iN
`
`
`TELE, WNTAD SUATES; OL}
`
`Whereas, THERE HAS BEEN PRESENTED 0 THE
`
`
`
`
`NO)ALi,LOWHOMTHESE; PRESENTS SHAT, COME
`Pioneer Hi-Bred International, Inc.
`
`
`
`Secretary of Agriculture
`
`an alleged distinct variety of sexually
`An application requesting a certificate of protection for
`are contained in the |
`reproduced, or tuber propagated plant,
`the name and description of which
`application and exhibits, a copy of whichis hereunto annexed and made a part hereof, and the various
`ji
`
`requirements of LAWin such cases made and provided have been complied with, andthe title thereto Hk
`
`is, from the records of the PLANT VARIETY PROTECTION OFFICE, in the applicant(s) indicated in the said |
`
`
`and Whereas,upon due examination made, the said applicant(s) is (are) adjudged to be entitled
`:
`copy,
`
`to a certificate of plant variety protection underthe LAW.
`
`
`Now,therefore, this certificate of plant variety protection is to grant unto the said applicant(s) and the | i
`
`successors, heirs orassigns of the said applicant(s) for the term of TWENTYyears fromthe date of this
`
`grant, subject to the paymentof the required fees and periodic replenishment of viable basic seed of the
`as provided by LAW,theright to exclude others from selling the variety,
`§ Be
`
`
`variety in a public repository
`
`or offering it for sale, or reproducing it, or
`importing it, or exporting it, or conditioning it for
`
`propagation, or stocking it for any of the above purposes, orusingit in producing a hybrid ordifferent
`variety therefrom, to the extent provided by the PLANT VARIETY PROTECTION ACT. (84 STAT. 1542, AS |
`
`
`AMENDED,7 U.S.C. 2321 ET SEQ.)
`x ;
`
`
`a
`CORN, FIELD
`
`'PHI8KJ!
`
`Attest: =ee
`
`
`
`
`
`
`
`
`
`
`)
`
`
`In Testimony Whereof, J have hereunto set my
`hand and caused the seal of the Plant Variety
`
`Protection Office to be affixed at the City of
`
` of |\y
`Washington, D.C.
`this
`thirtieth
`day
`
`September,
`in the year two thousand and
`
`thirteen.
`
`eI aw®
`
`
`Secretaryof Ags
`
`Commissioner
`Plant Variety Protection Office
`Agriculiural Marketing Service
`
`EX1009 - Page 1
`EX1009 - Page 1
`
`
`
`
`
`
`
`Inari Exhibit 1009
`Inari Exhibit 1009
`Inari v. Pioneer
`Inari v. Pioneer
`
`

`

`Brad Hall, Senior Research Associate
`Pioneer Hi-Bred International, Inc.
`Crop Genetics Research and Development
`7301 NW 62nd Avenue, PO Box 85
`Johnston, IA 50131-0085
`.
`.
`515-535-6975
`
`
`
`
`
`515-535-2125
`
`
`
`
`
`
`
`EX1009 - Page 2
`
`JMS
`
`

`

`EX1009 - Page 3
`EX1009 - Page 3
`
`

`

`. ' GE;N~RA~ INSTRUCTIONS:
`,p,VPO:
`To be effectively filed with the Plant Variety Protecti.on Office (PVPO), ALL of the following items must be receiveddn:ttle
`: ,(1) 9.~iLipleted application form signed by the owner;
`(2) completed exhibits A, B, C, E, F;
`(3) for a tuber
`reproduced variety, verification that a vlaj:JIe,(in.the
`tissue culture will be deposited and maintained in an approved public repository; and (4) payment by c.redit,card;or,
`sense that it will reproduce an entire plant)
`check drawn on a U.S. bank for $4,382 ($518 filing fee and $3,864 examination fee), payable to "Treasurer of the United States"
`(See Section 97:6 otthe
`..
`Regulations and Rules of Practice). NEW: With the application for a seed reproduced variety or by direct deposit
`soon after
`filing,
`the applicant must provide
`at least 3,000 viable untreated seeds of the variety per se, and for a hybrid variety at least 3,000 untreated seeds of each line necessary to reproduce
`the variety.
`Partial applications will be held in the PVPO for not more than 90 days;
`then returned to the applicant as un-filed. Mail application and other requirements
`to'Plant
`Variety Protection Office, AMS, USDA, Room 401, NAL Building, 10301 Baltimore Avenue, Beltsville, MD 20705-2351. Retain one copy for your files. All
`items
`on the face of the application are self explanatory unless noted below. Corrections on the application form and exhibits must be initialed and dated. DO NOT use
`masking materials to make corrections.
`If a certificate is allowed, you will be requested to send a payment by credit card or check payable to "Treasurer of the
`United States"
`in the amount of $768 for issuance of the certificate. Certificates will be issued to owner, not licensee or agent.
`
`to keep the PVPO informed of any changes of address or change of ownership or assignment or owner's
`It is the responsibility of the applicant/owner
`NOTES:
`representative during the life of the application/certificate.
`The fees for filing a change of address; owner's representative;
`ownership or assignment; or any
`modification of owner's name is specified in Section 97.175 of the regulations.
`(See Section 101 of the Act, and Section~/97.130,
`97.131, 97. 175(h) ofthe
`Regulations and Rules of Practice.)
`
`1T 2 0 1 0 0 0 3 6 6
`
`Plant Variety Protection Office
`Telephone:
`(301) 504-5518
`General E-mail: PVPOmail@usda.gov
`Homepage:
`http://www.ams.usda.gov/science/pvpo/PVPindex.htm
`
`FAX: (301) 504-5291
`
`SPECIFIC INSTRUCTIONS:
`To avoid conflict with other variety names in use, the applicant must check the appropriate recognized authority and provide evidence that the permanent name
`of the application variety (even if it is a parental,
`inbred line) has been cleared by the appropriate recognized authority before the Certificate of Protection is
`issued. For example,
`for agricultural and vegetable crops, contact: U.S. Department of Agriculture, Agricultural Marketing Service, Livestock and Seed
`Programs, Seed Regulatory
`and Testing Branch, 801 Summit Crossing Place, Suite C, Gastonia, North Carolina 28054-2193 Telephone:
`(704) 810-8870.
`http://www.ams.usda.gov/lsg/seed.htm.
`
`ITEM
`19a. Give:
`
`lines, or clones used, and the breeding method;
`including public and commercial varieties,
`the genealogy,
`(1)
`the details of subsequent stages of selection and multiplication;
`(2)
`(3) evidence of uniformity and stability; and
`(4)
`the type and frequency of variants during reproduction and multiplication and state how these variants may be identified
`
`19b. Give a summary of the variety's distinctness. Clearly state how this application variety may be distinguished from all other varieties in the same crop.
`new variety is most similar
`to one variety or a group of related varieties:
`
`If the
`
`identify these varieties and state all differences objectively;
`(1)
`(2) attach replicated statistical data for characters expressed numerically and demonstrate that these are clear differences; and
`(3) submit,
`if helpful, seed and plant specimens or photographs
`(prints) of seed and plant comparisons which clearly indicate distinctness.
`
`19c. Exhibit C forms are available from the PVPO Office for most crops; specify crop kind. Fill
`as possible to describe your variety.
`
`in Exhibit C (Objective Description of Variety)
`
`form as completely
`
`that cannot be accurately conveyed in Exhibit C. Use
`and/or photographs. Describe any additional characteristics
`19d. Optional additional characteristics
`comparative varieties as is necessary to reveal more accurately the characteristics
`that are difficult
`to describe, such as plant habit, plant color, disease
`resistance, etc.
`
`1ge. Section 52(5) of the Act requires applicants to furnish a statement of the basis of the applicant's ownership. An Exhibit E form is available from the PVPO.
`
`20.
`
`the applicant MAY NOT reverse this affirmative decision
`If "Yes" is specified (seed of this variety be sold by variety name only, as a class of certified seed),
`after the variety has been sold and so labeled,
`the decision published, or the certificate issued. However,
`if "No" has been specified,
`the applicant may
`change the choice.
`(See Regulations and Rules of Practice, Section 97.103).
`
`23. See Sections 41, 42, and 43 of the Act and Section 97.5 ofthe regulations for eligibility requirements.
`
`24. See Section 55 of the Act
`
`for instructions on claiming the benefit of an earlier
`
`filing date.
`
`22. CONTINUED FROM FRONT
`
`(Please provide a statement as to the limitation and sequence of generations
`
`that may be certified.)
`
`transfer, or use for each country and the circumstances,
`(Please provide the date of first sale, disposition,
`23. CONTINUED FROM FRONT
`if the variety
`(including any harvested material) or a hybrid produced from this variety has been sold, disposed of, transferred, or used in the U.S. or other countries.)
`
`(Please give the country, date of filing or issuance, and assigned reference number,
`24. CONTINUED FROM FRONT
`(Plant Breeder's Right or Patent).)
`variety is protected by intellectual property right
`
`if the variety or any component of the
`
`USPTO filing date 5/25/2010, Application No. 12/786,571
`
`to the Paperwork Reduction Act of 1995, an agency may not conduct or sponsor, and a person is not required to respond to a collection of information
`unless it displays a valid OMB control number.
`According
`1.4 hours per
`valid OMB control number
`for this information
`collection is 0581-0055.
`The time required to complete
`this information
`collection is estimated
`to average
`response,
`including
`the time for reviewing
`instructions,
`searching
`existing data sources, gathering
`and maintaining
`the data needed, and completing
`and reviewing the collection of information.
`
`The
`
`familial status,
`sex, marital status,
`and activities on the basis of race, color, national origin, age, disability, and where applicable,
`its programs
`in all
`(USDA) prohibits discrimination
`of Agriculture
`The U.S. Department
`reprisal, or because all or part of an individual's
`income is derived from any public assistance
`parental
`status,
`religion,
`sexual orientation,
`genetic information,
`political beliefs,
`bases apply
`program (Not all prohibited
`to all programs.)
`Persons with disabilities who require alternative means for communication
`of program information
`(Braille,
`large print, audiotape,
`etc.) should contact USDA's TARGET Center at (202) 720-2600
`(voice and TOO). To file a complaint
`or call (800) 795-3272
`(voice) or (202) 720-
`Avenue, S. W, Washington, D. C. 20250-9410,
`of discrimination,
`write to USDA, Director, Office of Civil Rights, 1400 Independence
`6382 (TOO). USDA is an equal opportunity
`provider and employer.
`
`ST-470
`
`(07-01-2009)
`
`designed
`
`by the Plant Variety Protection OffICe
`
`EX1009 - Page 4
`
`

`

`#201000366
`
`Exhibit A: Origin and Breeding History for PH18KJ
`
`by Pioneer Hi-Bred
`line PH18KJ, Zea mays L., a yellow corn inbred, was developed
`Pioneer
`International,
`Inc. from the single cross hybrid PH8JR (PVP Certificate No. 200400191) X PHB 1V
`(PVP Certificate No. 200400201)
`using the pedigree
`selection method of plant breeding. Varieties
`PH8JR and PHB 1V are proprietary
`inbred lines of Pioneer Hi-Bred International,
`Inc. Selfing was
`practiced
`from above hybrid for 7 generations
`using pedigree
`selection. During line development,
`crosses were made to inbred testers
`for the purpose of estimating
`the line's combining
`ability. Yield
`trials were grown at Windfall,
`IN, USA as well as other Pioneer
`research locations. After
`initial
`testing,
`additional
`hybrid combinations
`have been evaluated
`and subsequent
`generations
`of the line have been
`grown and hand-pollinated
`with observations
`again made for uniformity.
`
`in Exhibit C - "Objective
`and stability for all traits as described
`Variety PH18KJ has shown uniformity
`Description
`of Variety."
`It has been self-pollinated
`and ear rowed for 5 generations with careful
`attention paid to selection criteria and uniformity
`of plant
`type to assure the variety is genetically
`homozygous
`and phenotypically
`stable. The line has been increased
`both by hand and in isolated fields
`with continued
`observations
`for uniformity
`and stability,
`and for 6 generations
`during the final stages
`of inbred development
`and seed multiplication.
`Very high standards
`for genetic purity have been
`established morphologically
`using field observations
`and using sound laboratory methodologies.
`
`No variant
`
`traits have been observed or are expected in PH18KJ.
`
`The criteria used in the selection of PH 18KJ were yield, both per se and in hybrid combinations.
`season plant health, grain quality, and stalk lodging resistance, were important
`criteria considered
`during selection. Other selection criteria include:
`ability to germinate
`in adverse conditions,
`disease and
`insect
`resistance,
`pollen yield and tassel size.
`
`Late
`
`Exhibit A: Developmental History for PH18KJ
`
`Pedigree
`PH8JR/PHB 1V
`PH8JR/PHB 1V)X
`PH8JR/PHB 1V)X6
`PH8JR/PHB 1V)X63
`PH8JR/PHB 1V)X634
`PH8JR/PHB 1V)X6341
`PH8JR/PHB 1V)X63413
`PH8JR/PHB 1V)X634132
`PH8JR/PHB1 V)X634132X
`
`Year Grown
`2002
`2004
`2005
`2006
`2006
`2007
`2007
`2008
`
`Generation
`F1
`F2
`F3
`F4
`F5
`F6
`F7
`F8
`F9 (Seed)
`
`and stability were
`from F3 through F8 generation. Uniformity
`PH18KJ was selfed and ear-rowed
`established
`from F4 through F8 generation
`and beyond when seed supplies were increased.
`
`EX1009 - Page 5
`
`

`

`#201000366
`
`Exhibit B: Novelty Statement
`
`Inc. proprietary inbred line PH24 E
`Variety PH 18KJ mostly resembles Pioneer Hi-Bred International,
`(PVP Certificate No. 9600204). Table 1 shows two sample t-tests on data collected primarily in
`Johnston and Dallas Center, Iowa in 2009. The traits collectively show measurable differences between
`the two varieties.
`
`Variety PH18KJ has a wider cob diameter (26.4 mm vs. 24.3 mm) than variety PH24E (Table 1).
`
`Variety PH18KJ has a shorter ear length (13.6 cm vs. 16.1 cm) than variety PH24E (Table 1).
`
`Variety PH18KJ has more leaves above top ear (5.8 vs. 4.6) than variety PH24E (Table 1).
`
`In addition to the differences cited above, the database records indicate 'PH18KJ' is most similar
`to 'PH24E'; however, 'PH18KJ' differs from 'PH24E' in glume color (red - 10RP 3/10 vs. light
`green – 7.5GY 5/8 Munsell, respectively).
`
`MARK HERMELING
`__________________________________ Mark A. Hermeling 6-27-2013
`
`Digitally signed by MARK HERMELING
`DN: c=US, o=U.S. Government, ou=Department
`of Agriculture, cn=MARK HERMELING,
`0.9.2342.19200300.100.1.1=12001000112718
`Date: 2013.06.28 07:13:26 -05'00'
`
`EX1009 - Page 6
`
`

`

`6.2
`
`5.3
`
`5.8
`
`18
`
`9
`
`27
`
`Cnt-2Mean-1Mean-2Mean_Oiff
`
`16.7
`
`15.0
`
`16.1
`
`14.2
`
`13.1
`
`13.6
`
`18
`
`9
`
`27
`
`Cnt-2Mean-1Mean-2Mean_Oiff
`
`25.3
`
`22.3
`
`24.3
`
`27.0
`
`25.7
`
`26.4
`
`18
`
`9
`
`27
`
`Cnt-2Mean-1Mean-2Mean_Oiff
`
`18
`
`18
`
`36
`Cnt-1
`
`18
`
`18
`
`36
`Cnt-1
`
`18
`
`18
`
`36
`Cnt-1
`
`PH24E
`
`PH24E
`
`PH24E
`Variety-2
`
`PH24E
`
`PH24E
`
`PH24E
`Variety-2
`
`PH24E
`
`PH24E
`
`PH24E
`Variety-2
`
`PH18KJ
`
`PH18KJ
`
`PH18KJ
`Variety-1
`
`PH18KJ
`
`PH18KJ
`
`PH18KJ
`Variety-1
`
`PH18KJ
`
`PH18KJ
`
`PH18KJ
`Variety-1
`
`2009
`Year
`
`2009
`Year
`
`2009
`Year
`
`JHNAPN01-2009
`
`DSBNITDS-2009
`
`3.Environ.
`
`3.Environ.
`
`2.Year
`Level
`----------------------------------------------
`Leafnumberabovetopear
`
`Location
`
`JHNAPN01-2009
`
`DSBNITDS-2009
`
`3.Environ.
`
`3.Environ.
`
`2.Year
`Level
`----------------------------------------------
`Earlength(em)
`
`Location
`
`JHNAPN01-2009
`
`DSBNITDS-2009
`
`3.Environ.
`
`3.Environ.
`
`EX1009 - Page 7
`
`2.Year
`Level
`----------------------------------------------
`Cobdiameter
`
`Location
`
`(mm)
`
`differentplantingdatesandwereindifferentfields.Atwo-samplet-testwasusedtocomparedifferencesbetweenmeans.
`evidencefordifferencesbetweenPH18KJandPH24E.Varietiesweregrownintwolocationsthathaddifferentenvironmentalconditions.Environmentshad
`Table1:DatafromJohnstonandDallasCenter,Iowain2009presentedbytrait,acrossenvironments,andbrokenoutbyenvironment.Dataaresupporting
`
`ExhibitB:NoveltyStatementTable
`
`PH1BKJ_PH24E
`Table1
`
`0)
`
`)
`000 ~0
`
`....Jl.
`
`0.
`
`',)
`
`~i
`
`0.000
`
`0.000
`
`6.5
`
`4.5
`
`34
`
`25
`
`0.121
`
`0.200
`
`0.167
`
`0.162
`
`0.514
`
`0.601
`
`0.707
`
`0.686
`
`Prob_Pool
`
`t-Value
`
`OF
`
`StErr-2
`
`StErr-1
`
`StDev-2
`
`StDev-1
`
`0.000
`
`0.000
`
`-7.0
`
`-5.0
`
`34
`
`25
`
`0.177
`
`0.167
`
`0.308
`
`0.262
`
`0.752
`
`0.500
`
`1.309
`
`1.110
`
`Prob_Pool
`
`t-Value
`
`OF
`
`StErr-2
`
`StErr-1
`
`StDev-2
`
`StDev-1
`
`0.000
`
`0.000
`
`4.5
`
`5.8
`
`34
`
`25
`
`0.300
`
`0.333
`
`0.243
`
`0.378
`
`1.274
`
`1.000
`
`1.029
`
`1.602
`
`Prob_Pool
`
`t-Value
`
`OF
`
`StErr-2
`
`StErr-1
`
`StDev-2
`
`StDev-1
`
`1.4
`
`1.2
`
`1.2
`
`-2.5
`
`-1.9
`
`-2.5
`
`1.7
`
`3.4
`
`2.1
`
`4.8
`
`4.1
`
`4.6
`
`

`

`United States Department of Agriculture, Agricultural Marketing Service
`Science and Technology, Plant Variety Protection Office
`National Agricultural Library Building, Room 400
`Beltsville, MD 20705-2351
`
`EXHIBITC
`(Corn; Maize)
`(2009)
`
`Name of Applicant(s)
`Pioneer Hi-Bred International,
`
`Inc.
`
`OBJECTIVE DESCRIPTION OF VARIETY
`CORN (Zea mays L.)
`
`Variety Seed Source
`
`Variety Name or Temporary Designation
`PH18KJ
`
`Address (Street & No., or R.F.D. No., City, State, Zip Code and Country
`7300 NW 62nd Avenue, P.O. Box 1004, Johnston,
`Iowa 50131-1004
`
`FOR OFFICIAL USE
`
`I
`
`PVPO Number
`
`#201000366
`
`that describes the varietal characters typical of this inbred variety in the spaces below. Right justify whole numbers by adding
`Place the appropriate number
`leading zeroes if necessary. Completeness
`should be striven for to establish an adequate variety description. Traits designated by a "*" are considered necessary
`for an adequate variety description and must be completed.
`
`COLOR CHOICES (Use in conjunction with Munsell color code to describe all color choices; describe #25 and #26 in Comments section):
`01. Light Green
`06. Pale Yellow
`11. Pink
`16. Pale Purple
`21. Buff
`26. Other (Describe)
`02. Medium Green
`07. Yellow
`12. Light Red
`17. Purple
`22. Tan
`03. Dark Green
`08. Yellow-Orange
`13. Cherry Red
`18. Colorless
`23. Brown
`04. Very Dark Green
`09. Salmon
`14. Red
`19. White
`24. Bronze
`1O. Pink-Orange
`15. Red & White
`05. Green-Yellow
`20. White Capped
`25. Variegated (Describe)
`
`B64,B68
`
`trial data):
`
`lowa5125, P39, 2132
`
`(in background and maturity) of these to make comparisons based on grow-out
`STANDARD INBRED CHOICES [Use the most similar
`Yellow Dent Families:
`Yellow Dent (Unrelated):
`Sweet Corn:
`Family
`Members
`C0109, ND246
`C13,
`B14
`CM105,A632,
`Oh7,T232
`B37
`B37,B76,H84
`W117,W153R
`B73, Nc268
`B73
`N192,A679,
`W182BN
`C103
`M017,Va102,Va35,A682
`Oh43
`A619, MS71, H99, Va26
`WF9
`W64A, A554, A654, Pa91
`
`Popcorn:
`
`SG1533, 4722, HP301, HP7211
`
`White Dent:
`C166, H105, Ky228
`
`Pipecorn:
`M015W, M016W, M024W
`
`1. TYPE:
`
`section)
`(describe intermediate types in "Comments"
`;}. (1=Sweet, 2=Denl, 3=Flint, 4=Flour, 5=Pop, 6=Ornamental,
`
`7=Pipecorn)
`
`Standard Inbred Name
`;}. Type
`
`VA26
`
`2. REGION WHERE DEVELOPED IN THE U.S.A.:
`;}. (1=N.West, 2=N.Central,
`3=N.East, 4=S.East, 5=S.Central, 6=S.West, 7=Other
`
`Standard Seed Source
`_ Region
`
`AMES 19329
`
`show Heat Unit formula in "Comments"
`
`3. MATURITY (In Region Best Adaptability;
`DAYS HEAT UNITS
`63
`1,292.5 From emergence to 50% of plants in silk
`63
`1,292.5 From emergence to 50% of plants in pollen
`~
`41 From 10% to 90% pollen shed
`. _ From 50% silk to optimum edible quality
`____
`_ _ _ _ _ From 50% silk to harvest at 25% moisture
`
`section):
`
`DAYS
`63
`65
`;}.
`
`HEAT UNITS
`1,292.5
`1.327.0
`69
`
`St.Dev.
`4. PLANT:
`5.87
`tip)
`(to tassel
`216.2 cm Plant Height
`7.73
`83.1 cm Ear Height
`(to base of top ear node)
`1.04
`14.8 cm Length of Top Ear Internode
`0.02
`0.0 Average Number of Tillers
`l.Q Average Number of Ears per Stalk
`0.17
`;}. Anthocyanin
`of Brace Roots: 1=Absent, 2=Faint, 3=Moderate, 4=Dark
`
`Sample Size
`18
`.1Ji
`.1Ji
`
`~*~.
`
`Mean
`238.6
`80.2
`17.2
`0.0
`l.Q
`1.
`
`St.Dev.
`11.55
`8.58
`2.38
`0.03
`0.05
`
`Sample Size
`18
`18
`
`18~.
`
`~*
`
`Application Variety Data
`
`Page 1
`
`Standard Inbred Data
`
`EX1009 - Page 8
`
`

`

`Application Variety Data
`
`Page 2
`
`5. LEAF
`8.4 cm Width of Ear Node Leaf
`79.4 cm Length of Ear Node Leaf
`6.2 Number of leaves above top ear
`20.8 Degrees Leaf Angle
`(Measure from 2nd leaf above ear at anthesis to stalk above leaf)
`1 Leaf Color (Munsell Code)
`5GY36
`~ Leaf Sheath Pubescence (Rate on scale from 1=none to 9=like peach fuzz)
`_ Marginal Waves (Rate on scale from 1=none to 9=many)
`_
`Longitudinal Creases (Rate on scale from 1=none to 9=many)
`
`St.Dev.
`0.51
`4.27
`0.71
`3.93
`
`6. TASSEL:
`8.0 Number of Primary Lateral Branches
`26.1 Degrees Branch Angle from Central Spike
`53.1 cm tassel Length
`tip)
`(from top leaf collar to tassel
`2 Pollen Shed (Rate on scale from O=male sterile to 9=heavy shed)
`21 Anther Color (Munsell Code)
`2.5Y84
`14 Glume Color (Munsell Code)
`10RP310
`1 Bar Glumes (Glume Bands): 1=Absent, 2=Present
`
`St.Dev.
`1.85
`4.39
`3.33
`
`Standard InbrIA 2taO1 0 0 0 3 6 6
`
`Sample Size
`1Jl.
`18
`1Jl.
`1Jl.
`
`Mean
`9.2
`81.8
`6.6
`23.9
`
`St.Dev.
`1.20
`5.75
`0.78
`4.39
`
`Sample Size'
`1Jl..
`18
`1Jl.
`1Jl.
`
`1 (Munsell Code)
`~
`
`5GY34
`
`Sample Size
`18
`1Jl.
`1Jl.
`
`Mean
`19.1
`28.9
`57.9
`
`St.Dev.
`3.00
`13.99
`6.28
`
`Sample Size
`1Jl.
`1Jl.
`1Jl.
`
`10Y88
`5GY58
`
`~1
`
`(Munsell Code)
`~ (Munsell Code)
`1
`
`1 Munsell Code
`~ Munsell Code
`~ Munsell Code
`
`2.5GY86
`5GY56
`2.5Y8.52
`
`St.Dev.
`1.17
`2.30
`24.87
`1.28
`
`Sample Size
`1Jl.
`1Jl.
`1Jl.
`18
`
`~2~
`
`Mean
`13.8
`44.3
`120.8
`15.9
`
`~~
`
`8.9
`1
`
`St.Dev.
`1.03
`0.62
`0.62
`4.08
`
`Mean
`11.7
`8.2
`4.5
`36.7
`1 (describe)
`Z Munsell Code
`Z Munsell Code
`~ (describe)
`
`Sample Size
`1Jl.
`18
`1Jl.
`2**
`
`2.5Y814
`2.5Y814
`
`7a. EAR (Unhusked Data):
`10RP38
`(Munsell Code)
`14 Silk Color (3 days after emergence)
`2.5GY66
`~ Fresh Husk Color (25 days after 50% silking)
`(Munsell Code)
`2.5Y92
`~ Dry Husk Color (65 days after 50% silking)
`(Munsell Code)
`1 Position of Ear at Dry Husk Stage: 1=Upright, 2=Horizontal, 3=Pendent
`~ Husk Tightness (Rate on scale from 1=very loose to 9=very tight
`~ Husk Extension (at harvest): 1=Short(ears exposed), 2=Medium «8cm), 3=Long
`(8-10cm beyond ear tip), 4=Very Long (>10cm)
`
`7b. EAR (Husked Ear Data)
`14.2 cm Ear Length
`46.6 mm Ear Diameter at mid-point
`150.7 gm Ear Weight
`16.8 Number of Kernel Rows
`~ Kernel Rows: 1=lndistinct, 2=Distinct
`~ Row Alignment: 1=Straight, 2=Slightly Curved, 3=Spiral
`2.01
`10.8 cm Shank Length
`~ Ear Taper: 1=Slight cyl., 2=Average slightly con., 3=Extreme conical
`
`St. Dev.
`1.31
`1.58
`24.77
`1.40
`
`Sample Size
`18
`1Jl.
`1Jl.
`1Jl.
`
`St.Dev.
`8. KERNEL (Dried):
`0.71
`12.5 mm Kernel Length
`0.77
`8.0 mm Kernel Width
`0.67
`4.7 mm Kernel Thickness
`3.65
`63.5 % Round Kernels (Shape Grade)
`1Aleurone Color Pattern: 1=Homozygous,
`(describe)
`2=Segregating
`Z Aleurone Color (Munsell Code)
`10YR714
`Z Hard Endosperm Color (Munsell Code)
`10YR814
`~ Endosperm Type: 1=Sweet
`(sui), 2=Extra Sweet (sh2), 3=Normal Starch, 4=High
`Amylose Starch, 5=Waxy Starch, 6=High Protein, 7=High Lysine, 8=Super Sweet
`(se), 9=High Oil, 10=Other
`_
`31.0 gm Weight per 100 kernels (unsized sample)
`
`Sample Size
`1Jl.
`18
`18
`
`2**
`
`2**
`
`9. COB:
`27.0 mm Cob Diameter at mid-point
`1Q Cob Color (Munsell Code)
`
`Application Variety Data
`
`StDev.
`1.03
`
`Sample Size
`18
`
`Mean
`25.5
`~ Munsell Code
`
`St.Dev
`0.92
`2.5Y92
`
`Sample Size
`1Jl.
`
`Standard Inbred Data
`
`Note: Use chart on first page to choose color codes for color traits
`
`EX1009 - Page 9
`
`

`

`Application Variety Data
`
`I
`
`Page 3
`
`Standard Inb~
`
`~t«)
`
`1 0 0 0 3 6 6
`
`to 9 (most resistant);
`
`leave blank if not
`
`10. DISEASE RESISTANCE (Rate from 1(most susceptible)
`tested;
`leave Race or Strain Options blank if polygenic):
`A. Leaf Blights, Wilts, and Local
`Infection Diseases
`_ Anthracnose Leaf Blight (Colletotrichum graminicola)
`Common Rust (Puccinia sorghi)
`_ Common Smut (Ustilago maydis)
`Eyespot
`(Kabatiella zeae)
`.!l. Goss's Wilt (Clavibacter michiganense
`spp. nebraskensis)
`Z Gray Leaf Spot (Cercospora zeae-maydis)
`_ Helminthosporium Leaf Spot (Bipolaris zeicola)
`.!l. Northern Leaf Blight (Exserohilum turcicum)
`Southern Leaf Blight (Bipolaris maydis)
`Southern Rust (Puccinia Polysora)
`Stewart's Wilt (Erwinia stewartii)
`_ Other (Specify)
`B. Systemic Diseases
`Corn Lethal Necrosis (MCMV and MDMV)
`Head Smut (Sphacelotheca
`reiliana)
`_ Maize Chlorotic Dwarf Virus (MCDV)
`_ Maize Chlorotic Mottle Virus (MCMV)
`Maize Dwarf Mosaic Virus (MDMV)
`_ Sorghum Downy Mildew of Corn (Peronosclerospora
`_ Other (Specify)
`C. Stalk Rots
`Anthracnose Stalk Rot (Colletotrichum graminicola)
`_ Diplodia Stalk Rot (Stenocarpella maydis)
`_ Fusarium Stalk Rot (Fusarium moniliforme)
`_ Gibberella Stalk Rot (Gibberella zeae)
`_ Other (Specify)
`D. Ear and Kernel Rots
`_ Aspergillus Ear and Kernel Rot (Aspergillus flavus)
`Z Diplodia Ear Rot (Stenocarpella maydis)
`.!l. Fusarium Ear and Kernel Rot (Fusarium moniliforme)
`Gibberella Ear Rot (Gibberella zeae)
`_ Other (Specify)
`
`Strain
`sorghi)
`
`Leaf Blight
`
`_ Anthracnose
`Common Rust
`Common Smut
`Eyespot
`1Goss'sWilt
`1Gray Leaf Spot
`_ Helminthosporium Leaf Spot
`!2 Northern Leaf Blight
`Southern Leaf Blight
`Southern Rust
`Stewart's Wilt
`_ Other (Specify)
`
`_
`
`Race_
`Race_
`Race_
`
`Corn Lethal Necrosis
`Head Smut
`Maize Chlorotic Dwarf Virus
`Maize Chlorotic Mottle Virus
`Strain
`Maize Dwarf Mosaic Virus
`_ Sorghum Downy Mildew of Corn
`_ Other (Specify)
`_
`
`Anthracnose Stalk Rot
`_ Diplodia Stalk Rot
`Fusarium Stalk Rot
`Gibberella Stalk Rot
`_ Other (Specify)
`
`_
`
`_ Aspergillus Ear & Kernel Rot
`!2 Diplodia Ear Rot
`.!l. Fusarium Ear & Kernel Rot
`Gibberella Ear Rot
`Other (Specify)
`
`_
`
`Race
`Race
`Race
`
`_
`_
`_
`
`_
`
`_
`
`_
`
`_
`
`_
`
`Note: Use chart on first page to choose color codes for color traits.
`
`EX1009 - Page 10
`
`

`

`Application Variety Data
`
`Page 4
`
`Standard ~r2
`
`D~ 1 0 0 0, 3. 6 6
`
`to 9 (most resistant); Leave blank
`St. Dev.
`Sample Size
`
`. _mg larval wt.
`
`11. INSECT RESISTANCE (Rate from 1(most susceptible)
`if not tested
`_ Banks Grass Mite (Oligonychus pratensis)
`Corn Earworm (Helicoverpa zeal
`_ Leaf Feeding
`Silk Feeding
`_ Ear Damage
`_ Corn Leaf Aphid (Rhopalosiphum maid is)
`Corn Sap Beetle (Carpophilus dimidiatus)
`_
`European Corn Borer (Ostrinia nubilalis)
`1 st Generation (Typically Whorl Leaf Feeding)
`_ 2 nd Generarion (Typically Leaf Sheath-Collar Feeding)
`Stalk Tunneling:
`. _cm tunneled/plant
`Fall Armyworm (Spodoptera frugiperda)
`_ Leaf-Feeding
`. _mg larval wt.
`Silk-Feeding
`(Sitophilus zeamais)
`_ Maize Weevil
`_ Northern Rootworm (Diabrotica barberi)
`_ Southern Rootworm (Diabrotica undecimpunctata)
`Southwestern Corn Borer (Diatraea grandiosella)
`_ Leaf Feeding
`. _cm tunneled/plant
`Stalk Tunneling:
`_ Two-spotted Spider Mite (Tetranychus urticae)
`_ Western Rootworm (Diabrotica virgifera virgifera)
`_ Other (Specify)
`
`12. AGRONOMIC TRAITS:
`1 Stay Green (at 65 days after anthesis)
`(Rate on scale from 1=worst
`% Dropped Ears (at 65 days after anthesis)
`_ % Pre-anthesis Brittle Snapping
`Q % Pre-anthesis Root Lodging
`% Post-anthesis Root Lodging (at 65 days after anthesis)
`8.465.0 Kg/ha Yield of Inbred Per Se (at 12-13% grain moisture)
`
`_
`
`to 9=excellent)
`
`St. Dev.
`Banks Grass Mite
`Corn Earworm
`_ Leaf Feeding
`
`Sample Size
`
`_ Ear Damage
`_ Corn Leaf Aphid
`_ Corn Sap Beetle
`European Corn Borer
`1 st Generation
`2 nd Generation
`
`Fall Armyworm
`_ Leaf-Feeding
`
`Maize Weevil
`Northern Rootworm
`Southern Rootworm
`Southwestern Corn Borer
`_ Leaf Feeding
`
`_ Two-spotted Spider Mite
`Western Rootworm
`_ Other (Specify)
`
`_
`
`~ Stay Green
`% Dropped ears
`_ % Pre-anthesis Brittle Snapping
`20 % Pre-anthesis Root Lodging
`Post-anthesis Root Lodging
`4.830.0 Yield
`
`13. MOLECULAR MARKERS:
`_ Isozymes
`
`(O=data unavailable; 1=data available but not supplied; 2=data supplied.)
`1 Other (Specify)
`RFLP's
`RAPD's
`
`SNPs
`
`REFERENCES:
`of Corn Inbred Lines. PhD Thesis, Ohio University.
`Butler, D.R. 1954. A System for the Classification
`Emerson, R.A, G.W. Beadle, and AC. Fraser, 1935. A summary of Linkage Studies in Maize. Cornell A.E.S., Mem. 180.
`Farr, D.F., G.F. Bills, G.P. Chamuris, AY. Rossman. 1989. Fungi on Plant Products in the United States. The American Phytopathological
`Inglett, G.E. (Ed) 1970. Corn: Culture, Processing, Products. Avi Publishing Company, Westpoint, CT.
`Jugenheimer, R.W. 1976. Corn:
`Improvement, Seed Production, and Uses. John Wiley & Sons, New York.
`McGee, D.C. 1988. Maize Diseases. APS Press, St. Paul, MN. 150 pp.
`Munsell Color Chart
`for Plant Tissues. Macbeth. P.O. Box 230. Newburgh, N.Y. 12551-0230
`The Mutants of Maize. 1968. Crop Science Society of America. Madison, WI.
`Shurtleff, M.C. 1980. Compendium of Corn Diseases. APS Press, St. Paul, MN. 105 pp.
`Sprague, G.F., and J.W. Dudley (Editors). 1988. Corn and Corn Improvement, Third Edition. Agronomy Monograph 18. ASA, CSSA, SSSA, Madison, WI.
`Stringfield, G.H. Maize Inbred Lines of Ohio AE.S., Bul. 831. 1959.
`U.S. Department of Agriculture 1936, 1937. Yearbook.
`
`Society, St. Paul, MN.
`
`COMMENTS (e. g. state how heat units were calculated, standard inbred seed source, and/or where data was collected. Continue in Exhibit D).
`'Sample number
`reflects the number of plots where the trait(s) was observed and not the number of individual plants scored.
`Please see 'CLARIFICATION OF DATA IN EXHIBITS BAND C' for details of how plots were set up .
`•• For these plot-level
`traits, kernels from approximately
`5 representative ears were sampled. 100 unsized kernels were counted and weighed.
`Up to 500 grams of kernels were sized by a 13/64 inch slot screen.
`
`Insect, disease, brittle snapping, yield and root lodging data are collected mainly from environment where variability for the trait can be obtained
`within the experiment.
`
`EX1009 - Page 11
`
`

`

`lU.,f
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`CLARIFICATION OF DATA IN EXHIBITS BAND C
`
`I ';1 p,(~ i'l,
`
`'\I
`
`''/.'I,~tv'
`
`I h [( ...II'
`
`(
`
`. ,lk:~""fO.-.t,0:0 0 3 6 6
`
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`
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`
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`
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`
`\
`
`;'
`
`~ I
`
`"
`
`:,
`
`Please note the data presented in Exhibit Band C, "Objective Description of Variety," are
`collected primarily at Johnston and/or Dallas Center, Iowa. The data in Table 1 are from two
`sample t-tests using data collected in Johnston and Dallas Center, IA. These traits in Exhibit B
`collectively show distinct differences between the two varieties.
`
`For the given year of data collection, our experimental design was set up in a typical complete block
`design commonly used in agricultural corn research experiments with two replications grown at each
`location. The experimental procedures generally involve two locations/environments with different
`planting dates, planted in 13 or 17.42 ft. rows with 2 row plots (group) for each variety. Approximately
`20-30 plants emerged in each of the 2 rows for a total of around 80 to 120 plants being evaluated at
`each location and 160 to 240 plants across locations. For plant level traits, we sampled up to 18
`representative plants from each location. For plot level traits we evaluated the 2 row plot (group) and
`gave a representative score or average on the 80-120 plants in the group within an experiment.
`
`GROWING DEGREE UNITS (GDUs)
`2009
`
`Month
`May
`June
`July
`August
`September
`TOTAL
`
`Dallas Center
`381
`588
`560
`576
`418
`2523
`
`Johnston
`426
`640
`655
`656
`493
`2870
`
`PRECIPITATION (Inches)
`2009
`
`Dallas Center
`3.67
`4.38
`2.84
`4.96
`1.00
`16.85
`
`Johnston
`3.85
`6.51
`2.40
`4.85
`1.48
`19.09
`
`Growing Degree Units use following formula: GDU = ((Tl +T2)/2)-50
`
`Where Tl = minimum temperature for a given day with 50 degrees Fahrenheit as the minimum
`temperature used and 86 degrees Fahrenheit
`is the maximum temperature used.
`
`Where T2 = maximum temperature for a given day with 86 degrees Fahrenheit as the maximum
`temperature used and 50 degrees Fahrenheit
`is the minimum temperature used.
`
`GDUs are calculated each day and accumulated (summed) over certain number of days.
`
`EX1009 - Page 12
`
`

`

`REPRODUCE LOCALLY I
`I d f
`. nc u e orm num er an e I Ion ate on a repro uctions.
`d dT
`d
`b
`d
`U.S. DEPARTMENT OF AGRICULTURE
`AGRICULTURAL MARKETING SERVICE
`
`EXHIBIT E
`STATEMENT OF THE BASIS OF OWNERSHIP
`1. NAME OF APPLICANT(S)
`
`Pioneer Hi-Bred International,
`
`Inc.
`
`Application is required in order to determine if a plant variety protection
`certificate is to be issued (7 U.S.C. 2421). The information is held
`confidential until the certificate is issued (7 U.S.C. 2426).
`
`2. TEMPORARY DESIGNATION
`OR EXPERIMENTAL NUMBER
`
`3. VARIETY NAME
`
`PH18KJ
`
`FORM APPROVED - OMB No. 0581-0055
`
`I'
`
`4. ADDRESS (Street and No., or RFD. No., City, State, and ZIP, and Country)
`
`5. TELEPHONE (Include area code)
`
`6. FAX (Include area code)
`
`7300 NW 62nd Avenue
`P.O. Box 1004
`Johnston,
`IA 50131-1004
`
`(515) 270-4051
`
`(515) 253-2298
`
`7. PVPO NUMBER
`
`#2 0 1 000366
`YES o NO
`
`~
`
`8. Does the applicant own all rights to the variety? Mark an "X" in the appropriate block.
`
`If no, please explain.
`
`9. Is the applicant a U.S. national or a U.S. based entity? If no, give name of country.
`
`YES o NO
`
`~
`
`10.
`
`Is the applicant
`
`the original owner?
`
`~
`
`YES o NO
`
`If no, please answer one of the following:
`
`a. If the original
`
`rights to variety were owned by individual(s),
`is (are) the original owner(s) a U.S. National(s)?
`0 NO
`DYES
`If no, give name of country
`
`b. If the original
`
`rights to variety were owned by a company(ies),
`is (are) the original owner(s) a U.S. based company?
`YES 0 NO
`~
`If no, give name of country
`
`11. Additional explanation on ownership (Trace ownership from original breeder
`
`to current owner. Use the reverse for extra space if needed):
`
`its wholly owned subsidiary Pioneer Overseas Corporation (POC),
`Iowa, andlor
`Inc. (PHI), Des Moines,
`Pioneer Hi-Bred International,
`Des Moines,
`Iowa,
`is the employer of