(12) United States Patent
`US 10,533,183 B2
`(10) Patent No.:
`(45) Date of Patent:
`*Jan. 14, 2020
`Cirpus et al.
`
`US010533183B2
`
`(54) OILS, LIPIDS AND FATTY ACIDS
`PRODUCED IN TRANSGENIC BRASSICA
`PLANT
`
`_
`.
`(71) Appl1cant: BASF Plant Science GmbH,
`Ludwigshafen (DE)
`
`(72)
`
`Inventors: Petra Cirpus, Mannheim (DE); Jorg
`Bauer, L1mburgerhof (DE); Xiao Qiu,
`Saskatoon (CA); Guohai Wu,
`Saskatoon (CA); Bifang Cheng,
`.
`-
`saSkatoon (CA), Mam“ Tfuksa’
`SaskatOQIl (CA); T0111 WetJena
`Mannhelm (DE)
`
`.
`.
`.
`(73) A551gnee. BASF Plant Science GmbH,
`Ludw1gshafen (DE)
`
`>1:
`
`.
`~
`) Notlce.
`
`(
`
`~
`~
`~
`~
`Subject. to any dlsclalmer, the term ofthis
`patent 15 extended or adjusted under 35
`U.S.C. 154(b) by 0 days.
`This patent is subject to a terminal dis-
`claimer.
`
`(21) APP1~ N05 16/371337
`
`(22)
`
`Filed:
`
`Apr. 1, 2019
`
`(65)
`
`Prior Publication Data
`
`US 2019/0218568 A1
`
`Jul. 18, 2019
`
`Related US Application Data
`(63) Cont1nuat1on of appl1catlon No. 15/256,9l4, filed on
`Sep. 9, 2016, which is a continuation of application
`No.
`12/280,090,
`filed
`as
`application No.
`PCT/EP2007/051675 on Feb. 21, 2007, now Pat. No.
`10,190,131.
`
`(30)
`
`Foreign Application Priority Data
`
`Feb. 21, 2006
`Sep. 7, 2006
`
`(DE) ........................ 10 2006 008 030
`(EP) ..................................... 06120309
`
`(51)
`
`(2006.01)
`(2006.01)
`(2006.01)
`(2006.01)
`(2006.01)
`(2006.01)
`
`Int. Cl.
`A61K 31/20
`C12N 15/82
`C12N 9/02
`C12N 9/10
`C12N 15/52
`CUP 7/64
`(52) US. Cl.
`CPC ....... C12N 15/824 7 (2013.01); C12N 9/0071
`(2013.01); C12N 9/0083 (2013.01); C12N
`9/1029 (2013.01); C12N 15/52 (2013.01);
`C12P 7/6427 (2013.01); CI2Y 114/19
`(2013.01)
`
`(56)
`
`References Cited
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`(Continued)
`
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`
`AU
`AU
`
`ll/2003
`2003232512 Al
`1/2004
`2003258496 A1
`(Continued)
`
`OTHER PUBLICATIONS
`
`Us’ APPI' NO' 60/613,861, Smgh et 31'
`(Continued)
`
`Primary Examiner 7 Hope A Robinson
`(74) Attorney, Agent, or Firm A Drinker Biddle & Reath
`LLP
`
`(57)
`
`ABSTRACT
`
`The invention relates to a method for producing eicosapen-
`tanoic acid, docosapentanoic acid and/or docohexanoic acid
`in transgenic plants. According to said method, the plant is
`provided with at least one nucleic acid sequence coding for
`a polypeptide with a A6 desaturase activity, at least one
`nucleic acid sequence coding for a polypeptide with a A6
`elongase activity, at least one nucleic acid sequence coding
`for a polypeptide with a A5 desaturase activity, and at least
`one nucleic acid sequence coding for a polypeptide with a
`A5 elongase activity, the nucleic acid sequence coding for a
`polypeptide with a A5 elongase activity being modified in
`relation to the nucleic acid sequence in the organism from
`which the sequence originates, such that it is adapted to the
`codon use in at least one type of plant. For the production of
`docosahexanoic acid, at
`least one nucleic acid sequence
`coding for a polypeptide with a A4 desaturase activity is also
`introduced into the plant.
`
`(58) Field of Classification Search
`None
`
`9 Claims, No Drawings
`
`See application file for complete search history.
`
`Specification includes a Sequence Listing.
`
`1 of 37
`10f37
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`CSIRO Exhibit 1001
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`

`

`US 10,533,183 B2
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