I 1111111111111111 11111 lllll lllll lllll lllll 111111111111111 111111111111111111
`US0083243 70B2
`
`c12) United States Patent
`Giese et al.
`
`(IO) Patent No.:
`(45) Date of Patent:
`
`US 8,324,370 B2
`*Dec. 4, 2012
`
`(54)
`
`INTERFERING RNA MOLECULES
`
`(75)
`
`Inventors: Klaus Giese, Berlin (DE); Jiirg
`Kaufmann, Berlin (DE); Anke
`Klippel-Giese, Berlin (DE)
`
`(73) Assignee: Silence Therapeutics
`Aktiengesellschaft (AG), Berlin (DE)
`
`( *) Notice:
`
`Subject to any disclaimer, the term ofthis
`patent is extended or adjusted under 35
`U.S.C. 154(b) by O days.
`
`This patent is subject to a terminal dis(cid:173)
`claimer.
`
`(21) Appl. No.: 12/986,389
`
`(22) Filed:
`
`Jan.7,2011
`
`(65)
`
`Prior Publication Data
`
`US 2011/0118456 Al
`
`May 19, 2011
`
`Related U.S. Application Data
`
`(63) Continuation of application No. 12/200,296, filed on
`Aug. 28, 2008, now Pat. No. 7,893,245, which is a
`continuation of application No. 10/633,630, filed on
`Aug. 5, 2003, now Pat. No. 7,452,987.
`
`(60) Provisional application No. 60/402,541, filed on Aug.
`12, 2002.
`
`(30)
`
`Foreign Application Priority Data
`
`Aug. 5, 2002
`Apr. 10, 2003
`
`(EP) ..................................... 02017601
`(EP) ..................................... 03008383
`
`(51)
`
`Int. Cl.
`(2006.01)
`C07H 21104
`(52) U.S. Cl. ................... 536/24.5; 536/24.31; 536/24.1;
`514/44; 435/6; 435/325; 435/375
`(58) Field of Classification Search ........................ None
`See application file for complete search history.
`
`(56)
`
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`(Continued)
`
`Primary Examiner - Kimberly Chong
`(74) Attorney, Agent, or Firm - Saliwanchik, Lloyd &
`Eisenschenk
`
`(57)
`
`ABSTRACT
`
`The present invention is related to a ribonucleic acid com(cid:173)
`prising a double stranded structure whereby the double(cid:173)
`stranded structure comprises a first strand and a second
`strand, whereby the first strand comprises a first stretch of
`contiguous nucleotides and whereby said first stretch is at
`least partially complementary to a target nucleic acid, and the
`second strand comprises a second stretch of contiguous
`nucleotides whereby said second stretch is at least partially
`identical to a target nucleic acid, and whereby the double
`stranded structure is blunt ended.
`
`23 Claims, 29 Drawing Sheets
`
`Alnylam Exh. 1022
`
`i
`
`

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`US 8,324,370 B2
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