200700388
`
`20Alii,NOWHOMULESE:PRESENTS SEAMM,COMET:
`
`
`Pioneer Hi-Bred International, Inc.
`
`. Whereas, THERE HAS BEEN PRESENTED TO THE
`
`Secretary of Agriculture
`
`‘An application requesting a certificate of;:Prote tion for an alleged distinct variety of sexually
`scription of which are contained in the
`
`
`
`
`
`term ofLWENTY years from the dateofthis
`dic repiéhishmentof viable basic seed of the
`
`
`purpo:
`
`érefrom, to the extent providedby, the PLANT VARIETY PROTECTION ACT. (84 STAT. 1542,AS
`
`D, 7 U.S.C. 2321 ET SEQ.)
`ma
`'
`
`CORN, FIELD
`'‘PHEQE!
`
`
`
`In Testimony Whereof, J have hereunto set my
`
`hand and caused the seal of the Plant Variety
`Protection Office to be affixed at the City of |
`Washington, D.C.
`this
`twenty-fifth day of |
`March, in the year two thousand and eleven.
` Attest:
`
`
`
`
`
`Acting Commissioner
`Plant Variety Protection Office
`
`Agricultural Marketing Service
`
`
`
` #
`
`Inari v. Pioneer
`
`Inari Exhibit 1094
`Inari Exhibit 1094
`Inari v. Pioneer
`
`

`

`REPRODUCELOCALLY.Include form number and date onall reproductions
`U.S. DEPARTMENT OF AGRICULTURE
`AGRICULTURAL MARKETING SERVICE
`SCIENCE AND TECHNOLOGY- PLANT VARIETY PROTECTION OFFICE
`
`Thefollowing statements are madein accordancewith the Privacy Act of 1974 (5 U.S.C. 552a) and
`the Paperwork Reduction Act (PRA) of 1995.
`
`Form Approved - OMB No. 0581-0055
`
`Application is required in order to determineif a plant variety protection certificate is to be issued
`APPLICATION FOR PLANT VARIETY PROTECTION CERTIFICATE
`(7 U.S.C. 2421).
`Information is held confidential until certificate is issued (7 U.S.C. 2426).
`(Instructions and information collection burden statement on reverse)
`
`3. VARIETY NAME
`2. TEMPORARY DESIGNATION OR
`1. NAME OF OWNER
`EXPERIMENTAL NAME
`
`
`PHE2E
`Pioneer Hi-Bred International, Inc.
`FOR OFFICIAL USE ONLY
`
`5. TELEPHONE (include area code)
`4. ADDRESS (Street and No., or R.F.D. No., City, State, and ZIP Code, and Country)
`PVPO NUMBER
`
`
`
`7301 NW 62™ Avenue
`Johnston, IA 50131-0085
`
`515/270-4051
`
`6.
`FAX(include area code)
`
`QANTAS Oe
`ah OO /¢ © 3g J
`
`515/253-2125
`
`9. DATE OF INCORPORATION
`8.
`IF INCORPORATED, GIVE
`7.
`IF THE OWNER NAMEDIS NOT A "PERSON", GIVE FORM OF
`STATE OF INCORPORATION
`ORGANIZATION (corporation, partnership, association, etc.)
`lowa
`March 5, 1999
`Corporation
`F
`FILING AND EXAMINATION FEES:
`E
`$ Y3 72,00
`E 7 Int,
`Ss
`
`aL
`DATE
`‘7 ASOT
`CE
`FICATION FFE:
`E
`ane
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`|
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`
`|
`
`
`
`
`
`
`
`
`
`
`
`
`
`(= 10. NAME AND ADDRESS OF OWNER REPRESENTATIVE(S) TO SERVEIN THIS APPLICATION. (First personlisted will receive all papers)
`
`,
`Steven R. Anderson
`“Oo
`a
`Research and Product Development
`,
`wh
`ss
`~
`P.O. Box85 /064
`i
`un
`Johnston, IA 50131-0085 /604
`vt}
`2
`
`VY
`
`12.
`
`43. E-MAIL
`
`FAX(Include area code)
`2 Pt
`41. TELEPHONE(Include area code)
`
`.
`FSS
`steven.anderson@pioneer.com
`515/253-2425-
`515/270-4051
`14. CROP KIND (Common Name)
`16. FAMILY NAME(Botanical)
`18. DOES THE VARIETY CONTAIN ANY TRANSGENES? (OPTIONAL)
`
`no
`&
`Corn
`Gramineae
`O ves
`15. GENUS AND SPECIES NAME OF CROP
`17.
`IS THE VARIETY A FIRST GENERATION HYBRID?
`IF SO, PLEASE GIVE THE ASSIGNED USDA-APHIS REFERENCE NUMBER FOR THE
`
`APPROVEDPETITION TO DEREGULATE THE GENETICALLY MODIFIED PLANT FOR
`
`
`COMMERICALIZATION.
`20. DOES THE OWNER SPECIFY THAT SEED OF THIS VARIETY BE SOLD AS A CLASS
`
`OF CERTIFIED SEED? (See Section 83(a) of the Plant Variety Protection Act)
`
`YES(If "yes", answeritems 21 and 22 below)
`1 NO(If "no", go to item 23)
`21. DOES THE OWNER SPECIFY THAT SEED OF THIS VARIETY BE LIMITED AS TO
`NUMBER OF CLASSES?
`
`
`
`YES
`NO
`
`
`
`
`
`
`
`
`
`
`
`
`
`IF YES, WHICH CLASSES?
`FOUNDATION
`REGISTERED
`CERTIFIED
`22. ile SEED‘OP THISVARIETY BE LIMITED AS TO
`
`.
`
`
`
`YES
`O No
`
`& NO
`YES
`Zea Mays
`19. CHECK APPROPRIATE BOX FOR EACH ATTACHMENT SUBMITTED
`(Follow instructions on reverse)
`a. & Exhibit A. Origin and Breeding History of the Variety
`+0.
`rn
`b. & Exhibit B. Statementof Distinctness
`c. J Exhibit C. Objective Description of Variety
`d. 1 Exhibit D. Additional Description of the Variety (Optional)
`e. &J Exhibit E. Statementof the Basis of the Owner's Ownership
`f. Ej Voucher Sample (3,000 viable untreated seeds or, for tuber propagated varieties,
`verification that tissue culture will be deposited and maintained in an approved public
`repository)
`g. &I Filing and Examination Fee ($4,382), made payable to "Treasurer of the United
`States" (Mail to the Plant Variety Protection Office)
`
`IF-VES, SPECIFY THE NUMBER1,2,, etc. FOR EACH CLASS.
`O FOUNDATION
`LC REGISTERED
`LC CERTIFIED
`
`(If additional explanation is necessary, please use the space indicated on the reverse.)
`23. HAS THE VARIETY (INCLUDING ANY HARVESTED MATERIAL) OR A HYBRID PRODUCED
`24.
`IS THE VARIETY OR ANY COMPONENT OF THE VARIETY PROTECTED BY
`FROM THIS VARIETY BEEN SOLD, DISPOSED OF, TRANSFERRED, OR USEDIN THE U. S. OR
`INTELLECTUAL PROPERTY RIGHT (PLANT BREEDER'S RIGHT OR PATENT)?
`OTHER COUNTRIES?
`
`
`
`
`
`
`& NO
`YES
`NO
`Kl
`YES
`IF YES, YOU MUST PROVIDE THE DATE OFFIRST SALE, DISPOSITION, TRANSFER, OR USE
`IF YES, PLEASE GIVE COUNTRY, DATE OF FILING OR ISSUANCE AND ASSIGNED
`FOR EACH COUNTRY AND THE CIRCUMSTANCES.(Please use spaceindicated on reverse.)
`REFERENCE NUMBER.(Please use spaceindicated on reverse.)
`
`
`
`25. The owners declare that a viable sample of basic seed of the variety has been furnished with application andwill be replenished upon request in accordance with such regulations as may beapplicable, or
`for a tuber propagatedvariety a tissue culture will be deposited in a public repository and maintainedfor the duration of the certificate.
`
`The undersigned owner(s) is(are) the ownerof this sexually reproduced ortuber propagatedplant variety, and believe(s) that the variety is new,distinct, uniform, and stable as required in Section 42, and is
`entitled to protection underthe provisions of Section 42 of the Plant Variety Protection Act.
`Owner(s)is (are) informed that false representation herein can jeopardize protection and result in penalties.
`SIGNATURE OF OWNER
`SIGNATURE OF OWNER
`
`Lk Law 2 Mf
`
`)
`
`oy
`
`LO
`
`;
`
`J
`
`NAME (Pleaseprint ortype)
`
`NAME(Pleaseprint or type)
`
`Steven R. Anderson
`DATE
`CAPACITY OR TITLE
`CAPACITY OR TITLE
`DATE
`
`Research Scientist
`2-/g- 2007
`
`
`(See reversefor instructions and information collection burden statement)
` ST-470 (02-06) designed by the Plant Variety Protection Office using Word 2003.
`
`
`
`

`

`GENERAL INSTRUCTIONS:Tobeeffectively filed with the PlantVariety Protection Office (PVPO), ALL ofthe rotowile 20.0 Zc.0, DindoVeo:
`
`(1) Completed application form signed by the owner; (2) completed exhibits A, B, C, E, F; (3) for a tuber reproducedvariety, verification that a viable (in the
`sense thatit will reproduce an entire plant) tissue culture will be deposited and maintained in an approved public repository; and (4) paymentby credit card or
`check drawn on a U.S. bank for $4,382 ($518 filing fee and $3,864 examination fee), payable to "Treasurer of the United States" (See Section 97.6 of the
`Regulations and Rules of Practice.) NEW: With the application for a seed reproduced variety or by direct deposit soonafterfiling, the applicant must
`provideat least 3,000 viable untreated seedsofthe variety perse, and for a hybrid variety at least 3,000 untreated seedsof eachline necessary to reproduce
`the variety. Partial applications will be held in the PVPOfor not morethan 90 days; then returned to the applicant as un-filed. Mail application and other
`requirements to Plant Variety Protection Office, AMS, USDA, Room 401, NAL Building, 10301 Baltimore Avenue, Beltsville, MD 20705-2351. Retain one copy
`for your files. All items on the face of the application are self explanatory unless noted below. Corrections on the application form and exhibits mustbeinitialed
`and dated. DO NOTuse masking materials to make corrections.
`If a certificate is allowed, you will be requested to send a paymentby credit card or check
`payable to "Treasurer of the United States" in the amount of $768 for issuance ofthe certificate. Certificates will be issued to owner,notlicensee or agent.
`
`NOTES: It is the responsibility of the applicant/owner to keep the PVPOinformed of any changesof addressor changeof ownership or assignmentor owner's representative during the
`life of the application/certificate. The feesforfiling a change of address; owner's representative, ownershipor assignment; or any modification of owner's nameis specified in Section
`97.175 ofthe regulations. (See Section 101 of the Act, and Sections 97.130.97.131, 97.175(h) ofthe Regulations and Rulesof Practice.)
`
`Plant Variety Protection Office
`FAX: (301) 504-5291
`Telephone: (301) 504-5518
`General E-mail: PYVPOmail@usda.gov
`Homepage: http://www.ams.usda.gov/science/pvpo/PVPindex.htm
`
`SPECIFIC INSTRUCTIONS:
`To avoid conflict with other variety namesin use, the applicant must check the appropriate recognized authority and provide evidencethat the permanent
`nameofthe application variety (evenif it is a parental, inbredline) has been cleared by the appropriate recognized authority before the Certificate of Protection
`is issued. For example, for agricultural and vegetable crops, contact: U.S. Departmentof Agriculture, Agricultural Marketing Service, Livestock and Seed
`Programs, Seed Regulatory and Testing Branch, 801 Summit Crossing Place, Suite C, Gastonia, North Carolina 28054-2193 Telephone: (704) 810-8870.
`http://www.ams.usda.gov/Isg/seed.htm.
`
`ITEM
`19a.Give:
`
`(1) the genealogy, including public and commercialvarieties,lines, or clones used, and the breeding method;
`(2) the details of subsequent stages of selection and multiplication;
`(3) evidence of uniformity and stability; and
`(4) the type and frequencyof variants during reproduction and multiplication and state how these variants maybeidentified
`
`19b.Give a summaryofthe variety's distinctness. Clearly state how this application variety may be distinguished from all other varieties in the same crop.
`newvariety is most similar to one variety or a group ofrelated varieties:
`
`If the
`
`(1) identify these varieties andstate all differences objectively;
`(2) attach replicated statistical data for characters expressed numerically and demonstratethat these are clear differences; and
`(3) submit, if helpful, seed and plant specimens or photographs (prints) of seed and plant comparisons whichclearly indicate distinctness.
`
`19c.Exhibit C forms are available from the PVPO Office for most crops; specify crop kind. Fill in Exhibit C (Objective Description of Variety) form as completely
`as possible to describe your variety.
`19d.Optional additional characteristics and/or photographs. Describe any additional characteristics that cannot be accurately conveyedin Exhibit C. Use
`comparativevarieties as is necessary to reveal more accurately the characteristics that are difficult to describe, such asplanthabit, plant color, disease
`resistance, etc.
`
`19e.Section 52(5) of the Act requires applicants to furnish a statementof the basis of the applicant's ownership. An Exhibit E form is available from the PVPO.
`
`20.
`
`If "Yes" is specified (seedof this variety be sold by variety nameonly, as a class ofcertified seed), the applicant MAY NOTreversethis affirmative
`decision after the variety has been sold andso labeled, the decision published, or the certificate issued. However,if "No" has been specified, the applicant
`may change the choice. (See Regulations and Rules of Practice, Section 97.103).
`
`|
`|
`|
`
`|
`
`|
`
`|
`
`23. See Sections 41, 42, and 43 of the Act and Section 97.5 of the regulationsfor eligibility requirements.
`
`24. See Section 55 of the Actfor instructions on claiming the benefit of an earlierfiling date.
`
`22. CONTINUED FROM FRONT (Please provide a statement asto the limitation and sequence of generations that may becertified.)
`
`23. CONTINUED FROM FRONT(Pleaseprovide the date offirst sale, disposition, transfer, or use for each country and the circumstances,if the variety
`(including any harvested material) or a hybrid produced from this variety has been sold, disposedof, transferred, or usedin the U.S.or other countries.)
`
`Meeeeeeeeeeeeeeeeee
`24. CONTINUED FROM FRONT(Pleasegive the country, dateoffiling or issuance, and assigned reference number,if the variety or any componentofthe
`variety is protected by intellectual property right (Plant Breeder's Right or Patent).)
`
`ee
`According to the Paperwork Reduction Act of 1995, an agency may not conductor sponsor, and a personis not required to respondto a collection ofinformation unlessit displays a valid OMBcontrol number. The
`valid OMB control numberforthis information collection is 0581-0055. The time required to complete this information collection is estimated to average 1.4 hours per response, including the time for reviewing
`instructions, searching existing data sources, gathering and maintaining the data needed, and completing and reviewing thecollection ofinformation.
`
`The U.S. Departmentof Agriculture (USDA) prohibits discrimination in all its programsandactivities on the basis of race, color, nationalorigin, age, disability, and where applicable, sex, marital status or familial
`status, parental status, religion, sexual orientation, genetic information, political beliefs, reprisal, or becauseall or part ofan individual's incomeis derived from any public assistance program (Notall prohibited bases
`apply to all programs.) Personswith disabilities who require alternative means for communication of program information (Braille, large print, audiotape, etc.) should contact USDA's TARGETCenterat 202-720-
`2600 (voice and TDD).
`
`Tofile a complaint of discrimination, write USDA,Director, Office of Civil Rights, 1400 Independence Avenue, S.W., Washington, DC 20250-9410 orcall (800) 795-3272 (voice) or (202) 720-6382125
`(TDD). USDAis an equal opportunity provider and employer.
`
`
` ST-470 (02-06) designed by the Plant Variety Protection Office using Word 2003.
`
`
`

`

`#2007003 88
`
`Exhibit A. Origin and Breeding History for PHE2E
`
`Pedigree: PH6JG/PH5W4)XA42313121X
`
`Pioneer Line PHE2E, Zea mays L., a yellow endospermcorn inbred, was developed by PioneerHi-
`Bred International, Inc. from the single cross hybrid PH6JG X PHSW4(PVP Certificate No.
`200100244) using the pedigree method of plant breeding. Varieties PH6JG and PH5W4are
`proprietary inbred lines of Pioneer Hi-Bred International, Inc. Variety PH6JG wasderived by pedigree
`selection from the single cross hybrid PHOJTX PHHB9 (PVP Certificate No. 9300108). Selfing was
`practiced from the above hybrid for 10 generations using pedigree selection. During line development,
`crosses were madeto inbred testers for the purpose of estimating the line's combiningability. Yield
`trials were grown at Union City, Tennessee as well as other Pioneer research locations. After initial
`testing, additional hybrid combinations have been evaluated and subsequent generationsofthe line
`have been grown and hand-pollinated with observations again made for uniformity.
`
`Variety PHE2E has shown uniformity and stability forall traits as described in Exhibit C - "Objective
`Description of Variety". It has been self-pollinated and ear-rowed 8 generations with careful attention
`paid to selectioncriteria and uniformity ofplant type to assure genetic homozygosity and phenotypic
`stability. The line has been increased both by hand andinisolated fields with continued observations
`for uniformity and stability, and for 5 generations during the final stages of inbred development and
`seed multiplication. Very high standards for genetic purity have beenestablished morphologically
`using field observations and using sound lab molecular marker methodology.
`
`No variant traits have been observed or are expected in PHE2E.
`
`Thecriteria used in the selection of PHE2E wereyield, both per se and in hybrid combinations. Late
`seasonplant health, grain quality, and stalk lodging resistance, were important criteria considered
`during selection. Other selection criteria include: ability to germinate in adverse conditions, disease
`and insect resistance, pollen yield andtasselsize.
`
`*
`
`PHOST was derived from PHBA3 X PHPB3. PHBA3wasderived from PHW52 (PVP
`Certificate No. 8800215) X PHK29 (PVP Certificate No. 8700214). PHPB3 was derived
`from PHV35 X PHM24. PHV35 wasderived from G39 (PVP Certificate No. 8300115) X
`B73. PHM24 wasderived from Variety 422 and B64. Variety 422 was primarily derived
`from a cross of B37 with Variety 938. Variety 938 is a line tracing back to C103 and
`Mp3204.
`
`3
`
`

`

`Exhibit A: Developmental History for PHE2E
`
`#200700388
`
`
`
`Inbreeding Level of
`Pedigree Grown
`F
`
`0 0
`
`F
`
`
`
`
`
`
`
`
`
`Pedigree Grown
`Season/Year
`
`PH6JG
`1998
`PHSW4
`1998
`
`
`
`1
`
`2
`
`F F
`
`F3
`
`F4
`
`FS
`
`F6
`
`F7
`
`F8
`
`F9
`
`FIO
`
`Fil
`(SEED)
`
`PH6JG/PHS W4
`1998
`PH6JG/PH5 W4)X
`1999
`PH6JG/PH5W4)XA4
`2000
`
`2001
`
`2001
`
`PH6JG/PH5W4)XA423 1
`2002
`
`PH6JG/PHSW4)XA423 13
`2002
`
`PHOJG/PH5W4)XA423131
`2003
`
`PH6JG/PHS5 W4)XA4231312
`
`PH6JG/PH5W4)XA42313121
`2004
`
`PH6JG/PH5W4)XA42313121X
`
`*PHEZ2Ewasselfed and ear-rowed from F3 through F9 generation.
`#Uniformity and stability were established from F7 through F11 generation and beyond
`whenseed supplies were increased.
`
`

`

`#2007003 88
`
`Exhibit B: Novelty Statement
`
`Variety PHE2E mostly resembles Pioneer Hi-Bred International, Inc. proprietary inbred line PHSW4
`(PVP Certificate No. 200100244). Table 1 shows two sample t-tests on data collected primarily in
`Johnston and Dallas Center, Iowa in 2005 and 2006. Thetraits collectively show measurable
`differences betweenthe two varieties.
`
`Exhibit B: Novelty Statement
`
`Variety PHE2E hasa greater ear diameter (45.0 mm vs 40.4 mm) than variety PHSW4 (Table1).
`
`Variety PHE2E hasa greaterearinternode length (16.2 cm vs 12.2 cm) than variety PHSW4(Table 1).
`
`Variety PHE2Ehasa greater kernel length (12.3 mm vs 10.6 mm)than variety PHSW4 (Table1).
`
`Variety PHE2E hasa greater kernel width (8.9 mm vs 7.7 mm) than variety PHSW4(Table 1).
`
`Variety PHE2E hasa greater plant height (244.0 cm vs 209.6 cm) than variety PHSW4 (Table 1).
`
`Variety PHE2E hasa shortertassel central spike length (27.5 cm vs 32.2 cm) than variety PHS W4
`(Table 1).
`
`

`

`#200700388
`
`
`
`
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`

`

`United States Department of Agriculture, Agricultural Marketing Service
`Science and Technology, Plant Variety Protection Office
`National Agricultural Library Building, Room 400
`Beltsville, MD 20705-2351
`
`EXHIBIT C
`(Corn; Maize)
`(8-22-2001)
`
`OBJECTIVE DESCRIPTION OF VARIETY
`CORN (Zea maysL.)
`
`Nameof Applicant(s)
`|
`Variety Seed Source
`| Variety Name or Temporary Designation
`Pioneer Hi-Bred International, Inc
`|
`|
`PHE2E
`
`Address (Street & No., or R.F.D. No., City, State, Zip Code and Country
`|
`FOR OFFICIAL USE
`|
`PYVPQ Number
`
`
`
`| #7301 NW 62nd Avenue, P.O. Box 85, Johnston, lowa 50131-0085 i 7 0 0 3 8 8
`
`
`
`
`
`}
`|
`t
`
`I
`
`|
`}
`
`}
`|
`}
`
`Place the appropriate numberthat describes the varietal characterstypical of this inbred variety in the spaces below.Rightjustify whole numbers by
`adding leading zeroesif necessary. Completeness should bestriven for to establish an adequate variety description. Traits designated by a "*" are
`considered necessary for an adequate variety description and must be completed.
`
`
`COLOR CHOICES(Usein conjunction with Munsell color code to describe all color choices; describe #25 and #26 in Comments section):
`01. Light Green
`06. Pale Yellow
`11. Pink
`16. Pale Purple
`21. Buff
`26. Other (Describe)
`02. Medium Green
`07. Yellow
`12. Light Red
`17. Purple
`22. Tan
`03. Dark Green
`08. Yellow-Orange
`13. Cherry Red
`18. Colorless
`23. Brown
`04. Very Dark Green
`09. Salmon
`14. Red
`19. White
`24. Bronze
`05. Green-Yellow
`10. Pink-Orange
`15. Red & White
`20. White Capped
`25. Variegated (Describe)
`
`STANDARD INBRED CHOICES[Usethe mostsimilar (in background and maturity) of these to make comparisons based on grow-outtrial data]:
`Yellow Dent Families:
`Yellow Dent (Unrelated):
`SweetCorn:
`Family
`Members
`C0109, ND246
`C13, lowa5125, P39, 2132
`B14
`CM105, A632, B64, B68
`Oh7, T232
`B37
`B37, B76, H84
`W117, W153R
`B73
`N192, A679, B73, Nc268
`W182BN
`C103
`Mo17, Va102, Va35, A682
`Oh43
`A619, MS71, H99, Va26
`White Dent:
`Pipecorn:
`WF9
`W64A, A554, A654, Pa91
`Cl66, H105, Ky228
`Mo15W, Mo16W, Mo24W
`
`1. TYPE: (describe intermediate types in "Comments" section)
`|
`Standard Inbred Name
`B73
`3 (1=Sweet, 2=Dent, 3=Flint, 4=Flour, 5=Pop, 6=Ornamental, 7=Pipecorn)
`|
`3 Type
`|
`
`2. REGION WHERE DEVELOPED IN THE U.S.A.:
`|
`Standard Seed Source
`PI 550473
`4 (1=N.West, 2=N.Central, 3=N.East, 4=S.East, 5=S.Central, 6=S.West, 7=Other
`|
`_ Region
`
`3. MATURITY(In Region Best Adaptability; show Heat Unit formula in "Comments" section):
`|
`HEAT UNITS
`DAYS
`DAYS HEAT UNITS
`|
`
`
`1,436.4
`62
`65
`1,508.6 From emergence to 50% ofplantsin silk
`I
`
`1,397.4
`61
`63
`1,462.8 From emergence to 50% ofplants in pollen
`|
`59
`2
`2
`56 From 10% to 90% pollen shed
`___ ____._ From 50% silk to optimum edible quality
`oe
`eres Bee
`___ ____._ From 50% silk to harvest at 25% moisture
`_
`epeecenyess
`Boxe
`
`
`Popcorn:
`
`$G1533, 4722, HP301, HP7211
`
`|||
`
`St.Dev.
`4. PLANT:
`11.99
`244.0 cm Plant Height (to tassel tip)
`8.86
`87.6 cm Ear Height (to base of top ear node)
`1.94
`16.2 cm Length of Top Ear Internode
`0.02
`0.0 Average Number ofTillers
`0.20
`41.1 Average Number of Ears per Stalk
`4 Anthocyanin of Brace Roots: 1=Absent, 2=Faint, 3=Moderate, 4=Dark
`
`Sample Size |
`25 |
`25 |
`25:
`|
`5 |
`5 |
`|
`
`Mean
`241.4
`103.0
`16.6
`0.0
`141
`
`St.Dev.
`9.35
`10.28
`Mal
`0.00
`0.07
`
`Sample Size
`25
`25
`25
`5
`5
`
`3
`
`Application Variety Data
`
`Page 1
`
`|
`
`Standard Inbred Data
`
`

`

`#200700588
`Application Variety Data
`Page 2
`|
`Standard Inbred Data
`
`5. LEAF
`St.Dev.
`Sample Size |
`Mean
`St.Dev.
`Sample Size
`9.6 cm Width of Ear Node Leaf
`0.65
`25 |
`9.0
`0.68
`25
`86.8 cm Length of Ear Node Leaf
`5.43
`25 |
`86.9
`3.91
`25
`7.1 Numberof leaves above top ear
`0.76
`25 |
`6.2
`0.76
`25
`26.1 Degrees Leaf Angle
`3.96
`25 |
`27.4
`4.60
`29
`(Measure from 2nd leaf above ear at anthesis to stalk aboveleaf)
`|
`4 (Munsell Code) 7.5GY34
`4 Leaf Color (Munsell Code)
`5GY34
`|
`4
`2 Leaf Sheath Pubescence (Rate on scale from 1=noneto 9=like peach fuzz)
`|
`__
`__ Marginal Waves(Rate on scale from 1=none to 9=many)
`|
`__ Longitudinal Creases (Rate on scale from 1=none to 9=many)
`|
`__
`
`
`St.Dev.
`1.80
`11.00
`3.30
`
`Mean
`5
`21.6
`56.2
`
`St.Dev.
`4.53
`8.43
`3:25
`
`Sample Size
`25
`25
`25
`
`6. TASSEL:
`5.4 Numberof Primary Lateral Branches
`42.8 Degrees Branch Angle from Central Spike
`58.8 cm tassel Length
`(from top leaf collar to tasseltip)
`5 Pollen Shed (Rate on scale from O=malesterile to 9=heavy shed)
`12 Anther Color (Munsell Code)
`2.5R58
`14 Glume Color (Munsell Code)
`7.5RP38
`4 Bar Glumes (Glume Bands): 1=Absent, 2=Present
`
`Sample Size |
`25 |
`25 |
`25 |
`|
`|
`|
`|
`|
`|
`a. EAR (Unhusked Data):
`1 Munsell Code
`|
`5R58
`12 Silk Color (3 days after emergence) (Munsell Code)
`
`2 Munsell Code
`|
`5GY68
`2 Fresh Husk Color (25 days after 50%silking) (Munsell Code)
`
`21 Munsell Code
`|
`2.5Y84
`21 Dry Husk Color(65 days after 50% silking) (Munsell Code)
`Z
`|
`1 Position of Ear at Dry Husk Stage: 1=Upright, 2=Horizontal, 3=Pendent
`v
`|
`7 Husk Tightness (Rate on scale from 1=very loose to 9=verytight
`2 Husk Extension(at harvest): 1=Short(ears exposed), 2=Medium (<8cm), 3=Long (8-
`|
`3
`10cm beyondear tip), 4=Very Long (>10cm)
`|
`
`7b. EAR (Husked Ear Data)
`St. Dev.
`Sample Size |
`Mean
`St.Dev.
`Sample Size
`14.9 cm Ear Length
`1.04
`25 |
`14.1
`0.64
`25
`45.0 mm EarDiameter at mid-point
`1.34
`25 |
`46.3
`1.97
`25
`138.4 gm Ear Weight
`19.2
`25 |
`131.4
`19.59
`25
`14.1 Numberof Kernel Rows
`0.70
`25 |
`18.0
`1.41
`25
`2 Kernel Rows: 1=Indistinct, 2=Distinct
`|
`2
`2 Row Alignment: 1=Straight, 2=Slightly Curved, 3=Spiral
`|
`2
`25
`2.19
`1.54
`8.7 cm Shank Length
`25 |
`10.3
`2 Ear Taper: 1=Slight cyl., 2=Average slightly con., 3=Extreme conical
`|
`2
`
`8. KERNEL (Dried):
`St.Dev.
`Sample Size |
`Mean
`St.Dev
`Sample Size
`12.3. mm Kernel Length
`0.68
`25 |
`11.2
`0.76
`25
`8.9 mm Kernel Width
`0.70
`25:
`|
`C3)
`0.56
`25
`0.56
`4.3 mm Kernel Thickness
`25 |
`4.4
`0.71
`25
`
`15.49
`41.4 % Round Kernels (Shape Grade)
`5 |
`30.8
`6.51
`5
`1 Aleurone Color Pattern: 1=Homozygous, 2=Segregating (describe)
`|
`1 (describe)
`7 Aleurone Color (Munsell Code)
`10YR714
`|
`7 Munsell Code
`7 Hard Endosperm Color (Munsell Code)
`10YR610
`|
`7 Munsell Code
`3 Endosperm Type: 1=Sweet(su1), 2=Extra Sweet(sh2), 3=Normal Starch, 4=High
`|
`3 (describe)
`Amylose Starch, 5=Waxy Starch, 6=High Protein, 7=High Lysine, 8=Super Sweet
`|
`(se), 9=High Oil, 10=Other.
`|
`33.8 gm Weight per 100 kernels (unsized sample)
`1.64
`5 |
`27.0
`2.35
`
`9. COB:
`St.Dev.
`Sample Size |
`Mean
`St.Dev
`Sample Size
`23.4 mm Cob Diameterat mid-point
`0.71
`25 |
`27.6
`0.95
`25
`2.5YR38
`14 Cob Color (Munsell Code)
`|
`11 Munsell Code
`10R66
`
`Standard Inbred Data
`Application Variety Data
`
`Note: Use chart on first page to choosecolor codesforcolortraits
`
`5
`7 (Munsell Code) 5Y8.54
`
`2 (Munsell Code) 5GY56
`
`2.5GY94
`
`5GY78
`2.5Y8.54
`
`1 7
`
`amr
`
`2.5Y812
`10YR712
`
`5
`
`

`

`Page 3
`Application Variety Data
`
`
`#20N7TNDNABB
`
`Standard Inbred Data
`
`Race
`Race
`Race.
`
`_ Anthracnose LeafBlight
`Common Rust
`_ Common Smut
`Eyespot
`Goss's Wilt
`3 Gray Leaf Spot
`_ Helminthosporium Leaf Spot Race__
`3 Northern Leaf Blight
`Race___
`Southern Leaf Blight
`Race___
`Southern Rust
`5 Stewart's Wilt
`_ Other (Specify)
`
`10. DISEASE RESISTANCE(Rate from 1(most susceptible) to 9 (mostresistant); leave blank
`if not tested; leave Race or Strain Options blank if polygenic):
`A. Leaf Blights, Wilts, and Local Infection Diseases
`_ Anthracnose Leaf Blight (Colletotrichum graminicola)
`6 CommonRust (Puccinia sorghi)
`9 Common Smut (Ustilago maydis)
`Eyespot(Kabatiella zeae)
`Goss's Wilt (Clavibacter michiganense spp. nebraskensis)
`5 Gray Leaf Spot (Cercospora zeae-maydis)
`_ Helminthosporium Leaf Spot (Bipolaris zeicola)
`5 Northern Leaf Blight (Exserohilum turcicum)
`7 Southern LeafBlight (Bipolaris maydis)
`Southern Rust (Puccinia Polysora)
`6 Stewart's Wilt (Erwinia stewartii)
`
`_ Other (Specify)
`B. Systemic Diseases
`Corn Lethal Necrosis (MCMV and MDMV)
`9 Head Smut (Sphacelothecareiliana)
`_ Maize Chlorotic Dwarf Virus (MCDV)
`_ Maize Chlorotic Mottle Virus (MCMV)
`Maize Dwarf Mosaic Virus (MDMV)
`Strain
`_ Sorghum Downy Mildew of Corn (Peronosclerospora sorghi)
`
`_ Other (Specify)
`C. Stalk Rots
`6 AnthracnoseStalk Rot (Colletotrichum graminicola)
`_ Diplodia Stalk Rot (Stenocarpella maydis)
`_ Fusarium Stalk Rot (Fusarium moniliforme)
`_ Gibberella Stalk Rot (Gibberella zeae)
`
`_ Other (Specify)
`D. Ear and Kernel Rots
`Aspergillus Ear & Kernel Rot
`_ Aspergillus Ear and Kernel Rot (Aspergillus flavus)
`Diplodia Ear Rot
`5 Diplodia Ear Rot (Stenocarpella maydis)
`Fusarium Ear & Kernel Rot
`5 Fusarium Ear and Kernel Rot (Fusarium moniliforme)
`Gibberella Ear Rot
`Gibberella Ear Rot (Gibberella zeae)
`Other (Specify)
`_ Other (Specify)
`
`
`Corn Lethal Necrosis
`9 Head Smut
`_ Maize Chlorotic Dwarf Virus
`_ Maize Chlorotic Mottle Virus
`Strain
`Maize Dwarf Mosaic Virus
`_ Sorghum Downy Mildew of Corn
`_ Other (Specify)
`
`3 Anthracnose Stalk Rot
`Diplodia Stalk Rot
`Fusarium Stalk Rot
`Gibberella Stalk Rot
`_ Other (Specify)
`
`Ion[e9|
`
`Note: Use chartonfirst page to choose color codesfor colortraits.
`
`k
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`i
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`

`

`HOPNN7TANSBS
`Standard Inbred Data
`|
`Page 4
`Application Variety Data
`
`11. INSECT RESISTANCE (Rate from 1(most susceptible) to 9 (most resistant); Leave blank
`if not tested
`St. Dev.
`Sample Size
`_ Banks Grass Mite (Oligonychuspratensis)
`Corn Earworm (Helicoverpa zea)
`_ Leaf Feeding
`Silk Feeding ___._mg larval wt.
`_ Ear Damage
`_ Corn Leaf Aphid (Rhopalosiphum maidis)
`____ Corn Sap Beetle (Carpophilus dimidiatus)
`European Corn Borer (Ostrinia nubilalis)
`1 st Generation (Typically Whorl Leaf Feeding)
`_ 2nd Generarion (Typically Leaf Sheath-Collar Feeding)
`Stalk Tunneling:
`__ _ ._cm tunneled/plant
`Fall Armyworm (Spodoptera frugiperda)
`_ Leaf-Feeding
`Silk-Feeding __ _ ._mg larval wt.
`_ Maize Weevil (Sitophilus zeamais)
`
`St. Dev.
`_ Banks Grass Mite
`Corn Earworm
`_ Leaf Feeding
`
`Sample Size
`
`_ Ear Damage
`_ Corn Leaf Aphid
`_ Corn Sap Beetle
`European Corn Borer
`1 st Generation
`_ 2nd Generation
`
`Fall Armyworm
`_ Leaf-Feeding
`
`_ Maize Weevil
`
`_ Northern Rootworm
`|
`_ Northern Rootworm (Diabrotica barberi)
`_ Southern Rootworm
`|
`_ Southern Rootworm (Diabrotica undecimpunctata)
`Southwestern Corn Borer (Diatraea grandiosella) ||Southwestern Corn Borer |
`
`
`_ Leaf Feeding
`|
`_ Leaf Feeding
`i
`_._cem tunneled/plant
`Stalk Tunneling:
`|
`i
`_ Two-spotted Spider Mite
`_ Two-spotted Spider Mite (Tetranychusurticae)
`|
`_ Western Rootworm
`_ Western Rootworm (Diabroticavirgifera virgifera)
`|
`
`_ Other (Specify)
`_ Other (Specify)
`|
`
`12. AGRONOMIC TRAITS:
`|
`3 Stay Green
`7 Stay Green (at 65 days after anthesis) (Rate on scale from 1=worst to 9=excellent)
`|
`% Dropped ears
`% Dropped Ears (at 65 days after anthesis)
`|
`_ % Pre-anthesis Brittle Snapping
`_ % Pre-anthesis Brittle Snapping
`|
`15 % Pre-anthesis Root Lodging
`OQ % Pre-anthesis Root Lodging
`7 Post-anthesis Root Lodging
`7 % Post-anthesis Root Lodging (at 65 daysafter anthesis)
`
`
` 6,010.0 Kg/ha Yield of Inbred Per Se (at 12-13% grain moisture) 5,507.0 Yield
`
`
`||I
`
`wom
`
`13. MOLECULAR MARKERS: (0=data unavailable; 1=data available but not supplied; 2=data supplied.)
`_ Other (Specify) 1 lsozymes _ RFLP's _ RAPD's
`REFERENCES:
`
`
`
`
`
`
`
`
`
`Butler, D.R. 1954. A System for the Classification of Corn Inbred Lines. PhD Thesis, Ohio University.
`Emerson, R.A., G.W. Beadle, and A.C. Fraser, 1935. A summary of Linkage Studies in Maize. Cornell A.E.S., Mem. 180.
`Farr, D.F., G.F. Bills, G.P. Chamuris, A.Y. Rossman. 1989. Fungi on Plant Products in the United States. The American Phytopathological
`Society, St. Paul, MN.
`
`Inglett, G.E. (Ed) 1970. Corn: Culture, Processing, Products. Avi Publishing Company, Westpoint, CT.
`Jugenheimer, R.W. 1976. Corn: Improvement, Seed Production, and Uses. John Wiley & Sons, New York.
`McGee, D.C. 1988. Maize Diseases. APS Press, St. Paul, MN. 150 pp.
`Munsell Color Chart for Plant Tissues. Macbeth. P.O. Box 230. Newburgh, N.Y. 12551-0230
`The Mutants of Maize. 1968. Crop Science Society of America. Madison, WI.
`Shurtleff, M.C. 1980. Compendium of Corn Diseases. APS Press, St. Paul, MN. 105 pp.
`Sprague, G.F., and J.W. Dudley (Editors). 1988. Corn and Corn Improvement, Third Edition. Agronomy Monograph 18. ASA, CSSA, SSSA,
`Madison, WI.
`
`Stringfield, G.H. Maize Inbred Lines of Ohio A.E.S., Bul. 831. 1959.
`U.S. Department of Agriculture 1936, 1937. Yearbook.
`
`
`COMMENTSce. g. state