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`AMENDMENTSTO THE SPECIFICATION
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`METHODS AND COMPOSITIONS FOR EXPRESSION CASSETTES COMPRISING
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`A MAIZE GENE-DERIVED INTRON WFFH-SUBSTIFUTEDELEMENTSFOR
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`ENHANCED EXPRESSION
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`The ViennaRNApackageversion 1.8.2 is used to calculate the free energy for each
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`input sequence. (Hofackeret al. Monatshefte f. Chemie 125: 167-188 (1994);
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`aweew-tbi.univie.ac.at/RNA/). The parameter settings for calculation were “-p0-d2,” which
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`turns on calculation of the partition function and ensures that the partition function and the
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`minimum free energy treat dangling end energies in the same manner. Calculation of the
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`energies was executed on the in-house Linux cluster. In this manner, the free energy value and
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`free energy per base pair were determined for each intron. The free energy score refers to the
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`delta-G reported by the tool. The following reference is provided for background on howfree
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`energies are calculated: Expanded Sequence Dependence of Thermodynamic Parameters
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`Improves Prediction of RNA Secondary Structure
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`J. Mol. Biol. 288, 911-940 (1999).
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