111111
`
`1111111111111111111111111111111111111111111111111111111111111
`US009222092B2
`
`c12) United States Patent
`Giese et al.
`
`(10) Patent No.:
`(45) Date of Patent:
`
`US 9,222,092 B2
`*Dec. 29, 2015
`
`(54)
`
`INTERFERING RNA MOLECULES
`
`(56)
`
`References Cited
`
`(71) Applicant: SILENCE THERAPEUTICS
`AKTIENGESELLSCHAFT (AG),
`Berlin (DE)
`
`(72)
`
`Inventors: Klaus Giese, Berlin (DE); Jiirg
`Kaufmann, Berlin (DE); Anke
`Klippel-Giese, Berlin (DE)
`
`(73) Assignee: SILENCE THERAPEUTICS GMBH,
`Berlin (DE)
`
`( *) Notice:
`
`Subject to any disclaimer, the term of this
`patent is extended or adjusted under 35
`U.S.C. 154(b) by 0 days.
`
`This patent is subject to a terminal dis(cid:173)
`claimer.
`
`(21) Appl. No.: 14/578,636
`
`(22) Filed:
`
`Dec. 22, 2014
`
`(65)
`
`Prior Publication Data
`
`US 2015/0105545 Al
`
`Apr. 16, 2015
`
`Related U.S. Application Data
`
`(63) Continuation of application No. 13/692,178, filed on
`Dec. 3, 2012, now Pat. No. 8,933,215, which is a
`continuation of application No. 12/986,389, filed on
`Jan. 7, 2011, now Pat. No. 8,324,370, which is a
`continuation of application No. 12/200,296, filed on
`Aug. 28, 2008, now Pat. No. 7,893,245, which is a
`continuation of application No. 10/633,630, filed on
`Aug. 5, 2003, now Pat. No. 7,452,987.
`
`(60) Provisional application No. 60/402,541, filed on Aug.
`12,2002.
`
`(30)
`
`Foreign Application Priority Data
`
`Aug. 5, 2002
`Apr. 10, 2003
`
`(EP) ..................................... 02017601
`(EP) ..................................... 03008383
`
`(51)
`
`(2006.01)
`(2010.01)
`(2006.01)
`(2006.01)
`
`Int. Cl.
`C07H 21104
`C12N 151113
`C12N 15111
`A61K 38/00
`(52) U.S. Cl.
`CPC .......... C12N 1511137 (2013.01); C12N 151111
`(2013.01); C12N 151113 (2013.01); A61K
`38/00 (2013.01); C12N 2310/14 (2013.01);
`C12N 2310/3183 (2013.01); C12N 2310/321
`(2013.01); C12N 2310/346 (2013.01); C12N
`2310/53 (2013.01); C12N 2310/531 (2013.01);
`C12N 2320/51 (2013.01)
`(58) Field of Classification Search
`None
`See application file for complete search history.
`
`U.S. PATENT DOCUMENTS
`
`4,217,294 A
`5,466,786 A
`5,514,546 A
`5,589,389 A
`5,670,633 A
`5,792,847 A
`5,872,232 A
`5,898,031 A
`5,914,396 A
`5,955,589 A
`6,005,087 A
`6,107,094 A
`6,129,912 A
`6,153,737 A
`6,165,772 A
`6,239,272 B1
`6,284,267 B1
`6,476,205 B1
`6,506,559 B1
`6,531,584 B1
`6,617,438 B1
`6,812,204 B1
`7,056,704 B2
`7,074,863 B2
`7,078,196 B2
`7,138,517 B2
`7,148,342 B2
`7,196,184 B2
`7,348,314 B2
`2003/0027783 A1
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`
`FOREIGN PATENT DOCUMENTS
`
`CA
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`8/2003
`2476112
`1111997
`196 18 797
`(Continued)
`OTHER PUBLICATIONS
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`U.S. Appl. No. 60/352,873, filed Feb. 1, 2002, Darnha, M. J. eta!.
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`(Continued)
`
`Primary Examiner- Kimberly Chong
`(74) Attorney, Agent, or Firm- Saliwanchik Lloyd &
`Eisenschenk
`ABSTRACT
`(57)
`The present invention is related to a ribonucleic acid com(cid:173)
`prising a double stranded structure whereby the double(cid:173)
`stranded structure comprises a first strand and a second
`strand, whereby the first strand comprises a first stretch of
`contiguous nucleotides and whereby said first stretch is at
`least partially complementary to a target nucleic acid, and the
`second strand comprises a second stretch of contiguous
`nucleotides whereby said second stretch is at least partially
`identical to a target nucleic acid, and whereby the double
`stranded structure is blunt ended.
`10 Claims, 29 Drawing Sheets
`
`Alnylam Exh. 1024
`
`i
`
`

`

`US 9,222,092 B2
`Page 2
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