinson, A.S., V. Hines, and K.D. Wittrup*(1994) “Overexpression of Protein Disulfide Isomerase
`Increases Secretion of Foreign Proteins in the Yeast Saccharomyces cerevisiae.” Bio/Tech. 12,
`381-384.
`6) Wittrup*, K.D., A.S. Robinson, R.N. Parekh, and K.J. Forrester (1994) “Existence of an Optimal
`Expression Level for Secretion of Foreign Proteins in Yeast.” Ann. N.Y. Acad. Sci. 745, 321-330.
`7) Robinson, A.S. and K.D. Wittrup*.(1995) “Constitutive Overexpression of Secreted Heterologous
`Proteins Decreases Extractable BiP and PDI Levels in Saccharomyces cerevisiae.” Biotech Prog.
`11, 171-177.
`8) King*, J., C. Haase-Pettingell, A.S. Robinson, M. Speed, and A. Mitraki (1996) “Thermolabile
`Folding Intermediates: Inclusion Body Precursors and Chaperonin Substrates” FASEB J., 10, 57-
`66.
`9) Robinson, A.S., J.A. Bockhaus†, A.C. Voegler, and K.D. Wittrup* (1996) “Reduction of BiP levels
`decreases heterologous protein secretion in Saccharomyces cerevisiae” J. Biol. Chem. 271,
`10017-10022.
`10) Robinson, A.S. and D.A. Lauffenburger* (1996) “Model for ER Chaperone Dynamics and Secretory
`Protein Interactions.” AIChE J. 42, 1443-1453.
`11) Robinson, A.S. and J. King * (1997) “Disulfide-Bonded Intermediate on the Folding and Assembly
`Pathway of a Non-Disulfide Bonded Protein.” Nature Struct. Biol., 4, 450-455.
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`Robinson, A.S.
`12) Foguel, D., Robinson, C.R., Caetano de Sousa Jr., P., Silva, J. L. and A. S. Robinson* (1999),
`“Hydrostatic Pressure Rescues Protein Aggregates”, Biotech. Bioeng. 63, 552-558.
`13) Haase-Pettingell, C., Betts, S., Raso, S.W., Stuart, L., Robinson, A. and J. King* (2001), “Role for
`Cysteine Residues in the In Vivo Folding and Assembly of the Phage P22 Tailspike,” Protein Sci.
`10, 397-410.
`14) Kauffman, K., P. Dhurjati, A.S. Robinson, and F.J. Doyle* III “Framework for Modeling Information
`Flow in Biological Processes: Application to the Unfolded Protein Response.” Proc. IFAC Conf.
`Comput. Appl. Biotech (CAB), 2001.
`15) Kauffman, K., Pridgen, E.M. †, Doyle, F.J. III, Dhurjati, P., and A.S. Robinson* (2002) “Decreased
`Protein Expression and Oscillating BiP Levels Result during Heterologous Protein Expression in
`S. cerevisiae,” Biotech. Prog., 18, 942-940 DOI: 10.1021/bp025518g
`16) Sinacola, J. and A.S. Robinson* (2002) “Rapid refolding and polishing of single-chain antibodies
`from E. coli inclusion bodies” Protein Exp. Purif., Vol. 26, No. 2, Nov 2002, pp. 301-308.
`17) Smith, J.D. and A.S. Robinson* (2002) “Overexpression of an archael enzyme in a eucaryotic host: A
`secretion bottleneck at the ER,” Biotech. Bioeng., 79, 7, p. 713-723. DOI: 10.1002/bit.10367
`18) Lefebvre, B.G., and A.S. Robinson* (2003), “Pressure treatment of tailspike aggregates rapidly
`produces on-pathway folding intermediates,” Biotech. Bioeng, 82, 5, p. 595-604. DOI:
`10.1002/bit.10607
`19) Danek, B.L., and A. S. Robinson* (2003) “Non-native interactions between cysteines direct
`productive assembly of P22 tailspike protein,” Biophys J., 85, 5, p. 1-11.
`20) Butz, J., Niebauer, R. T., and A.S. Robinson* (2003), “Co-expression of molecular chaperones does
`not improve the heterologous expression of mammalian G-protein coupled receptor expression in
`yeast,” Biotech. Bioeng., 84, 3, p. 292-304. DOI: 10.1002/bit.10771.
`21) Gage, M.J. and A.S. Robinson* (2003) “C-terminal Hydrophobic Interactions Play a Critical role in
`Oligomeric Assembly of the P22 Tailspike Trimer,” Protein Sci., 12, 12, p. 2732-47.
`22) Smith, J.D., Tang, B.C. †, and A.S. Robinson* (2004) “Protein disulfide isomerase, but not binding
`protein, overexpression enhances secretion of a non-disulfide-bonded protein in yeast”, Biotech.
`Bioeng., 85, 3, p. 340-50. DOI: 10.1002/bit.10853
`23) Niebauer, R. T. and A.S. Robinson* (2004) “Saccharomyces cerevisiae protein expression: From
`protein production to protein engineering” in Protein Expression Technologies, Horizon Scientific
`Press, François Baneyx, ed.
`24) Lefebvre, B.G., Gage, M.J., and A.S. Robinson* (2004) “Maximizing Recovery of Native Protein
`from Aggregates by Optimizing Pressure Treatment,” Biotechnology Progress, 20, 2, p. 623-629.
`25) Lefebvre, B.G., Comolli, N.K. †, Gage, M.J. and A.S. Robinson* (2004), “Pressure dissociation
`studies provide insight into oligomerization competence of temperature-sensitive mutants of P22
`tailspike,” Protein Sci., 13 (6) 1538-46. DOI: 10.1110/ps.03579304
`26) Danek, B.L. and A. S. Robinson* (2004) “P22 tailspike trimer assembly is governed by interchain
`redox associations,” Biochem. Biophys. Acta, 1700(1):105-16.
`27) Niebauer, R.T., Wedekind, A. † and A.S. Robinson* (2004) “Decreases in yeast expression yields of
`the human adenosine receptor are a result of translational or post-translational events”, Protein
`Exp. Purif., 37 (1) 134-143. DOI: 10.1016/j.pep.2004.06.001
`28) Xu, P., Raden, D., Doyle, F.J. III, and A.S. Robinson* (2005) “Analysis of unfolded protein response
`during single-chain antibody expression in Saccharomyces cerevisiae reveals different roles for
`BiP and PDI in folding”, Metabolic Engineering, 7 (4) 269-279.
`29) Gage, M.J., Zak, J. † and A.S. Robinson* (2005) “Three Amino Acids that are Critical to Formation
`and Stability of the P22 Tailspike Trimer”, Protein Science, 14 (9) 2333-43. DOI:
`10.1110/ps.051394605
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`30) Smith, J.D., Richardson, N.E. and A.S. Robinson* (2005) “Elevated expression temperature in a
`mesophilic host results in increased secretion of a hyperthermophilic enzyme and decreased cell
`stress,” Biochem. Biophys. Acta, 1752 (1) 18-25.
`31) Raden, D., Hildebrandt, S, Xu, P., Bell, E. †, Doyle, III, F.J. and A.S. Robinson* (2005), “Analysis of
`cellular response to protein overexpression.” IEE Proceedings: Systems Biology 152 (4) 285-289.
`PMID: 16986272
`32) Niebauer, R. T., and A.S. Robinson* (2006) “Exceptional total and functional yields of the human
`adenosine (A2a) receptor expressed in the yeast Saccharomyces cerevisiae”, Prot. Exp. Purif., 46,
`p. 204-211.
`33) Gage, M.J, Lefebvre, B.G., and A.S. Robinson* (2006) “Determinants of Protein Folding and
`Aggregation in P22 Tailspike,” in Misbehaving Proteins, ACS Publications, eds. Regina Murphy
`and Amos Tsai.
`34) Kim, J. and Robinson, A.S. * (2006) Dissociation of intermolecular disulfide bonds in P22 tailspike
`protein intermediates in the presence of SDS, Protein Science, 15 (7), p. 1791-3.
`35) Wedekind, A.L. †, O’Malley, M., Niebauer, R.T., and Robinson, A.S. * (2006) Optimization of the
`Human Adenosine A2a Receptor Yields in Saccharomyces cerevisiae, Biotechnology Progress,
`22(5):1249-55. PMID:17022661; DOI: 10.1021/bp050431r
`36) Powers, S.L., Robinson, C.R., and Robinson, A.S. * (2007) Denaturation of an Extremely Stable
`Hyperthermophilic Protein Occurs via a Dimeric Intermediate, Extremophiles, 11(1):179-89.
`37) Forsten-Williams*, K.F., Cassino, T.R, Delo, L.J., Bellis, A.D., Robinson, A.S., and Ryan, T.E.,
`(2007) Enhanced Insulin-like Growth Factor-I (IGF-I) Cell Association at Reduced pH is
`Dependent on IGF Binding Protein-3 (IGFBP-3) Interaction, Journal of Cellular Physiology,
`210(2):298-308.
`38) Famá, M.C., Raden, D., Zacchi, N., Lemos, D.R., Robinson, A.S., and Silberstein, S. * (2007) “The
`Saccharomyces cerevisiae YFR041C/ERJ5 gene encoding a type I membrane protein with a J
`domain is required to preserve the folding capacity of the endoplasmic reticulum” Biochim
`Biophys Acta, 1773(2):232-42.
`39) Bane, S.E., Velasquez, J.E. †, and A.S. Robinson* (2007) “Expression and purification of milligram
`levels of inactive G-protein coupled receptors in E. coli”, Protein Expression and Purification,
`52(2):348:355. PMID: 17166740; DOI: 10.1016/j.pep.2006.10.017
`40) Powers, S.L. and A.S. Robinson* (2007) “PDI Improves Secretion of Redox-Inactive b-glucosidase”,
`Biotech Prog., Mar-Apr;23(2):364-9. E-pub Feb 22, DOI: 10.1021/bp060287p
`41) McCusker, E., Bane, S.E., O’Malley, M., and A.S. Robinson * (2007), “Heterologous GPCR
`expression: A bottleneck to obtaining crystal structures”, Biotech Progress, May-Jun;23(3):540-7.
`PMID:17397185
`42) O’Malley, M., Lazarova, T., Britton, Z.T., and Robinson, A.S. * (2007) “High-level expression in
`Saccharomyces cerevisiae enables isolation and spectroscopic characterization of functional
`human adenosine A2a receptor”, J. Struct Biol., 159(2): 166-178. PMID: 17591446
`43) McCusker, E., and Robinson, A.S.*, (2008) Refolding of G protein α subunits from inclusion bodies
`expressed in Escherichia coli, Protein Exp. Purif., Apr;58(2): 342-55. PMID: 18249008
`44) Hildebrandt, S., D. Raden, L. Petzold, A.S. Robinson, and F.J. Doyle III* (2008) “A top-down
`approach to mechanistic biological modeling: application to the single-chain antibody folding
`pathway”, Biophysical Journal, 95(8):3535-58. Epub 2008 Jul 18. PMID: 18641066
`45) Webber T, Gurung S, Saul J, Baker T, Spatara M, Freyer M, Robinson AS, Gage MJ* (2009) “The C-
`terminus of the P22 tailspike protein acts as an independent oligomerization domain for
`monomeric proteins.”, Biochem J. May 1;419(3):595-602. PMID: 19196242
`46) Spatara ML, Roberts CJ, Robinson AS* (2009) “Kinetic folding studies of the P22 tailspike beta-helix
`domain reveal multiple unfolded states.” Biophys Chem. 141(2-3):214-21. PMID: 19258192
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`47) Xu, P. and Robinson, A.S.* (2009) “Decreased secretion and unfolded protein response up-regulation
`are correlated with intracellular retention for single-chain antibody variants produced in yeast”
`Biotech & Bioeng, 104(1):20-9. PMID: 19415776
`48) O'Malley MA, Mancini JD†, Young CL, McCusker EC, Raden D, Robinson AS*.(2009) “Progress
`toward heterologous expression of active G-protein-coupled receptors in Saccharomyces
`cerevisiae: Linking cellular stress response with translocation and trafficking.” Protein Sci.
`18(11):2356-2370. PMID: 19760666; DOI: 10.1002/pro.246
`49) Spatara, ML and Robinson, AS* (2010) “Transgenic mouse and cell culture models demonstrate a
`lack of mechanistic connection between endoplasmic reticulum stress and tau dysfunction”
`Journal of Neuroscience Research, 88(9):1951-61. PMID: 20143409
`50) O’Malley, MA, AN Naranjo, T Lazarova, AS Robinson* (2010) “Analysis of Adenosine A2a
`Receptor Stability: Effects of Ligands and Disulfide Bonds”, Biochemistry, Nov 2;49(43):9181-9.
`PMID: 20853839
`51) Yuraszeck, TM, Neveu, P, Rodriguez-Fernandez, M, Robinson, AS, Kosik, KS, and FJ Doyle III*
`(2010) Vulnerabilities in the Tau Network and The Role of Ultrasensitive Points in Tau
`Pathophysiology, PLoS Computational Biology, 6(11): e1000997.
`doi:10.1371/journal.pcbi.1000997 (with cover art)
`52) O'Malley, MA, Helgeson, ME, Wagner, NJ and AS Robinson* (2011) Morphology and Composition
`of Cholesterol-rich Micellar Nanostructures Determine Transmembrane Protein (GPCR) Activity,
`Biophysical Journal, 100(2): L11-13. PMID: 3021673.
`53) Sahin, E, JL Jordan, ML Spatara, AN Naranjo, WF Weiss IV, AS Robinson, EJ Fernandez*, CJ
`Roberts* (2011) “Computational Design and Biophysical Characterization of Aggregation-
`Resistant Point Mutations for γD Crystallin Illustrate a Balance of Conformational Stability and
`Intrinsic Aggregation Propensity”, Biochemistry, Feb 8;50(5):628-39. PMID: 21184609
`54) Young CL, Yuraszeck T, Robinson AS* (2011) “Decreased secretion and unfolded protein response
`upregulation,” Methods Enzymol. 491:235-60. PMID: 21329804
`55) Robinson, A.* (2011) “New Tools for Breaking Barriers to GPCR Expression in E. coli” Journal of
`Molecular Biology. 408 (4): 597-598 PMID: 21420418
`56) Britton, Z., Young, C., Can, Ö., McNeely, P., Naranjo, A. and Robinson, A. S.* (2012) Membrane
`Protein Expression in Saccharomyces cerevisiae, in Production of Membrane Proteins: Strategies
`for Expression and Isolation (ed A. S. Robinson), Wiley-VCH Verlag GmbH & Co. KGaA,
`Weinheim, Germany. doi: 10.1002/9783527634521.ch2
`57) O’Malley, Michelle A., Matthew E. Helgeson, Norman J. Wagner, Anne S. Robinson* (2011)
`“Toward Rational Design of Protein Detergent Complexes: Determinants of Mixed Micelles that
`are Critical for the in vitro Stabilization of a G-protein Coupled Receptor”, Biophysical Journal,
`101 (8): 1938-1948. DOI: 10.1016/j.bpj.2011.09.018 PMID: 22004748
`58) Griesemer, M, Young, CL, Doyle III, FJ, Robinson, AS and L Petzold* (2012) Spatial Localization of
`Chaperone Distribution in the Endoplasmic Reticulum of Yeast, IET Systems Biology, 6(2):54-63.
`http://dx.doi.org/10.1049/iet-syb.2011.0006
`59) Young, CL, ZT Britton, AS Robinson* (2012) “Recombinant Protein Expression and Purification: A
`Comprehensive Review of Affinity Tags and Microbial Applications” Biotechnology Journal,
`May;7(5):620-34. doi: 10.1002/biot.201100155. Epub 2012 Jan 10. PMID: 22442034
`60) Young, CL, DL Raden, J Caplan, K Czymmek, AS Robinson* (2012) “Cassette Series Designed for
`Live-Cell Imaging of Proteins and High Resolution Techniques in Yeast”, Yeast, Mar;29(3-
`4):119-36. doi: 10.1002/yea.2895. Epub 2012 Apr 4. PMID: 22473760
`61) Britton, ZT, EI Hanle†, and AS Robinson* (2012) An Expression and Purification System for the
`Biosynthesis of Adenosine Receptor Peptides for Biophysical and Structural Characterization,
`Protein Exp. Purif., 84:224-235. PMID: 22722102; DOI: 10.1016/j.pep.2012.06.005
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`62) McNeely, P.M., Naranjo, A.N., and A.S. Robinson* (2012) Structure-function studies with G-protein
`coupled receptors as a paradigm for improving drug discovery and therapeutic development,
`Biotechnology Journal, 7(12): 1451-1461. DOI: 0.1002/biot.201200076 PMID: 23213015
`63) Young, CL, DL Raden, AS Robinson* (2013) Analysis of ER Resident Proteins in S. cerevisiae:
`Implementation of H/KDEL Retrieval Sequences, Traffic, 14(4):365-81. DOI: 10.1111/tra.12041.
`PMID: 23324027
`64) Morozova, OA, ZM March†, AS Robinson, and DW Colby* (2013) Conformational features of tau
`fibrils from Alzheimer's disease brain are faithfully propagated by unmodified recombinant
`protein, Biochemistry, 52(40):6960-7. http://dx.doi.org/10.1021/bi400866w PMID: 24033133
`65) Maurer, Ronald W., Alan K Hunter, Xiangyang Wang, William K Wang, Anne S Robinson, and
`Christopher J. Roberts* (2013) Folding and aggregation of a multi-domain engineered
`immunotoxin, Biochemical Engineering Journal, 81: 8-14.
`http://dx.doi.org/10.1016/j.bej.2013.09.015.
`66) Blanco, M, Sahin, E, Robinson, A, Roberts, C* (2013) Coarse-Grained Model for Colloidal Protein
`Interactions, B22, and Protein Cluster Formation, The Journal of Physical Chemistry, Part B, Dec
`19;117(50):16013-28. doi: 10.1021/jp409300j PMID: 24289039
`67) Maurer, RW, AK Hunter, AS Robinson*, and CJ Roberts* (2014) Aggregates of alpha-
`chymotrypsinogen anneal to access more stable states, Biotechnology & Bioengineering,
`111 (4): 782-791. doi: 10.1002/bit.25129 PMID: 24122552
`68) St. Amand, MM, Ogunnaike, BA, and Robinson, AS* (2014) Development of At-Line Assay to
`Monitor Charge Variants of mAbs During Production, Biotechnology Progress, 30: 249–255.
`DOI: 10.1002/btpr.1848 PMID: 24382831
`69) St. Amand, MM, K Tran†, D Radhakrishnan, AS Robinson, BA Ogunnaike* (2014) Controllability
`Analysis of Protein Glycosylation in CHO cells, PLoS One, 9(2): e87973. DOI:
`10.1371/journal.pone.0087973
`70) Wu, H, R Kroe-Barrett, S Singh, AS Robinson, CJ Roberts* (2014) Competing aggregation pathways
`for monoclonal antibodies, FEBS Letters 588(6): 936-941.
`http://dx.doi.org/10.1016/j.febslet.2014.01.051
`71) Costanzo, JA, CJ O’Brien, K Tiller†, E Tamargo†, AS Robinson, CJ Roberts, and EJ Fernandez*
`(2013) Conformational stability as a design target to control protein aggregation, Protein Eng,
`Des, & Sel, 27 (5): 157-167. 10.1093/protein/gzu008
`72) St. Amand, MM, D Radhakrishnan, AS Robinson, BA Ogunnaike* (2014) Identification of
`Manipulated Variables for a Glycosylation Control Strategy, Biotech Bioeng, Oct;111(10):1957-
`70. doi: 10.1002/bit.25251.
`73) Griesemer, M., Young, C., Robinson, A.S., Petzold, L.* (2014) BiP Clustering Facilitates Protein
`Folding in the Endoplasmic Reticulum, PLOS Computational Biology, 10(7):1-16,
`10.1371/journal.pcbi.1003675
`74) Young, CL and AS Robinson* (2014) Protein Folding and Secretion: Mechanistic Insights Advancing
`Recombinant Protein Production in S. cerevisiae, Current Opinion in Biotechnology, 30: 168-177.
`DOI: 10.1016/j.copbio.2014.06.018
`75) Naranjo, AN, A Chevalier†, GD Cousins†, E Ayettey†, EC McCusker, C Wenk, AS Robinson* (2015)
`Conserved disulfide bond is not essential for the adenosine A2A receptor: extracellular cysteines
`influence receptor distribution within the cell and ligand-binding recognition, BBA
`Biomembranes, 1848: 603-614 Available on-line Dec 5 2014 DOI:
`10.1016/j.bbamem.2014.11.010
`76) Blocker, KM, ZT Britton, AN Naranjo, PM McNeely, CL Young, AS Robinson* (2015) Recombinant
`G protein-coupled receptor expression in Saccharomyces cerevisiae for protein characterization,
`in “Membrane Proteins – Production and Function Characterization”, Methods Enzymol.,
`556:165-83. doi: 10.1016/bs.mie.2014.12.025
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`77) Wu, H, K Truncali, J Ritchie, R Kroe-Barrett, S Singh, AS Robinson, and CJ Roberts* (2015) Weak
`protein interactions and pH- and temperature-dependent aggregation of human Fc1, mAbs, 7 (6):
`1072-1083 DOI:10.1080/19420862.2015.1079678
`78) O'Brien, C, M Blanco, J Costanzo, M Enterline, EF Fernandez, AS Robinson*, CJ Roberts* (2016)
`Modulating Non-Native Aggregation and Electrostatic Protein-Protein Interactions with
`Computationally Designed Single-Point Mutations, Prot Eng Des & Sel 29 (6): 231-243., doi:
`10.1093/protein/gzw010
`79) Naranjo, AN, PM McNeely, J Katsaras, AS Robinson* (2016) Impact of purification conditions and
`history on A2A adenosine receptor activity: The role of CHAPS and lipids, Prot Exp Purif,
`Aug;124:62-7. DOI: 10.1016/j.pep.2016.05.015
`80) St. Amand, MM, J Hayes, D Radhakrishnan, J Fernandez, B Meyer, AS Robinson, BA Ogunnaike*
`(2016) Identifying a Robust Design Space for Glycosylation during Monoclonal Antibody
`Production, Biotech Prog, Sep;32(5):1149-1162. doi: 10.1002/btpr.2316. Epub 2016 Jul 1.
`81) McNeely, PM, AN Naranjo, KF Williams, and AS Robinson* (2016) A2AR binding kinetics in the
`ligand depletion regime, J. Biomol. Screening, 22 (2): 166-175. DOI:
`10.1177/1087057116667256 PMID: 27577981
`82) Robinson, A.* (2016), Managing and characterizing monoclonal antibody glycosylation. Biotechnol
`Progress, 32: 1089–1090. doi:10.1002/btpr.2387
`83) Wells, EA and AS Robinson* (2016) Cellular engineering for therapeutic protein production: Product
`quality, host modification, and process improvement, Biotechnology J., 12 (1): 1600105. online: 9
`DEC 2016 DOI: 10.1002/biot.201600105
`84) Meric, G, AS Robinson*, CJ Roberts* (2017) Driving Forces for Non-Native Protein Aggregation and
`Approaches to Predict Aggregation-Prone Regions, Annual Review of Chemical and
`Biomolecular Engineering Vol.8: 1, 139-159 DOI: 10.1146/annurev-chembioeng-060816-101404
`85) Radhakrishnan, D., Robinson, A.S. and B. Ogunnaike* (2018) Controlling the glycosylation profile in
`mAbs using time-dependent media supplementation. Antibodies. 7(1), 1;
`https://doi.org/10.3390/antib7010001
`86) O’Brien, C.J., C. Calero-Rubio, V.I. Razinkov, A.S. Robinson*, and C.J. Roberts* (2018) Biophysical
`characterization and molecular simulation of electrostatically driven self-association of a single-
`chain antibody. Protein Science, epub Apr 10. 27 (7): 1275-1285. PMID: 29637646
`DOI: 10.1002/pro.3415
`87) Swonger, K., AS Robinson* (2018) Fluorescence Anisotropy for Determination of Ligand-binding
`Kinetics of Membrane Proteins, Current Protocols in Protein Science, 93, e63.
`doi: 10.1002/cpps.63
`88) Radhakrishnan, D., EA Wells and AS Robinson* (2018) Strategies to enhance productivity and
`modify product quality in therapeutic proteins, Current Opinion in Chemical Engineering, 22: 81-
`88. https://doi.org/10.1016/j.coche.2018.09.005
`89) Jain, A., Stradley, S. and AS Robinson* (2018) The A2aR C-terminus Provides Improved Total and
`Active Expression Yields for Adenosine Receptor Chimeras, AIChE Journal, 64 (12): 4297-4307
`https://doi.org/10.1002/aic.16398
`90) McGraw, C., Yang, L., Levental, I., Lyman,E. and A.S.Robinson* (2019) Membrane cholesterol
`depletion reduces downstream signaling activity of the adenosine A2A receptor, BBA –
` Biomembranes 1861 (4): 760-767 10.1016/j.bbamem.2019.01.001
`
`Under review:
`1) Jain, A., McGraw, C., and A.S. Robinson* (2019) The Adenosine Receptor Carboxy-terminus is
`Necessary for Response but not Specificity of Downstream Signaling, under review.
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`Robinson, A.S.
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`PEER-REVIEWED CONFERENCE PROCEEDINGS
`1) Hildebrandt, S., D. Raden, E. Bell†, A.S. Robinson, and F.J. Doyle III* (2005) “Modeling the
`Unfolded Protein Response in Saccharomyces Cerevisiae”, Proc. Int. Conf. Foundations of
`Systems Biology, Santa Barbara, California. [not indexed]
`2) Griesemer, M., Young, C., Raden, D., Petzold, L., Robinson, A.S., Doyle, F.J. * (2007)
`“Computational Modeling of Chaperone Interactions in the Endoplasmic Reticulum of
`Saccharomyces cerevisiae.” Proc. Int. Conf. Foundations of Systems Biology, Stuttgart, Germany.
`3) Yuraszeck, T., Raden, D, Robinson, A.S., and Doyle, F.J.* (2007) “Microarray Analysis of the
`Unfolded Protein Response in S. cerevisiae Reveals Evidence of Down-regulation.” Proc. Int.
`Conf. Foundations of Systems Biology, Stuttgart, Germany.
`4) Yuraszeck, Theresa M., Pierre Neveu, Maria Rodriguez-Fernandez, Anne Robinson, Kenneth S.
`Kosik, Francis J. Doyle III (2009) Development of a mathematical model to investigate the
`pathophysiology of tau protein.” Proc. Int. Conf. Foundations of Systems Biology, Denver, CO.
`
`
`
`COURSES TAUGHT
`
`University of Delaware, Department of Chemical Engineering
`
`• Mass and Heat Transfer, CHEG 342, ’98-’01, ’05-’07, ’09-’11
`– Undergraduate level, 40-85 students, 2 sections
`– Developed textbook with colleagues TW Fraser Russell and Norman J Wagner, (2008)
`Mass and Heat Transfer: Analysis of Mass Contactors and Heat Exchangers, Cambridge
`University Press, Cambridge, UK (www.cambridge.org/9780521886703) and course
`website www.mht.che.udel.edu
`• Unit Operations Laboratory, CHEG 345, ’02-03
`– Undergraduate level, ~45 students
`– Developed new laboratory module on antibody-antigen interactions
`– Coordinated laboratory course for all students
`• Molecular and Cellular Engineering, CHEG 667 ’98-‘01
`– Undergraduate elective course, ~25 students
`– Developed new course materials
`• Biochemical Engineering, CHEG 620, ’02-’03, ’06, ’09-‘10
`– Undergraduate elective course (required of biochemical minor), ~40 students
`• Multidisciplinary Biotechnology, CHEG/BISC/BUAD 650, ‘04-‘06
`– Graduate elective course, 10-20 students
`– New course implemented as part of IGERT training program
`– Co-taught with Business faculty
`• Biotechnology Lab course, CHEG/CHEM 468, W05, F05
`– Undergraduate course, 5-15 students
`– Co-taught and implemented new biotechnology lab with Chemistry faculty member
`– Sought funding from Engineering and Science Deans to develop course and buy
`equipment
`
`
`
`
`Tulane University, Department of Chemical and Biomolecular Engineering
`• Molecular and Cellular Engineering, S’12, S’13, S’14, F’16
`– Undergraduate/graduate elective course, ~10-20 students
`– Developed new course materials
`• Transport II (Mass and Heat Transfer) F’15
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`Robinson, A.S.
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`– Undergraduate level, ~30 students
`• Graduate Mentoring Seminar F’16, S’17, F’17, F’18
`– Developed material on laboratory safety, publishing and paper writing, and advisor
`selection
`
`
`INVITED SEMINARS
`Department of Chemical Engineering, Worcester Polytechnic Institute, March 2019
`Department of Chemical Engineering, Vanderbilt University, April 2018
`Biophysical Society National Meeting, Bioengineering Subgroup, “The role of cholesterol in
`membrane protein activity”, February 2018
`Bioengineering Program, University of California, Santa Barbara, January 2018
`Department of Chemical and Biomolecular Engineering, Johns Hopkins University, December
`2017
`Department of Chemical Engineering, University of Washington, Seattle, October 2017.
`Department of Chemical & Biomolecular Engineering, University of Houston, September 2017.
`Baton Rouge Community College, March 2017
`Department of Chemical & Biomolecular Engineering, University of Alabama, October 2016.
`CHI GPCR-Based Drug Discovery, Invited Speaker, Cambridge, MA, September 2016
`University of Delaware COBRE Membrane Protein Symposium, Invited Speaker, April 2016
`Department of Chemical Engineering, Purdue University, September, 2015.
`Department of Chemical and Biomolecular Engineering, Clemson University, April, 2015.
`Department of Chemical and Biomolecular Engineering, University of California at Riverside,
`December 2013.
`Department of Chemical and Biomolecular Engineering, The Johns Hopkins University, October
`2013.
`Department of Chemical and Biomolecular Engineering, Texas A&M University, September
`2013.
`Department of Chemical and Biomolecular Engineering, University of Connecticut, April 2013.
`Department of Chemical and Biomolecular Engineering, University of California at Berkeley,
`April 2013.
`Department of Chemistry, University of New Orleans, March 2013
`Department of Biochemistry and Cellular and Molecular Biology Department, University of
`Tennessee, January 2013
`Department of Chemical and Biomolecular Engineering, University of Maryland at College Park,
`January 2013.
`Department of Biochemistry, Tulane University Medical School, December 2012
`Department of Chemical and Biomolecular Engineering, Columbia University, October 2012.
`AS Robinson, “Elucidating Mechanisms of Heterologous GPCR Expression and Trafficking in S.
`cerevisiae” FASEB Molecular Biophysics of Membranes, Snowmass, CO, June 2012
`Department of Chemical and Biomolecular Engineering, University at Buffalo, April 2012.
`Department of Chemistry, Lehigh University, February 2011
`Department of Chemical and Biological Engineering, Rice University, February 2011
`Department of Chemical and Biological Engineering, Northwestern University, December 2010
`Department of Biomedical Engineering, Purdue University, October 2010
`Department of Chemical and Biological Engineering, Rensselaer Polytechnic Institute, April 2010
`Department of Chemical and Biological Engineering, Tufts University, March 2010
`“Understanding Aggregation Pathways and the Benefits of Working Under Pressure”, ACS
`National Meeting, Session in honor of Frederick Heineken, March 2010
`Department of Biological Engineering, Massachusetts Institute of Technology, February 2010
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`“G-Protein coupled receptor expression: Engineering Protein Expression by Understanding
`Biological Interactions”, CHI conference “Membrane Proteins—Extraction, Expression &
`Purification,” San Diego, CA January 2010.
`Department of Chemical and Biological Engineering, University of Wisconsin, October 2009
`“Molecular and Cellular Engineering Strategies for Optimizing Protein Production”, Invited
`Seminar at the New York Academy of Sciences 3rd Annual Advances in Biomolecular
`Engineering: Protein Design Symposium
`(http://www.nyas.org/events/eventDetail.asp?EMSID=471), June 2009
`Department of Chemical and Biomolecular Engineering, Tulane University, March 2009
`Department of Chemical and Biomolecular Engineering, NC State University, November 2008
`Department of Chemical and Biological Engineering, Iowa State University, November 2008
`School of Chemical and Biomolecular Engineering, Georgia Institute of Technology, November
`2007, ACS Progress/Dreyfus Lectureship Award
`Department of Chemical Engineering, University of South Carolina, April 2007
`Department of Chemical Engineering, University of Ma