U.S. Serial No.: 15/632,497
`Atty. Docket No.: GWLG-017US-CIP
`Response to Non-Final Office Action
`
`AMENDMENT TO THE CLAIMS
`
`This listing of claims will replace all prior versions, and listings, of claims in the
`
`application. The following amendments do not constitute an admission regarding the
`
`patentability of the amended subject matter and should not be so construed.
`
`Please amend the claims as follows:
`
`1.
`
`(Currently Amended) A method of diagnosing and treating endometriosis in a mammal
`
`comprising the steps of:
`
`detecting circulating levels of glycodelin and one or more of BDNF, ZAG and
`
`CA125 in a plasma sample from the mammal by contacting the sample with a glycodelin
`
`antibody and an antibody to one or more of BDNF, ZAG and CA125, detecting binding
`
`between glycodelin and one or more of BDNF, ZAG and CA125 and determining
`
`circulating levels thereof based on the amount of antibody binding,
`
`comparing the level of glycodelin and one or more of BDNF, ZAG and CA125 to
`
`a pre-determined level associated with endometriosis;
`
`diagnosing the mammal with endometriosis when the levels of glycodelin and one
`
`or more of BDNF, ZAG and CA125 is elevated to the predetermined level associated with
`
`endometriosis; and
`
`treating the mammal with at least one of a non-steroidal anti-inflammatory drug
`
`[[pai—n—medieatienfl, hormone therapy, a gonadotropin-releasing hormone (Gn-RH) agonist
`
`or antagonist to block the production of ovarian-stimulating hormones, progestin therapy,
`
`or steroid treatment to suppress the growth of the endometrium.
`
`
`Atty. Docket No.: GWLG-017US-CIP
`Response to Non-Final Office Action
`
`AMENDMENT TO THE CLAIMS
`
`This listing of claims will replace all prior versions, and listings, of claims in the
`
`application. The following amendments do not constitute an admission regarding the
`
`patentability of the amended subject matter and should not be so construed.
`
`Please amend the claims as follows:
`
`1.
`
`(Currently Amended) A method of diagnosing and treating endometriosis in a mammal
`
`comprising the steps of:
`
`detecting circulating levels of glycodelin and one or more of BDNF, ZAG and
`
`CA125 in a plasma sample from the mammal by contacting the sample with a glycodelin
`
`antibody and an antibody to one or more of BDNF, ZAG and CA125, detecting binding
`
`between glycodelin and one or more of BDNF, ZAG and CA125 and determining
`
`circulating levels thereof based on the amount of antibody binding,
`
`comparing the level of glycodelin and one or more of BDNF, ZAG and CA125 to
`
`a pre-determined level associated with endometriosis;
`
`diagnosing the mammal with endometriosis when the levels of glycodelin and one
`
`or more of BDNF, ZAG and CA125 is elevated to the predetermined level associated with
`
`endometriosis; and
`
`treating the mammal with at least one of a non-steroidal anti-inflammatory drug
`
`[[pai—n—medieatienfl, hormone therapy, a gonadotropin-releasing hormone (Gn-RH) agonist
`
`or antagonist to block the production of ovarian-stimulating hormones, progestin therapy,
`
`or steroid treatment to suppress the growth of the endometrium.
`
`
`
`US. Serial No.: 15/632,497
`Atty. Docket No.: GWLG-017US-CIP
`Response to Non-Final Office Action
`
`(Previously Presented) The method of claim 1, wherein the level of glycodelin associated
`
`with endometriosis is 39 ng/ml or greater.
`
`(Previously Presented) The method of claim 1, wherein the circulating levels of ZAG and
`
`CA125 are also determined in the sample and compared to a pre-determined level
`
`associated with endometriosis.
`
`(Previously Presented) The method of claim 1, wherein the circulating level of BDNF is
`
`determined in the sample.
`
`(Previously Presented) The method of claim 1, wherein circulating levels of ZAG and
`
`BDNF are determined.
`
`(Previously Presented) The method of claim 1, wherein enzyme-linked immunosorbent
`
`assay is used to detect circulating levels of glycodelin and one or more of BDNF, ZAG and
`
`CA125.
`
`7-12.
`
`(Cancel)
`
`13.
`
`(Previously Presented) A method comprising:
`
`contacting a sample from a mammal with a glycodelin antibody and an antibody to
`
`one or more of BDNF, ZAG and CA-125;
`
`detecting binding between the glycodelin antibody and glycodelin and between the
`
`antibody to one or more of BDNF, ZAG and CA-125 and one or more of BDNF, ZAG and
`
`CA-125, respectively;
`
`determining levels of glycodelin and one or more of BDNF, ZAG CA-125 in the
`
`sample based on the antibody binding; and
`
`
`
`US. Serial No.: 15/632,497
`Atty. Docket No.: GWLG-017US-CIP
`Response to Non-Final Office Action
`
`comparing the levels of glycodelin and one or more of BDNF, ZAG CA-125 with
`
`predetermined levels of glycodelin and one or more of BDNF, ZAG and CA-125, wherein
`
`the predetermined levels are associated with endometriosis.
`
`(Previously Presented) The method of claim 13, wherein levels of ZAG and CA125 are
`
`determined in the sample.
`
`(Previously Presented) The method of claim 13, wherein the level of circulating BDNF is
`
`determined in the sample.
`
`(Previously Presented) The method of claim 15, wherein the level of ZAG is determined
`
`in the sample.
`
`(Previously presented) A method of determining levels of circulating BDNF and CA-125
`
`in a mammal comprising:
`
`detecting the presence of BDNF and CA-125 in a plasma sample from the mammal
`
`by contacting the sample with an anti-BDNF antibody and an anti-CA-125 antibody;
`
`detecting binding between the anti-BDNF antibody and BDNF and between the
`
`anti-CA-125 antibody and CA-125 in the sample; and
`
`determining the circulating levels of BDNF and CA-125 in the mammal based on
`
`the amount of antibody binding.
`
`(New) The method of claim 1, wherein treating the mammal comprises administering a
`
`non-steroidal anti-inflammatory drug to the mammal.
`
`(New) The method of claim 1, wherein treating the mammal comprises administering a
`
`gonadotropin-releasing hormone (Gn-RH) agonist or antagonist to block the production of
`
`ovarian-stimulating hormones to the mammal.
`
`14.
`
`15.
`
`16.
`
`17.
`
`18.
`
`19.
`
`
`
`US. Serial No.: 15/632,497
`Atty. Docket No.: GWLG-017US-CIP
`Response to Non-Final Office Action
`
`20.
`
`21.
`
`22.
`
`23.
`
`24.
`
`25.
`
`(New) The method of claim 1, wherein treating the mammal comprises administering
`
`progestin therapy to the mammal.
`
`(New) The method of claim 1, wherein treating the mammal comprises administering a
`
`steroid to the mammal.
`
`(New) The method of claim 2, wherein treating the mammal comprises administering a
`
`non-steroidal anti-inflammatory drug to the mammal.
`
`(New) The method of claim 2, wherein treating the mammal comprises administering a
`
`gonadotropin-releasing hormone (Gn-RH) agonist or antagonist to block the production of
`
`ovarian-stimulating hormones to the mammal.
`
`(New) The method of claim 2, wherein treating the mammal comprises administering
`
`progestin therapy to the mammal.
`
`(New) The method of claim 2, wherein treating the mammal comprises administering a
`
`steroid to the mammal.
`
`
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