I 1111111111111111 11111 1111111111 111111111111111 IIIII 11111 lll111111111111111
`
`US008933215B2
`
`c12) United States Patent
`Giese et al.
`
`(IO) Patent No.:
`(45) Date of Patent:
`
`US 8,933,215 B2
`*Jan. 13, 2015
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`FOREIGN PATENT DOCUMENTS
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`8/2003
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`196 18 797
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`02018572.4
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`(Continued)
`
`OTHER PUBLICATIONS
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`(54)
`
`INTERFERING RNA MOLECULES
`
`(71) Applicant: Silence Therapeutics
`Aktiengesellschaft (AG), Berlin (DE)
`
`(72)
`
`Inventors: Klaus Giese, Berlin (DE); Jiirg
`Kaufmann, Berlin (DE); Anke
`Klippel-Giese, Berlin (DE)
`
`(73) Assignee: Silence Therapeutics
`Aktiengesellschaft (AG), Berlin (DE)
`
`( *) Notice:
`
`Subject to any disclaimer, the term ofthis
`patent is extended or adjusted under 35
`U.S.C. 154(b) by O days.
`
`This patent is subject to a terminal dis(cid:173)
`claimer.
`
`(21) Appl. No.: 13/692,178
`
`(22) Filed:
`
`Dec. 3, 2012
`
`(65)
`
`Prior Publication Data
`
`US 2013/0102769 Al
`
`Apr. 25, 2013
`
`Related U.S. Application Data
`
`5,514,546 A
`5,589,389 A
`5,670,633 A
`5,792,847 A
`5,872,232 A
`5,898,031 A
`5,914,396 A
`5,955,589 A
`6,005,087 A
`6,107,094 A
`6,129,912 A
`6,153,737 A
`6,165,772 A
`6,239,272 Bl
`6,284,267 Bl
`6,476,205 Bl
`6,506,559 Bl
`6,531,584 Bl
`6,617,438 Bl
`6,812,204 Bl
`7,056,704 B2
`7,074,863 B2
`7,078,196 B2
`7,138,517 B2
`7,148,342 B2
`7,196,184 B2
`7,348,314 B2
`2003/0027783 Al
`2003/0108923 Al
`2003/0135033 Al
`2003/0190635 Al
`2004/0053875 Al
`
`(63) Continuation of application No. 12/986,389, filed on
`Jan. 7, 2011, now Pat. No. 8,324,370, which is a
`continuation of application No. 12/200,296, filed on
`Aug. 28, 2008, now Pat. No. 7,893,245, which is a
`continuation of application No. 10/633,630, filed on
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`
`(60) Provisional application No. 60/402,541, filed on Aug.
`12, 2002.
`
`CA
`DE
`EP
`EP
`EP
`
`(30)
`
`Foreign Application Priority Data
`
`Aug. 5, 2002
`Apr. 10, 2003
`
`(EP) ..................................... 02017601
`(EP) ..................................... 03008383
`
`(51)
`
`(2006.01)
`(2010.01)
`(2006.01)
`(2006.01)
`
`Int. Cl.
`C07H 21104
`C12N 151113
`C12N 15111
`A61K 38/00
`(52) U.S. Cl.
`CPC ........ C12N 151113 (2013.01); Cl2N 2310/346
`(2013.01); Cl2N 2310/53 (2013.01); C12N
`151111 (2013.01); Cl2N 2310/3183 (2013.01);
`Cl2N 2310/14 (20l3.01);A61K 38/00
`(2013.01); Cl2N 2310/321 (2013.01); Cl2N
`2320/51 (2013.01)
`USPC ......... 536/24.5; 536/24.1; 536/24.31; 514/44
`( 58) Field of Classification Search
`None
`See application file for complete search history.
`
`(56)
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`(Continued)
`
`Primary Examiner - Kimberly Chong
`(74) Attorney, Agent, or Firm - Saliwanchik, Lloyd &
`Eisenchenk
`
`(57)
`
`ABSTRACT
`
`The present invention is related to a ribonucleic acid com(cid:173)
`prising a double stranded structure whereby the double(cid:173)
`stranded structure comprises a first strand and a second
`strand, whereby the first strand comprises a first stretch of
`contiguous nucleotides and whereby said first stretch is at
`least partially complementary to a target nucleic acid, and the
`second strand comprises a second stretch of contiguous
`nucleotides whereby said second stretch is at least partially
`identical to a target nucleic acid, and whereby the double
`stranded structure is blunt ended.
`
`19 Claims, 29 Drawing Sheets
`
`Alnylam Exh. 1023
`
`i
`
`

`

`US 8,933,215 B2
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