Patent Application
`Atty. Docket No. GWLG-017US-CIP
`
`Figure 1
`
`Circulating BDNF in Women With and Without Endometriosis
`
`2500
`
`P = 0.001
`
`2000
`
`(ngmL)
`
`BDNF
`
`1500
`
`1000
`
`500
`
`Control
`
`Case
`
`N=25
`
`N 41
`
`1/21
`
`

`

`Patent Application
`Atty. Docket No. GWLG-017US-CIP
`
`Figure 2
`
`Circulating BDNF in Women With and Without Endometriosis
`
`by Stage of Disease
`
`*
`
`P = 0.001
`
`2500
`
`2000
`
`1500
`
`1000
`
`500
`
`
`
`BDNF(pglmL)
`
`Control
`
`Stage 1&2
`
`Stage 3&4
`
`N : 25
`
`N = 6
`
`N = 35
`
`2/21
`
`

`

`Patent Application
`Atty. Docket No. GWLG-017US-CIP
`
`Figure 3
`
`Plasma BDNF in Cases
`
`1600
`
`
`
`
`
`PlasmaBDNF(pg/mL)
`
`1400
`
`1200
`
`1000
`
`200
`
`800
`
`600
`
`400
`
`Menses
`
`Follicular
`
`Luteal
`
`3/21
`
`

`

`Patent Application
`Atty. Docket No. GWLG-017US-CIP
`
`Figure 4
`
`Effect of Treatment on Circulating BDNF concentrations
`
`in Women With and Without Endometriosis
`
`*
`
`*
`
`P = <o.oo1
`
`2500
`
`2000
`
`1500
`
`1000
`
`500
`
`
`
`BDNF(pglmL)
`
`
`
`Control
`
`Untreated
`
`Treated
`
`N = 25
`
`N = 41
`
`N = 17
`
`4/21
`
`

`

`Patent Application
`Atty. Docket No. GWLG-017US-CIP
`
`Figure 5
`
`Relationship between Circulating BDNF Concentrations and Pain
`
`in Women with Untreated Endometriosis
`
`N=40
`2
`
`R = 0.0115
`
`.
`
`.
`
`P = 0.510
`
`o 5
`
`/21
`
`2500
`
`2000
`
`1500
`
`1000
`
`500
`
`
`
`BDNF(pg/mL)
`
`

`

`Patent Application
`Atty. Docket No. GWLG-017US-CIP
`
`
` PrmBQN1‘”
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`6/21
`
`

`

`Patent Application
`Atty. Docket No. GWLG-017US-CIP
`
`Fig u re 7
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`7/21
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`

`

`Patent Application
`Atty. Docket No. GWLG-017US-CIP
`
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`

`

`Patent Application
`Atty. Docket No. GWLG-017US-CIP
`
`Figure 9
`
`
`
`
`1 AVDMSGGTVT VLEKVPVSKG QLKQYFYETK CNPMGYTKEG CRGIDKRHWN SQCRTTQSYV
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`9/21
`
`

`

`Patent Application
`Atty. Docket No. GWLG-017US-CIP
`
`Figure 10
`
`1 tgcagtggac atgtcgggcg ggacggtcac agtccttgaa aaggtccctg tatcaaaagg
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`61
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`181
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`
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`61
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`181
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`
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`181
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`ctcgcaa:gc cgaactaccc aatcgtatgt
`
`
`10/21
`
`

`

`
`
`Patent Application
`Atty. Docket No. GWLG-017US-CIP
`
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`

`

`Patent Application
`Atty. Docket No. GWLG-017US-CIP
`
`Figure 12
`
`§\\\
`
`ZAG <— 91.58
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`
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`
`12/21
`
`

`

`Patent Application
`Atty. Docket No. GWLG-017US-CIP
`
`Figure 13
`
`Node 1
`Class = 1
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`
`13/21
`
`

`

`Patent Application
`Atty. Docket No. GWLG-017US-CIP
`
`Figure 14
`
`A)
`
`B)
`
`C)
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`
`14/21
`
`

`

`Patent Application
`Atty. Docket No. GWLG-017US-CIP
`
`Figure 14 cont’d
`
`
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`
`
`
`tggctccaga
`ggcg:ggccc
`gagc:cccaa
`c:ca:ggcga
`gaggacaacc
`g:cc:tggag
`gaggccacgc
`accaccccca
`
`
`
`
`
`gaga:ca:gc
`ctggact:ga
`cctCCZggc
`cag:gggac
`
`:ca:c:g:g'
`
`:ccctggggg
`aaaaaaaaa
`
`
`
`
`
` (I(I(I
`
`gctcagagcc
`:gg:ctgtgg
`agt:ggcagg
`cac:gaaggc
`:ggagatcg:
`agaagac:ga
`
`:gc:cga:ac
`:ccagagca:
`agggat2ca:
`aacaga:gga
`cacctccgcc
`tcctCCchc
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`acccacagcc
`:gtcccggcc
`gacctggcac
`ccc:ctgagg
`:ctgcacaga
`gaa:ccaaag
`:gac:acgac
`
`ga:g:gccag
`cagggct:tc
`agagccg:gc
`:ccaggaaga
`CZttcaaaga
`
`:chthch
`ggcagaggtt
`18)
`
`
`
`
`
`gcagccatgc
`a:ggaca:cc
`:cca:ggcca
`g:ccaca:ca
`:gggagaaca
`aag::caaga
`aa:::cc:gt
`
`
` :acc:ggcca
`
`
`
`
`aggcccc:gc
`cg:::c:agg
`
`ccagac:ccc
`a:aaccacag
`acacc:gcac
`
`aZtaaZaaac
`
`
`
`15/21
`
`tgtgcctcct
`cccagaccaa
`:ggcgaccaa
`cctcactgt:
`acagc:g:g:
`
`:caac:a:ac
`:tc:c:gcc:
`
`gag:cctgg:
`ccaggcacc:
`:gagc:cc:g
`accct2ccac
`
`
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`ctcagaagac
`cacggcca:g
`ccttggagca
`
`
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`

`

`A)
`
`B)
`
`C)
`
`D)
`
`Patent Application
`Atty. Docket No. GWLG-017US-CIP
`
`Figure 15
`
`1 mvrmvpvlls lllllgpavp qenngrysl tyiytglskh vedvpafqal gslndqufr
`61 ynskdrksqp mglquvegm edwkquqlq karedifmet 1kdiveyynd sngshvlqgr
`121 fgceiennrs sgafwkyyyd gkdyiefnke ipavafdpa aqitquwea epvyvqraka
`181 yleeecpat; rkylkyskni 1drqdppsvv vtshqapgek kklkclaydf ypgkidvhwt
`241 ragevqepe; rgdvlhngng tyqswvvvav ppthapysc thhsslaqp 1vvaeas
`
`(SEQ ID NO: 19)
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`1 ccattggcct gtagattcac ctcccctggg cagggcccca ggacccagga taatatctgt
`61 gcc:cc:gcc cagaaccc:c caagcagaca caa:ggtaag aaZggtgcc: g:cctgctgt
`
`
`121 c:c:gc:gc: gct:ctgggt cctgctg:cc cccaggagaa ccaagatgg: cg::actc:c
`181 :gacctata: Ctacactggg Ctgtccaagc atgttgaaga Cgtccccgcg :Ztcaggccc
`
`
`
`241 :Zggctcac: caatgacctc cagttcttta gatacaacag :aaagacagg aagtctcagc
`30" ccatgggact Ctggagacag giggaaggaa tggaggattg gaagcaggac agccaacttc
`361 agaaggccag ggaggacatc :Ztatggaga ccctgaaaga catcgtggag :attacaacg
`421 acagtaacgg gtctcacgta :chagggaa ggtttggttg :gagatcgag aataacagaa
`481 gcagcggagc a::ctggaaa :a:tacta:g aZggaaagga ctacaZZgaa :tcaacaaag
`541 aaatcccagc Ctgggtcccc :tcgacccag cagcccaga: aaccaagcag aagtgggagg
`60" cagaaccagt Ctacgtgcag nggccaagg Citacctgga ggaggagtgc cctgcgactc
`661 :gcggaaata CCZgaaatac agcaaaaa:a tcctggaccg gcaaga2cct ccc:c:gtgg
`721 :ggtcaccag ccaccaggcc ccaggagaaa agaagaaac: gaagtgcctg gcctacgac:
`
`781 :ctacccagg gaaaattgat gtgcactgga thgggccgg Cgaggtgcag gagcctgag:
`
`841 :acggggaga tg:tc::cac aatggaaa:g gcacttacca gtcc:ggg:g nggtggcag
`90" :gcccccgca ggacacagcc ccctactcct gccacgtgca gcacagcagc Ctggcccagc
`961 ccctchggt gccctgggag gccagCZagg aagcaagggt tggaggcaat g:ggga:ctc
`
`1021 agacccagta gctgcccttc Ctgcctgatg :gggagctga accacagaaa icacagtcaa
`
`1081 :ggatccaca aggcctgagg agcagtgtgg ggggacagac aggaggtgga :Ztggagacc
`
`
`1141 gaagaCZggg atgcc:g:ct tgagtagact :ggacccaaa aaatca2ctc acc:tgagcc
`
`1201 cacccccacc ccattgtc:a a:ctg:agaa gctaataaat aatcatccct cc::gcctag
`1261 cataaaaaaa aaaaaaaa
`(SEQ ID NO: 20)
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`1 mvpvllslpl llgpaquet gsyyltflyt glsrpskgfp rfqataflnd qaffhynsns
`61 gkaepvgpws qvegmedwek esqlqraree iflvtlkdim dyykdttgsh tfqgmfgcei
`121 tnnrssgavw ryaydgedfi efnkeipawi pldpaaantk 1kweaekvyv qrakayleee
`181 cpemlkryln ysrshldrid pptvtitsrv ipggnrifkc laygfypqri slhwnkankk
`241 1afepergvf pngngtylsw aevevqudi dpffclidhr gfsqslquw drtrkvkden
`
`301 nvvaqpq (SEQ ID NO: 21)
`
`1 tttcctgtgt agactgttct cccgggcact accgtagcaa tggtgcctgt cctgctgtcc
`61 ctgcctctcc ttctgggtcc tgcagtcttt caggagactg ggtcttatta tctgaccttt
`121 ctctacaccg ggttgtccag acccagcaaa ggttttccga ggtttcaagc cactgcattt
`181 ctcaatgacc aggccttctt ccactacaac agcaacagcg ggaaggcaga gcctgtggga
`241 ccttggagcc aggtggaagg aatggaggac tgggagaagg aaagccagct
`tcagagggcc
`301 agggaggaga tcttccttgt gaccctgaaa gacatcatgg actattacaa ggacactaca
`361 gggtctcaca cctt:caggg aatgtttggt tgcgagatca caaataacag aagtagtgga
`421 gctgtctgga ggta:gcc:a cgacggagag gatttcatcg aattcaacaa agaaatccca
`481 gcttggatcc ccttagaccc agcagctgca aacaccaagc taaagtggga agcagaaaag
`541 gtctacgtgc agcgagccaa ggcataccta gaggaggagt gtcctgaaat gctgaagagg
`601 tacctgaact acag:cga:c tcacctggac cgaatagatc ctcccactgt gacaatcacc
`661 agccgtgtga tcccaggagg aaacagaata ttcaaatgcc tggcctatgg cttctaccca
`721 caaagaatta gtctgcac:g gaacaaggcc aacaagaagc tagcatttga accagaaaga
`781 ggtgtttttc ccaa:ggaaa tggcacttac ctctcctggg cagaggtgga agtctcccca
`841 caggacatag accccttc:t ctgcctcata gatcacaggg ggttttccca atctctctcg
`901 gtgcagtggg ataggacaag aaaagtaaag gatgaaaaca atgttgtagc tcagcctcag
`961 taagttaccc tctc:gcc:g acatgagaga ggtgaacttc agaagtcaat gtcatcaaca
`1021 aggtcttcca tgggccac:g tacaacagcc agcaagaatc caaggaagag gcatgggcac
`1081 agaagacttg aatgccacag actgagttca ctctcaatgt cagatcaatc gccttgcctt
`1141 gtaaacctcc tcct:gat:a atctgtcaac cctcacaatt gccctcatgc ctagaacagc
`
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`16/21
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`

`

`1201 acagaaagga aggcatttta aactcagaga tgctagagaa gtgtgagttg attttatcat
`1261 gtattctgcc cccacatact tgattcaatt gtgaacatct tcatattcac tcaa
`
`(SEQ ID NO: 22)
`
`Patent Application
`Atty. Docket No. GWLG-017US-CIP
`
`17/21
`
`

`

`Patent Application
`Atty. Docket No. GWLG-017US-CIP
`
`Figure 16
`
`e)
`
`[Fe€(CN{6]3/4g([F€( CN)--6]4/3
`
`
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` \\\\\\+ BDNF
`
`
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`-BDNF
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`Current/le-SA
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`Potential / V
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`18/21
`
`

`

`Patent Application
`Atty. Docket No. GWLG-017US-CIP
`
`Figure 17
`
`
`
`
`
`:2-
`
`[£168th
`
`19/21
`
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`
`
`
`
`PercentageDifference(%)
`
`Patent Application
`Atty. Docket No. GWLG-017US-CIP
`
`Figure 18
`
`
`
`[BDNF](ng/mL)
`
`20/21
`
`

`

`Patent Application
`Atty. Docket No. GWLG-Ol7US-CIP
`
`Figure 19
`
`
`
`
`
` — Probe----------- Paaiiive Control
`
`0.6
`
`0.25 ng/mL
`
`6.0X10E-5
`
`
`
`Current/A
`
`4.0X10E-5
`
`2.0X10E-5
`
`Potential / V
`
`8.0X10E-5 ’
`
`1.0 ng/mL
`
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`
`6.0X10E-5 *
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`4.0X10E-5 7
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`2.0x10E-5 7
`
`0.2
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`0.4
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`Potential / V
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`
`
`,,
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`2.0 ng/mL
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`0,0
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`0.0
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`8.0X10E-5
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`6.0X10E-5 '
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`Current/A
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`4.0X10E-5
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`2.0X10E-5
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`0.0
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`Potential / V
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`21/21
`
`